Micronuclei kinetics of exfoliated urothelial cells

Lehucher-Michel, Marie-Pascale; Amara-Mokrane, Y.Ait; Devictor, B; Catilina, P.; Botta, A.

doi:10.1016/0027-5107(95)00232-4

Cited by 15 publications

(3 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The tubes were centrifuged at 3000 g for 15 min at 30 C. The pellets were dissolved in 1Â PBS (137 mM/L NaCl, 2.7 mM/L KCl, 10 mM/L Na 2 HPO 4 , 1.8 mM KH 2 PO 4 ) and centrifuged at 1500 g for 10 min at 30 C. The supernatant was discarded without disturbing the pellet that contained the urothelial cells. The pellets were fixed in Carnoy I fixative solution (methanol and glacial acetic acid, 3:1) and stored at 4 C for further use (Lehucher-Michel et al, 1996).…”

Section: Extraction Of Urothelial Exfoliated Cellsmentioning

confidence: 99%

Genotoxicity evaluation of metformin and glimepiride by micronucleus assay in exfoliated urothelial cells of type 2 diabetes mellitus patients

Harishankar

Logeshwaran

Sujeevan

et al. 2015

Food and Chemical Toxicology

View full text Add to dashboard Cite

Section: Extraction Of Urothelial Exfoliated Cellsmentioning

confidence: 99%

Genotoxicity evaluation of metformin and glimepiride by micronucleus assay in exfoliated urothelial cells of type 2 diabetes mellitus patients

Harishankar

Logeshwaran

Sujeevan

et al. 2015

Food and Chemical Toxicology

View full text Add to dashboard Cite

“…If bacterial culture not included to E. coli, the samples were evaluated control group (Figure 1). Epithelial cells were isolated from urine samples by centrifugation and slides prepared according the method previously described by Lehucher-Michel et al [7]. In order to minimize degenerative cell changes, urine samples were collected in sterile plastic bottles and stored at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Escherichia coli'nin Üriner Sistem Epitelyal Hücrelerinde Mikronükleus Testi ile Genotoksik Etkisinin İncelenmesi

Sancer

2018

SDÜ Tıp Fakültesi Dergisi

View full text Add to dashboard Cite

ÖzAmaç: E. coli, kadınlarda idrar yolu enfeksiyonlarına neden olan bir bakteri türüdür. Bu çalışmada kadınların idrar yolu epitel hücrelerinde, enfeksiyona bağlı DNA hasar etkilerinin araştırılması amaçlandı. Metot: 40 E. coli enfeksiyonu olan ve 40 enfeksiyon olmayan kadınların idrar yolu epitel hücreleri, toplandı ve mikronükleus analizine tabi tutuldu. Her iki grupta mikronükleus ve binükleus parametreleri genom stabilitesinin değerlendirilmesi için kullanıldı. Tüm idrar örneklerinin pH, lökosit, lökosit esteraz, nitrit değerleri H-800 ve FUS-200 tam otomatik idrar cihazı ile analiz edildi. Bulgular: E. coli enfeksiyonu olan ve enfeksiyon olmayan grupların sonuçları istatistiksel olarak Mann-Whitney U ve Independent Sample T testi ile değerlendirildi. Mikronükleus, binukleus, idrar, lökosit, lökosit esteraz ve nitrit parametreleri için sonuçlar istatistiksel olarak anlamlı bulundu (p<0,05). pH sonuçları ise istatistiksel olarak anlamlı bulunmadı (p>0,05).Sonuç: İdrar yolu epitel hücrelerinde bulunan E. coli' nin, DNA hasarını indüklediği görülmüştür. Antibiyotik tedavisinin hastalarda erken kullanımı ve uygun dozda kullanımı ile DNA hasar seviyesini azaltmaya yardımcı olabileceği söylenebilir. AbstractObject: E. coli is a type of bacterium that causes urinary tract infections in women. The aim of the work was to investigate the possible use of epithelial cells from the urinary tract in identifying infections-related DNA damage effects in women. Methods: Epithelial cells from urinary tract were collected from 40 E .coli infection and 40 control (non-infection) women and subjected to micronucleus assay. Number of cells with micronucleated, binucleated cells were counted in two groups as parameters for the evaluation of genome stability. All of urine samples pH, leukocyte, leukocyte esterase, nitrite results measured with H-800 urine analyzer and FUS-200 urine sediment analyzer. Result: E. coli infection and non-infection groups test results were statistically evaluated with Mann-Whitney U and Independent Sample T test. A p-value of <0.05 was found statistically significant for micronucleus, binucleus, and urine , leukocyte, leukocyte esterase and nitrite parameters. pH results was not found statistically significant (p>0,05). Conclusion: The detection of E. coli in urinary tract epithelial cells showed that induce DNA damage. It can ÖZGÜN ARAŞTIRMA / ORIGINAL RESEARCH Med J SDU / SDÜ Tıp Fak Derg 2018:25(3):298-302 299 be said that patients with early antibiotics and doses, early treatment may help to reduce the level of DNA damage.

show abstract

“…As urine of males contains considerably less cells than urine of females, we modified the isolation procedure of Lehucher-Michel et al (1996) to minimize cell loss. Urine samples were first centrifuged at 400 × g for 10 min in 250 ml centrifuge plastic bottle (Fisher Scientific Company, Ottawa, On, Canada).…”

Section: Isolation Of Exfoliated Cells In Urinementioning

confidence: 99%

Optimizing urothelial cell preparation for the human urinary micronucleus assay

Fortin

Anghel

Brochu

et al. 2010

Toxicology in Vitro

View full text Add to dashboard Cite

Biological monitoring of early genotoxic effects in urothelial cells using the urinary micronucleus (MNu) assay is promising for early detection of cancer, such as bladder carcinoma. But many problems are encountered, the major being the poorly differential staining of cells, particularly in women having an important amount of squamous cells. We have optimized the protocol and obtained a differential staining of the cell types present in urine on 10 subjects. Following Carnoy I fixation and Papanicolaou staining, urothelial cells were blue while most squamous cells were pink. This differential staining allowed for optimization of the MNu assay on a single urine void, for both females and males. Even if our MNu means were comparable to the literature, the great variation in reported MNu results could reside in the ability of scorers to distinguish correctly between urothelial and squamous cells. When monitoring exposed populations, this erroneous distinction could largely influence the results, even more in women's urine samples. Given a situation where exposure would not increase micronuclei frequency in vaginal squamous cells, their erroneous analysis in the MNu assay could mask an early genotoxic effect. Therefore, as transitional cell carcinoma of the bladder originates from transformed urothelial cells, restricting micronuclei analysis to urothelial cells could yield a more precise estimate of cancer risk in exposed populations. Moreover, it is hoped that the improvements proposed in this paper will allow for an easier implementation of the MNu assay in various set-ups and enhance its specificity, since MNu are considered a suitable biomarker.

show abstract

Micronuclei kinetics of exfoliated urothelial cells

Cited by 15 publications

References 18 publications

Genotoxicity evaluation of metformin and glimepiride by micronucleus assay in exfoliated urothelial cells of type 2 diabetes mellitus patients

Genotoxicity evaluation of metformin and glimepiride by micronucleus assay in exfoliated urothelial cells of type 2 diabetes mellitus patients

Escherichia coli'nin Üriner Sistem Epitelyal Hücrelerinde Mikronükleus Testi ile Genotoksik Etkisinin İncelenmesi

Optimizing urothelial cell preparation for the human urinary micronucleus assay

Contact Info

Product

Resources

About