Micronucleus and mutation assessment by flow cytometry: Integration into rodent repeat-dose studies

Bemis, Jeffrey C.; Phonethepswath, Souk; Franklin, Dean; Raja, Sarojini; Torous, Dorothea K.; Bryce, Steven M.; Avlasevich, Svetlana L.; Dertinger, Stephen D.

doi:10.1016/j.toxlet.2009.06.735

Cited by 1 publication

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“…In a study comparing the level of damaged cells in adult, sub-adult, and juvenile rodents to identify possible relationships between age and DNA impairment, increases in the level of damaged cells associated with age were found for wild rodents in coal regions [ 21 ]. In a study assessing micronucleus formation in rats after chronic exposure to DEP, no sex-based differences in MN frequency were observed [ 22 ]. The current understanding of the common variables (age, gender) effect of PM 2.5 induced DNA damage is unclear and limited.…”

Section: Introductionmentioning

confidence: 99%

Age and Gender Effects on Genotoxicity in Diesel Exhaust Particles Exposed C57BL/6 Mice

et al. 2021

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There is growing evidence that the accumulation of DNA damage induced by fine particulate matter (PM2.5) exposure is an underlying mechanism of pulmonary disease onset and progression. However, there is a lack of experimental evidence on whether common factors (age, gender) affect PM2.5 induced genomic damage. Here, we assessed the DNA damage potency of PM2.5 using conventional genotoxicity testing in old male and female mice aged 8 and 40 weeks. Mice were intratracheally instilled with diesel exhaust PM2.5 (DEP, NIST SRM 1650b), twice a week for 4 weeks. Exposure to DEP was not associated with an increase in the frequency of micronucleated polychromatic erythrocytes and did not induce a systemic genotoxic effect in the bone marrow. Meanwhile, the results from the comet assay showed a significant increase in DNA damage in DEP exposed mouse lung specimens. The positive relationship between DEP exposure and DNA damage is stronger in the older than in the younger group. Statistical analysis showed that there was a modifying effect of age on the association between PM2.5 exposure and DNA damage. Our results suggest that the age factor should be considered to better understand the cellular adverse effects of PM2.5.

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Section: Introductionmentioning

confidence: 99%