Micronucleus evaluation in mitogen‐stimulated lymphocytes of narrow‐band (311 nm TL01) UVB‐treated patients

Ferahbaş, Ayten; Dönmez-Altuntaş, Hamiyet; Hamurcu, Zühal; Aktaş, Ekrem; Utaş, Serap

doi:10.1111/j.1600-0781.2004.00086.x

Cited by 8 publications

(9 citation statements)

References 23 publications

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“…Until now, the genotoxic or cytogenetic damage results of vitiligo and similar diseases have generally been on the effects of different agents used in the treatment of skin diseases. [12][13][14] The present study will be the first report concerning the MN frequency in vitiligo patients. Therefore, we have aimed to investigate spontaneous MN frequency with cytokinesis block MN assay in phytohaemagglutinin (PHA)-stimulated blood cells of vitiligo patients.…”

Section: Introductionmentioning

confidence: 70%

“…Our investigation shows that MN evaluation has not been undertaken in lymphocytes from vitiligo patients, associated with disease aetiology. Until now, the genotoxic or cytogenetic damage results of vitiligo and similar diseases have generally been on the effects of different agents used in the treatment of skin diseases 12–14 . The present study will be the first report concerning the MN frequency in vitiligo patients.…”

Section: Introductionmentioning

confidence: 77%

“…Those phototherapies are associated with acute and chronic side‐effects on human skin, despite beneficial effects, of UV radiation 12 . UVB and PUVA exposure for the therapy of many skin diseases such as psoriasis and vitiligo causes DNA damage at the molecular level 13,14 . Ultimately, the accumulation of genetic changes may play an important role in the development of skin cancers.…”

Section: Discussionmentioning

confidence: 99%

“…12 UVB and PUVA exposure for the therapy of many skin diseases such as psoriasis and vitiligo causes DNA damage at the molecular level. 13,14 Ultimately, the accumulation of genetic changes may play an important role in the development of skin cancers. But there is no significantly increased risk for sun-induced damage and skin cancer in patients with vitiligo.…”

Section: Discussionmentioning

confidence: 99%

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Increased micronucleus frequency in phytohaemagglutinin‐stimulated blood cells of patients with vitiligo

Dönmez-Altuntaş

Sut

Ferahbaş³

et al. 2007

Acad Dermatol Venereol

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show abstract

Section: Introductionmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Increased micronucleus frequency in phytohaemagglutinin‐stimulated blood cells of patients with vitiligo

Dönmez-Altuntaş

Sut

Ferahbaş³

et al. 2007

Acad Dermatol Venereol

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“…The micronuclei assay can also be used to assess the effect of UV-B radiation treatment of patients with skin diseases (Ferahbas et al 2004). It could be shown that in lymphocytes isolated from their blood exposure to narrowband UV-B radiation in several treatment sessions leads to significant levels of damage.…”

Section: Micronucleimentioning

confidence: 99%

Effects of Ultraviolet Radiation on DNA

Kiefer¹

Chromosomal Alterations

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UV radiation is selectively absorbed by DNA, mainly in the UV-B and the UV-C regions. Excitation of DNA leads to typical photoproducts, the most important being pyrimidine dimers and the so-called 6-4-photoproduct. The yield of strand breaks is very low directly after exposure, but they may be formed indirectly upon further incubation. Although DNA absorbs only weakly in the UV-A region, it may be damaged indirectly via endogenous photosensitisers. Photoproducts are subject to repair processes which are genetically determined. Photoreactivation splits selectively pyrimidine dimers but it is limited to lower eukaryotes and does not occur in mammalian cells. Nucleotide excision repair acts on various types of damage. UVinduced damage manifests itself also at the level of chromosomes. Chromatid-type aberrations are more frequent with UV irradiation than chromosomal aberrations. The action spectrum for their formation demonstrates that an indirect mechanism plays an important role in the UV-A region. Also micronuclei are found after broadband UV exposure which are presumably due to indirectly formed double-strand breaks. UV radiation is a strong inducer of sister-chromatid exchanges, in contrast to ionising radiation, where the yields are low. This suggests that strand breaks play only a minor role.

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Genotoxic effect of simultaneous therapeutic exposure to polycyclic aromatic hydrocarbons and UV radiation

Málková

Borská

Šmejkalová

et al. 2020

J of Applied Toxicology

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Polycyclic aromatic hydrocarbons (PAHs) and ultraviolet radiation (UV) represent genotoxic factors that commonly occur in the living and working environment. The dermal form of exposure represents a significant part of the total load of dangerous chemical and physical environmental factors to which an organism is subjected. However, simultaneous dermal exposures to PAHs (pharmaceutical crude coal tar [CCT]) and UV (UVA and UVB) also have therapeutic uses. A typical example is Goeckerman therapy (GT) for psoriasis. The question of the therapeutic efficacy of GT and the related level of genotoxic danger is still under discussion. The aim of the present study was to compare four GT variants (G1–G4) in terms of efficacy and acceptable genotoxic hazard. Efficacy was expressed by the psoriasis area of severity index (PASI) score, genotoxic hazard by chromosomal aberration in peripheral lymphocytes. The lowest risk of genotoxic hazard and the lowest efficiency was observed in G1 variant (3% of the CCT and UVA + UVB). The efficacy of G2 (4% CCT and UVA + UVB), G3 (4% CCT and UVB), and G4 variants (5% CCT and UVA + UVB) was comparable. The highest risk of genotoxic hazard was found in the G3 variant. In the terms of sufficient efficacy and acceptable genotoxic hazard, a combination of 4% or 5% of CCT and UVA and UVB seems to be acceptable (variants G2 and G4).

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Micronucleus evaluation in mitogen‐stimulated lymphocytes of narrow‐band (311 nm TL01) UVB‐treated patients

Abstract: The results of this study show that narrow-band UVB treatment causes a detectable chromosome damaging effect.

Cited by 8 publications

References 23 publications

Increased micronucleus frequency in phytohaemagglutinin‐stimulated blood cells of patients with vitiligo

Increased micronucleus frequency in phytohaemagglutinin‐stimulated blood cells of patients with vitiligo

Effects of Ultraviolet Radiation on DNA

Genotoxic effect of simultaneous therapeutic exposure to polycyclic aromatic hydrocarbons and UV radiation

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