MICRONUCLEUS FREQUENCY AND CELL PROLIFERATION IN HUMAN LYMPHOCYTES EXPOSED TO 50 Hz SINUSOIDAL MAGNETIC FIELDS

Scarfì, Maria Rosaria; Lioi, Maria Brigida; Zeni, Olga; Noce, M. Della; Franceschi, Claudio; Bersani, Ferdinando

doi:10.1097/00004032-199903000-00005

Cited by 47 publications

(16 citation statements)

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“…The incidence of MN was not affected in cells obtained from healthy individuals and also in subjects with Turner's syndrome (TS). In a subsequent study, Scarfi et al [1999] used Helmholtz coils (80 turns of double wrapped 2 mm copper wire, 21 cm diameter) to generate sinusoidal MF. At several flux densities, the absence of an increase in MN was confirmed in cells from a larger number of healthy donors.…”

Section: Chromosomal Aberrations (Ca) Micronuclei (Mn) and Sister Cmentioning

confidence: 99%

Controversial cytogenetic observations in mammalian somatic cells exposed to extremely low frequency electromagnetic radiation: A review and future research recommendations

Vijayalaxmi

Obe

2005

Bioelectromagnetics

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During the years 1990-2003, a large number of investigations were conducted using animals, cultured rodent and human cells as well as freshly collected human blood lymphocytes to determine the genotoxic potential of exposure to nonionizing radiation emitted from extremely low frequency electromagnetic fields (EMF). Among the 63 peer reviewed scientific reports, the conclusions from 29 studies (46%) did not indicate increased damage to the genetic material, as assessed from DNA strand breaks, incidence of chromosomal aberrations (CA), micronuclei (MN), and sister chromatid exchanges (SCE), in EMF exposed cells as compared with sham exposed and/or unexposed cells, while those from 14 investigations (22%) have suggested an increase in such damage in EMF exposed cells. The observations from 20 other studies (32%) were inconclusive. This study reviews the investigations published in peer reviewed scientific journals during 1990-2003 and attempts to identify probable reason(s) for the conflicting results. Recommendations are made for future research to address some of the controversial observations.

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Section: Chromosomal Aberrations (Ca) Micronuclei (Mn) and Sister Cmentioning

confidence: 99%

Controversial cytogenetic observations in mammalian somatic cells exposed to extremely low frequency electromagnetic radiation: A review and future research recommendations

Vijayalaxmi

Obe

2005

Bioelectromagnetics

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“…At the end of the culture period (72 h growth), cells were harvested and were mounted on slides, fixed and stained with Giemsa as described elsewhere (14). After gentle homogenization of the cell suspension, 1 ml of the cell culture was centrifuged and 3 ml of lysis buffer (10 mM EDTA, 155 mM NH 4 Cl and 10 mM KHCO 3 ) was added to the cell pellet for 7 min at room temperature to break the erythrocytes.…”

Section: Cell Culture and Micronucleus Assaymentioning

confidence: 99%

Lack of Genotoxic Effects (Micronucleus Induction) in Human Lymphocytes ExposedIn Vitroto 900 MHz Electromagnetic Fields

Zeni¹,

Chiavoni²,

Sannino³

et al. 2003

Radiation Research

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In the present study, we investigated the induction of genotoxic effects in human peripheral blood lymphocytes after exposure to electromagnetic fields used in mobile communication systems (frequency 900 MHz). For this purpose, the incidence of micronuclei was evaluated by applying the cytokinesis-block micronucleus assay. Cytotoxicity was also investigated using the cytokinesis-block proliferation index. The experiments were performed on peripheral blood from 20 healthy donors, and several conditions were tested by varying the duration of exposure, the specific absorption rate (SAR), and the signal [continuous-wave (CW) or GSM (Global System of Mobile Communication) modulated signal]. The following exposures were carried out: (1) CW intermittent exposure (SAR = 1.6 W/kg) for 6 min followed by a 3-h pause (14 on/off cycles); (2) GSM signal, intermittent exposure as described in (1); (3) GSM signal, intermittent exposure as described in (1) 24 h before stimulation with phytohemagglutinin (8 on/off cycles); (4) GSM signal, intermittent exposure (SAR = 0.2 W/kg) 1 h per day for 3 days. The SARs were estimated numerically. No statistically significant differences were detected in any case in terms of either micronucleus frequency or cell cycle kinetics.

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“…Khalil and Qassem [1991], on the other hand, observed a reduction of cell proliferation in human lymphocytes exposed to pulsed EMFs (50 Hz, 1.05 mT). Scarfì et al [1999] reported an increase of cell proliferation in terms of cytokinesis block proliferation index (CBPI) in human lymphocytes exposed to ELFMFs (50 Hz 1 mT) and a decrease in cells exposed to 0.05 mT, supporting the idea that the direction of the effect can be changed according to different exposure conditions. Finally, Heredia-Rojas et al [2001] suggested that exposure to ELFMFs (60 Hz; 1.0, 1.5, and 2.0 mT) stimulates lymphocyte proliferation.…”

Section: Discussionmentioning

confidence: 83%

Absence of genotoxicity in human blood cells exposed to 50 Hz magnetic fields as assessed by comet assay, chromosome aberration, micronucleus, and sister chromatid exchange analyses

Stronati

Testa

Villani

et al. 2003

Bioelectromagnetics

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In the past, epidemiological studies indicated a possible correlation between the exposure to ELF fields and cancer. Public concern over possible hazards associated with exposure to extremely low frequency magnetic fields (ELFMFs) stimulated an increased scientific research effort. More recent research and laboratory studies, however, have not been able to definitively confirm the correlation suggested by epidemiological studies. The aim of this study was to evaluate the effects of 50 Hz magnetic fields in human blood cells exposed in vitro, using several methodological approaches for the detection of genotoxicity. Whole blood samples obtained from five donors were exposed for 2 h to 50 Hz, 1 mT uniform magnetic field generated by a Helmholtz coil system. Comet assay, sister chromatid exchanges (SCE), chromosome aberrations (CA), and micronucleus (MN) tests were used to assess DNA damage, one hallmark of malignant cell transformation. The effects of a combined exposure with X-rays were also evaluated. Results obtained do not show any significant difference between ELFMFs exposed and unexposed samples. Moreover, no synergistic effect with ionizing radiation has been observed. A slight but significant decrease of cell proliferation was evident in ELFMFs treated samples and samples subjected to the combined exposure.

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MICRONUCLEUS FREQUENCY AND CELL PROLIFERATION IN HUMAN LYMPHOCYTES EXPOSED TO 50 Hz SINUSOIDAL MAGNETIC FIELDS

Cited by 47 publications

References 0 publications

Controversial cytogenetic observations in mammalian somatic cells exposed to extremely low frequency electromagnetic radiation: A review and future research recommendations

Controversial cytogenetic observations in mammalian somatic cells exposed to extremely low frequency electromagnetic radiation: A review and future research recommendations

Lack of Genotoxic Effects (Micronucleus Induction) in Human Lymphocytes ExposedIn Vitroto 900 MHz Electromagnetic Fields

Absence of genotoxicity in human blood cells exposed to 50 Hz magnetic fields as assessed by comet assay, chromosome aberration, micronucleus, and sister chromatid exchange analyses

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