Micronucleus Test for Monitoring Genotoxicity of Polluted River Water in Rana catesbeiana Tadpoles

Wirz, M. V. M. A.; Saldiva, Paulo Hilário Nascimento; Freire-Maia, D. V.

doi:10.1007/s00128-005-0879-5

Cited by 25 publications

(8 citation statements)

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“…Micronuclei are chromosome fragments or whole chromosomes that lag at cell division due to the lack of a centromere, damage, or a defect in cytokinesis [Heddle et al, 1991]. Genotoxicity has been measured in the marine and freshwater environments by scoring MN induction in plants [Duan et al, 1999;Yang, 1999;Monarca et al, 2003], amphibians [Fernandez et al, 1989;Djomo et al, 2000;Wirz et al, 2005], mussels [Klobucar et al, 2003;Barsiene et al, 2004;Nigro et al, 2006], and fish [Ayllon and Garcia-Vazquez, 2000;De Andrade et al, 2004;Cavas and Ergene-Gozukara, 2005b;Porto et al, 2005;Da Silva Souza and Fontanetti, 2006]. Fish are extremely valuable in toxicity monitoring as they accumulate hazardous compounds in their tissues, are directly or indirectly consumed by humans, and are capable of transforming xenobiotic compounds into carcinogenic and mutagenic metabolites [Law, 2001;Van Der Oost et al, 2003].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of river water genotoxicity using the piscine micronucleus test

Ergene

Çavaş

Çelık

et al. 2007

Environ and Mol Mutagen

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The Berdan River, which empties into the Mediterranean Sea on the east coast of Turkey, receives discharges of industrial and municipal waste. In the present study, the in vivo piscine micronucleus (MN) test was used to evaluate the genotoxicity of water samples collected from different locations along the Berdan River. Nile tilapia (Oreochromis niloticus) were exposed in the laboratory for 2, 4, and 6 days, and micronuclei were evaluated in peripheral blood erythrocytes, gill cells, and caudal fin epithelial cells. A single dose of 5 mg/L cyclophosphamide was used as a positive control. In addition to micronuclei, nuclear abnormalities (NAs), such as binucleated cells and blebbed, notched, and lobed nuclei, were assessed in the erythrocytes, and chemical analyses were carried out to determine the amount of heavy metals in the water samples. MN and NA frequencies were significantly elevated (up to 2- to 3-fold) in fish exposed to river water samples taken downstream of potential discharges, and the elevated responses in gill and fin cells were related to the concentration of heavy metals in the water. MN frequencies (expressed as micronucleated cells/1,000 cells), in both treated and untreated fish, were greatest in gill cells (range: 0.80-3.70), and generally lower in erythrocytes (range: 0.50-2.80), and fin cells (range: 0.45-1.70). The results of this study indicate that the Berdan River is contaminated with genotoxic pollutants and that the genotoxicity is related to the discharge of wastes into the river water.

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Section: Introductionmentioning

confidence: 99%

Evaluation of river water genotoxicity using the piscine micronucleus test

Ergene

Çavaş

Çelık

et al. 2007

Environ and Mol Mutagen

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“…Micro nuclei test is recommended to be conducted as part of the monitoring protocols in aquatic toxicological assessment programs 26,39 . The in vivo micronuclei frequency assay has been widely used as a technique for genotoxicity monitoring of polluted aquatic media and in the screening for the presence of toxic compounds suspected to be genotoxic 29,45 . Morphological alterations in the nuclear envelope in erythrocytes of fish as described by Carrasco et al (1990) are considered indicators of genotoxic damage and constitutes a complementary analysis to the scoring of micronuclei.…”

Section: Resultsmentioning

confidence: 99%

Monitoring of Nuclear Abnormality Frequencies as Indicators of Environmental Pollution in Peripheral Erythrocytes of Labeo rohita Reared in Lakes of Bangalore

Noor

Zutshi

2018

Journal of Ecophysiology and Occupational Health

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The present study was aimed to evaluate genotoxicity in peripheral erythrocytes of Labeo rohita reared in two lakes viz., Vengaiah lake -sewage polluted (Lake A) and Yellamallappa Chetty lake -industrially polluted (Lake B) of Bangalore. To assess the micronuclei and nuclear abnormalities in such erythrocytes, blood samples were collected from heart of the freshwater fish, L. rohita anesthetized by MS222. The results were compared with the fish reared in the Hebbal fish farm (Control). The data revealed significantly high frequencies of erythrocytic abnormalities including nuclear as well as cytoplasmic in the fish blood sampled from lake B when compared to those of lake A. Such abnormalities in erythrocytes of fish varied seasonally also with summer exhibiting maximum deformities which can be attributed to the presence of genotoxic pollutants in the selected water bodies. The values were statistically significant at P<0.0001.

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“…The in vivo micronuclei frequency assay has been widely used as a technique for genotoxicity monitoring of polluted aquatic media and in the screening for the presence of toxic compounds suspected to be genotoxic (Ossana et al, 2010;Wirz et al, 2005). This test is recommended to be conducted as part of the monitoring protocols in aquatic toxicological assessment programs (Ohe et al, 2004;Udroiu, 2006); it has been proved to be a reliable index of both chromosome breakage and chromosome loss (Schmid, 1975).…”

Section: Discussionmentioning

confidence: 99%

Micronucleus test for monitoring the genotoxic potential of the surface water of Luján River (Argentina) using erythrocytes of Lithobates catesbeianus tadpoles

Ossana

Salibián

2013

Ecotoxicol. Environ. Contam.

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Luján river is a peri-urban ecosystem dominated by urban, industrial and agricultural discharges, receiving contributions from about one hundred streams along its path. The toxicological quality of the surface water in the middle course of the river was monitored by means of the micronucleus (MN) test, using peripheral erythrocytes of Lithobates catesbeianus premetamorphic tadpoles. This cytogenetic assay was performed to evaluate the effects of a particular domestic/industrial effluent poured continuously into the river, by incubating the larvae for 6 days to water samples from two sites: upstream and downstream the point where the effluent is dumped. The positive control was a cyclophosphamide solution. Five seasonal samplings were conducted between 2007 and 2008. The physicochemical analyses and the values of three water quality indices showed a highly polluted condition of the river. The MN frequency in the erythrocytes of the larvae exposed to the river samples was significantly higher than that of negative controls in all the cases and, in most samples, without differences with respect to the positive controls or even higher. There were no significant differences in the frequency of MN between the two environmental samples. The results allowed showing the genotoxic potential of the Lujan river water.

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Micronucleus Test for Monitoring Genotoxicity of Polluted River Water in Rana catesbeiana Tadpoles

Cited by 25 publications

References 0 publications

Evaluation of river water genotoxicity using the piscine micronucleus test

Evaluation of river water genotoxicity using the piscine micronucleus test

Monitoring of Nuclear Abnormality Frequencies as Indicators of Environmental Pollution in Peripheral Erythrocytes of Labeo rohita Reared in Lakes of Bangalore

Micronucleus test for monitoring the genotoxic potential of the surface water of Luján River (Argentina) using erythrocytes of Lithobates catesbeianus tadpoles

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