Background and objectives: Ancient wheat species, einkorn and emmer wheat, are potential healthy food sources for their vitamin and element contents. The aim of this study was to evaluate some elements and vitamin A and B complex of 36 einkorn and 49 emmer wheat advanced lines tested under field conditions. Findings: The results showed that some emmer wheat lines were extremely higher than those from einkorn and durum wheat checks for microelements. The mean concentrations of Fe, Zn, Cu, Mn, and Se of the emmer wheat lines were found to be 41.72 AE 3.57, 17.06 AE 0.63, 2.85 AE 0.15, 18.11 AE 0.71, and 0.05 AE 0.00 mg/kg, respectively. In addition, the means of A, B1, B2, B5, and B6 concentrations were determined as 65.48 AE 2.39 lg/kg, 4.22 AE 0.13 mg/kg, 0.36 AE 0.05 mg/kg, 3.60 AE 0.21 mg/kg, and 2.06 AE 0.09 mg/kg, respectively. Conclusions: The emmer wheat lines of 24, 43, 44, and 53 for elemental and 3, 4, 11, 17, and 24 for vitamin A and B complex can be selected for enrichment of the wheat products. Significance and novelty: Superiority of emmer wheat lines in terms of micronutrients and vitamin A and B complex compared to einkorn lines and durum wheat cultivars was obviously demonstrated. K E Y W O R D S einkorn, elements, emmer wheat, vitamins