Phosphorus plays a crucial role in regulating many of the plant’s metabolic activities by enhancing physiological functions and stimulating biological activities such as nodulation, nitrogen fixation, and nutrient uptake in the soil rhizosphere environment. Inoculants of phosphorus solubilizing bacteria serve as an eco-friendly alternative technology that positively influences both soil sustainability and plant growth. The majority of North Shewa highland areas are characterized by low available phosphorus, primarily acidic, and exhibit strong phosphorus absorption. The objective of this study was to isolate and identify phosphorus solubilizing bacteria from the rhizosphere of lentils and characterize their phosphate solubilizing activity. The cultural, biochemical, physiological microbial analysis was conducted in the microbiology laboratory, department of biology. Pikovskaya’s medium was utilized for the isolation, screening, and maintenance of phosphate solubilizing bacteria. Phosphate Solubilizing Bacteria were isolated using tri-calcium phosphate as the sole source of phosphorus in indicator plates. Fifteen phosphate solubilizing bacteria were isolated from lentil rhizosphere soil samples, among which six were the most efficient phosphate solubilizers designated as PSBYE, PSBYR, PSBYM, PSBYL, PSBW, and PSBSW. All isolates notably solubilized tri-calcium phosphate compared to the uninoculated control. The highest phosphorous solubilization was observed from the isolate PSBYL, with a value of 10.61mg/50ml, followed by PSBW with a value of 9.08 mg/50ml. The decrease in pH value correlated with the levels of tri-phosphate solubilization in the PVK broth by the PSB isolates. The pH dropped to 4.64 from the initial pH of 7.2 when grown in the broth, which suggests that the production of organic acids is likely the primary mechanism for phosphate solubilization.
