In all units where health care is provided, especially in intensive care units, infection-related complications are conditions that increase the cost and complicate the treatment process. In our study, in 2014-2015, the Ministry of Health Kartal Dr. Data of 1204 patients followed up in intensive care units of Lutfi Kirdar Training and Research Hospital were analyzed. We excluded the patients with antibiotic therapy at admission to the intensive care unit. Cases followed up in the intensive care unit for less than 24 h, and cases with culture detected in the first 48 h were also excluded from the study. Data from 287 patients with culture-positive infections of 743 patients who met the study admission criteria were analyzed. In diagnosing infectious diseases, laboratoryproven blood circulation infections were grouped as catheter-related urinary tract, central venous catheter-related bloodstream infection, ventilator-associated pneumonia, and burn infection. The hospital infections detected in our intensive care units and the determination of the causative pathogens were evaluated according to the differences in these infections according to years, units, diagnoses, demographic data, and clinical presentations. It was determined that hospitalization diagnoses, presence of comorbid diseases, and the percentage of burns were firmly related to the duration of ICU stay and mortality. Acinetobacter baumannii infections were significantly higher in our intensive care units compared with other microorganisms. We aimed to evaluate the data of the patients who were followed up in intensive care units of Dr. Lutfi Kirdar Kartal Training and Research Hospital between 2014 and 2015 with culture-positive infection retrospectively.