Microorganisms of kaolins and their role in the processes of iron solubilization and transformation

Shelobolina, Evgenya S.; Parfenova, E.Yu.; Avakyan, Z. A.

doi:10.1016/s1572-4409(99)80057-1

Cited by 8 publications

(6 citation statements)

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“…The bottle headspace was gassed with N 2 . Microbial enrichments were established at the Institute of Microbiology of the Russian Academy of Sciences as part of a study into the role of micro-organisms within kaolins in the processes of iron solubilization and transformation (Shelobolina et al, 1999).…”

Section: Strain R7mentioning

confidence: 99%

“…+, Positive; 2, negative; ND, not determined. (Shelobolina et al, 1999(Shelobolina et al, , 2005 as well as humic and fulvic acids (Elzea Kogel et al, 2002). At the same time, kaolins are characterized by low amounts of 'bioavailable' Fe(III) (0.5 M HCl extractable hydroxylamine reducable; 0.0-0.9 mmol kg 21 ) compared with total Fe(III) (hydrofluoric acid-extractable, 7.7-48.6 mmol kg 21 ) (Shelobolina et al, 2005).…”

Section: Potential Role Of the Novel Isolates In Iron Cycling In Kaolin Stratamentioning

confidence: 99%

“…The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of Geobacter pickeringii G13 Subsurface kaolin lenses are not optimal environments for microbial growth due to the fine mineral particle size of clay and consequently the low hydraulic conductivity. However, cultivation-dependent most probable number analysis (Shelobolina et al, 1999(Shelobolina et al, , 2005 and cultivation-independent lipid biomarker analysis (Osipov & Turova, 1997) have shown the presence of a variety of physiological groups of micro-organisms in kaolin samples of differing ages and origins collected from distant geographical locations in Russia, Ukraine and the USA. Stimulation of indigenous iron-reducing bacteria in kaolin results in a decrease of the Fe(III) : [Fe(II)+Fe(III)] ratio (Shelobolina et al, 1999(Shelobolina et al, , 2005, iron solubilization (Turova et al, 1996;Lee et al, 1999;Shelobolina et al, 1999) and in a dramatic increase in the efficacy of iron extraction by magnetic separation (Turova et al, 1996;Vodyanitskii et al, 1997;Shelobolina et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…However, cultivation-dependent most probable number analysis (Shelobolina et al, 1999(Shelobolina et al, , 2005 and cultivation-independent lipid biomarker analysis (Osipov & Turova, 1997) have shown the presence of a variety of physiological groups of micro-organisms in kaolin samples of differing ages and origins collected from distant geographical locations in Russia, Ukraine and the USA. Stimulation of indigenous iron-reducing bacteria in kaolin results in a decrease of the Fe(III) : [Fe(II)+Fe(III)] ratio (Shelobolina et al, 1999(Shelobolina et al, , 2005, iron solubilization (Turova et al, 1996;Lee et al, 1999;Shelobolina et al, 1999) and in a dramatic increase in the efficacy of iron extraction by magnetic separation (Turova et al, 1996;Vodyanitskii et al, 1997;Shelobolina et al, 1999). The ability of indigenous bacteria to influence iron mineralogy in kaolin suggests that bacteria have played a significant role in the natural removal of iron impurities from kaolin at the subsurface (Hurst & Pickering, 1997;Elzea Kogel et al, 2002;Shelobolina et al, 2005) and can be used for the development of microbially mediated industrial-scale iron removal from tinted kaolin (Avakyan et al, 1997;Lee et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

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Geobacter pickeringii sp. nov., Geobacter argillaceus sp. nov. and Pelosinus fermentans gen. nov., sp. nov., isolated from subsurface kaolin lenses

Shelobolina

Nevin

Blakeney-Hayward

et al. 2007

International Journal of Systematic and Evolutionary Microbiology

133

105

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The goal of this project was to isolate representative Fe(III)-reducing bacteria from kaolin clays that may influence iron mineralogy in kaolin. Two novel dissimilatory Fe(III)-reducing bacteria, strains G12T and G13T, were isolated from sedimentary kaolin strata in Georgia (USA). Cells of strains G12T and G13T were motile, non-spore-forming regular rods, 1–2 μm long and 0.6 μm in diameter. Cells had one lateral flagellum. Phylogenetic analyses using the 16S rRNA gene sequence of the novel strains demonstrated their affiliation to the genus Geobacter. Strain G12T was most closely related to Geobacter pelophilus (94.7 %) and Geobacter chapellei (94.1 %). Strain G13T was most closely related to Geobacter grbiciae (95.3 %) and Geobacter metallireducens (95.1 %). Based on phylogenetic analyses and phenotypic differences between the novel isolates and other closely related species of the genus Geobacter, the isolates are proposed as representing two novel species, Geobacter argillaceus sp. nov. (type strain G12T=ATCC BAA-1139T=JCM 12999T) and Geobacter pickeringii sp. nov. (type strain G13T=ATCC BAA-1140T=DSM 17153T=JCM 13000T). Another isolate, strain R7T, was derived from a primary kaolin deposit in Russia. The cells of strain R7T were motile, spore-forming, slightly curved rods, 0.6×2.0–6.0 μm in size and with up to six peritrichous flagella. Strain R7T was capable of reducing Fe(III) only in the presence of a fermentable substrate. 16S rRNA gene sequence analysis demonstrated that this isolate is unique, showing less than 92 % similarity to bacteria of the Sporomusa–Pectinatus–Selenomomas phyletic group, including ‘Anaerospora hongkongensis’ (90.2 %), Acetonema longum (90.6 %), Dendrosporobacter quercicolus (90.9 %) and Anaerosinus glycerini (91.5 %). On the basis of phylogenetic analysis and physiological tests, strain R7T is proposed to represent a novel genus and species, Pelosinus fermentans gen. nov., sp. nov. (type strain R7T=DSM 17108T=ATCC BAA-1133T), in the Sporomusa–Pectinatus–Selenomonas group.

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Section: Strain R7mentioning

confidence: 99%

Section: Potential Role Of the Novel Isolates In Iron Cycling In Kaolin Stratamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Geobacter pickeringii sp. nov., Geobacter argillaceus sp. nov. and Pelosinus fermentans gen. nov., sp. nov., isolated from subsurface kaolin lenses

Shelobolina

Nevin

Blakeney-Hayward

et al. 2007

International Journal of Systematic and Evolutionary Microbiology

133

105

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“…The high numbers of potentially viable microbial contaminants (also found in previous studies by other authors, e.g. [59] , indicate that fillers of polymers should not be dismissed in studies of encapsulated bioburden.…”

Section: Discussionmentioning

confidence: 68%

Quantification of Encapsulated Bioburden in Spacecraft Polymer Materials by Cultivation-Dependent and Molecular Methods

Bauermeister¹,

Mahnert

Auerbach

et al. 2014

PLoS ONE

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Bioburden encapsulated in spacecraft polymers (such as adhesives and coatings) poses a potential risk to jeopardize scientific exploration of other celestial bodies. This is particularly critical for spacecraft components intended for hard landing. So far, it remained unclear if polymers are indeed a source of microbial contamination. In addition, data with respect to survival of microbes during the embedding/polymerization process are sparse. In this study we developed testing strategies to quantitatively examine encapsulated bioburden in five different polymers used frequently and in large quantities on spaceflight hardware. As quantitative extraction of the bioburden from polymerized (solid) materials did not prove feasible, contaminants were extracted from uncured precursors. Cultivation-based analyses revealed <0.1–2.5 colony forming units (cfu) per cm3 polymer, whereas quantitative PCR-based detection of contaminants indicated considerably higher values, despite low DNA extraction efficiency. Results obtained from this approach reflect the most conservative proxy for encapsulated bioburden, as they give the maximum bioburden of the polymers irrespective of any additional physical and chemical stress occurring during polymerization. To address the latter issue, we deployed an embedding model to elucidate and monitor the physiological status of embedded Bacillus safensis spores in a cured polymer. Staining approaches using AlexaFluor succinimidyl ester 488 (AF488), propidium monoazide (PMA), CTC (5-cyano-2,3-diotolyl tetrazolium chloride) demonstrated that embedded spores retained integrity, germination and cultivation ability even after polymerization of the adhesive Scotch-Weld 2216 B/A. Using the methods presented here, we were able to estimate the worst case contribution of encapsulated bioburden in different polymers to the bioburden of spacecraft. We demonstrated that spores were not affected by polymerization processes. Besides Planetary Protection considerations, our results could prove useful for the manufacturing of food packaging, pharmacy industry and implant technology.

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PCR-DGGE analysis of the bacterial composition of a kaolin slurry showing altered rheology

Papp

Balázs

Tombácz

et al. 2011

World J Microbiol Biotechnol

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Kaolin is an important industrial raw material and a basis of a range of different products. Microbial spoilage is a detrimental process observed especially in kaolin slurries, leading to low quality products and economic loss. Although the alteration of kaolin slurries in ceramic industry was observed, the process and the microbial background have not been analyzed in details. This study provides the first data using a cultivation independent molecular biological approach (PCR-DGGE) regarding the bacterial composition of an altered kaolin slurry. The results show that potential exopolymer (EPS) producer bacteria (e.g. Acinetobacter, Pseudomonas) appear in the altered kaolin slurry, which may have an important role in the modification of kaolin slurries.

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Microorganisms of kaolins and their role in the processes of iron solubilization and transformation

Cited by 8 publications

References 0 publications

Geobacter pickeringii sp. nov., Geobacter argillaceus sp. nov. and Pelosinus fermentans gen. nov., sp. nov., isolated from subsurface kaolin lenses

Geobacter pickeringii sp. nov., Geobacter argillaceus sp. nov. and Pelosinus fermentans gen. nov., sp. nov., isolated from subsurface kaolin lenses

Quantification of Encapsulated Bioburden in Spacecraft Polymer Materials by Cultivation-Dependent and Molecular Methods

PCR-DGGE analysis of the bacterial composition of a kaolin slurry showing altered rheology

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