Microparticle-loaded neonatal porcine Sertoli cells for cell-based therapeutic and drug delivery system

Giovagnoli, Stefano; Mancuso, Francesca; Vannini, Samuele; Calvitti, Mario; Piroddi, Marta; Pietrella, Donatella; Arato, Iva; Falabella, G.; Galli, Francesco; Moretti, Massimo; Neri, Luca M.; Bodo, Maria; Capitani, Silvano; Cameron, Don F.; Ricci, Maurizio; Luca, Giovanni; Calafiore, Riccardo

doi:10.1016/j.jconrel.2014.08.001

Cited by 14 publications

(16 citation statements)

References 49 publications

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“…Aliquots of the culture media of treated and untreated SCs were collected and stored at −20 °C for the assessment of AMH (AMH Gen II ELISA, Beckman Coulter, Webster, TX, USA) (intra-assay CV = 3.9%; inter-assay CV = 5.8%) and inhibin B (inhibin B Gen II ELISA, Beckman Coulter, Webster, TX, USA) (intra-assay CV = 2.8%; inter-assay CV = 4.3%) concentrations as previously described [19].…”

Section: Methodsmentioning

confidence: 99%

Effects of Insulin on Porcine Neonatal Sertoli Cell Responsiveness to FSH In Vitro

Cannarella

Arato

Condorelli

et al. 2019

JCM

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There is ongoing debate as to whether the decline of sperm production in recent times may be related to a parallel increase in the rate of obesity and diabetes. Lower anti-Müllerian hormone (AMH) and inhibin B secretion have been observed in young hyperinsulinemic patients compared to healthy controls, suggesting a Sertoli cell (SC) dysfunction. The pathophysiological mechanisms underlying SC dysfunction in these patients are poorly understood. To the best of our knowledge, no evidence is available on the effects of insulin on SC function. Therefore, this study was undertaken to assess the effects of insulin on basal and follicle-stimulating hormone (FSH)-stimulated SC function in vitro. To accomplish this, we evaluated the expression of AMH, inhibin B and FSHR genes, the secretion of AMH and inhibin B and the phosphorylation of AKT473 and SC proliferation on neonatal porcine SC after incubation with FSH and/or insulin. We found that similar to FSH, the expression and secretion of AMH is suppressed by insulin. Co-incubation with FSH and insulin decreased AMH secretion significantly more than with FSH alone. Insulin had no effect on the expression and secretion of the inhibin B gene, but co-incubation with FSH and insulin had a lower effect on inhibin B secretion than that found with FSH alone. FSH and/or insulin increased AKT473 phosphorylation and SC proliferation. In conclusion, the results of this study showed that insulin modulates SC function. We hypothesize that hyperinsulinemia may therefore influence testicular function even before puberty begins. Therefore, particular care should be taken to avoid the onset of hyperinsulinemia in children to prevent a future deleterious effect on fertility.

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Section: Methodsmentioning

confidence: 99%

Effects of Insulin on Porcine Neonatal Sertoli Cell Responsiveness to FSH In Vitro

Cannarella

Arato

Condorelli

et al. 2019

JCM

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“…Upon animal sacrifice, fragments of muscle and liver were weighted and suspended in the appropriate volume of radioimmunoprecipitation assay (RIPA) buffer . The samples were homogenized by UltraTurrax (Janke & Kunkel, Staufen, Deutschland), in ice, until fragments were no longer visible.…”

Section: Methodsmentioning

confidence: 99%

“…Real‐time PCR [for GLUT‐4, GLUT‐2, GK, inducible NOS (iNOS), IL‐6, uncoupling protein (UCP‐1)] analyses were conducted as previously described, using the primers listed in Table . Briefly, total RNA was isolated, from samples obtained in the three different experimental groups, by RNA purification kit (Versagene RNA Cell Kit, Gentra Systems, Minneapolis, MN, USA) and quantified by reading the optical density at 260 nm.…”

Section: Methodsmentioning

confidence: 99%

Xenograft of microencapsulated Sertoli cells restores glucose homeostasis in db/db mice with spontaneous diabetes mellitus

Luca

Arato

Mancuso

et al. 2016

Xenotransplantation

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“…Aliquots of the culture media of treated and untreated SCs were stored at −20 °C for the assessment of AMH (AMH Gen IIELISA, Beckman Coulter; intra-assay CV = 3.89%; inter-assay CV = 5.77%) and inhibin B (Inhibin B Gen II ELISA, Beckman Coulter, Webster, TX, USA; intra-assay CV = 2.81%; inter-assay CV = 4.33%) secretion, as previously described [12].…”

Section: Methodsmentioning

confidence: 99%

Effects of GH and IGF1 on Basal and FSH-Modulated Porcine Sertoli Cells In-Vitro

Cannarella

Mancuso

Condorelli

et al. 2019

JCM

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Several lines of evidence suggest that insulin-like growth factor 1 (IGF1) is involved in Sertoli cell (SC) proliferation and that its receptor (IGF1R) could mediate follicle-stimulating hormone (FSH) effects. To examine the role of the growth hormone (GH)-IGF1 axis on SC function, we evaluated the effects of GH and IGF1 on basal and FSH-modulated SC proliferation, as well as on anti-Müllerian hormone (AMH) and inhibin B expression and secretion in-vitro. SCs from neonatal pigs were incubated with (1) placebo, (2) 100 nM highly purified urofollitropin (hpFSH), (3) 100 nM recombinant GH (rGH), (4) 100 nM recombinant IGF1 (rIGF1), (5) 100 nM hpFSH plus 100 nM rGH, (6) 100 nM hpFSH plus 100 nM rIGF1, for 48 h. We found that IGF1, but not FSH nor GH, stimulated SC proliferation. Furthermore, an inhibitory effect of FSH, GH and IGF1 on AMH secretion, and a stimulatory role of FSH and IGF1, but not GH, on inhibin B secretion were found. These results suggest that the GH-IGF1 axis influences basal and FSH-modulated SC proliferation and function. We speculate that SC proliferation occurring in childhood might be supported by the increased serum IGF1 levels observed during this period of life.

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Microparticle-loaded neonatal porcine Sertoli cells for cell-based therapeutic and drug delivery system

Cited by 14 publications

References 49 publications

Effects of Insulin on Porcine Neonatal Sertoli Cell Responsiveness to FSH In Vitro

Effects of Insulin on Porcine Neonatal Sertoli Cell Responsiveness to FSH In Vitro

Xenograft of microencapsulated Sertoli cells restores glucose homeostasis in db/db mice with spontaneous diabetes mellitus

Effects of GH and IGF1 on Basal and FSH-Modulated Porcine Sertoli Cells In-Vitro

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