Microparticles-Mediated Vascular Inflammation and its Amelioration by Antioxidant Activity of Baicalin

Paudel, Keshav Raj; Kim, Dong-Wook

doi:10.3390/antiox9090890

Cited by 35 publications

(17 citation statements)

References 51 publications

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“…When administered in vivo to a mouse MI model, these EVs preserved ventricular function and angiogenesis and decreased the degree of fibrosis in the infarct area [ 227 ]. In a study of vascular inflammatory mediators, EVs from LPS-treated endothelial cells stimulated endothelial dysfunction as shown by increased production of ICAM protein, ROS, and onset of cellular senescence but decreased levels of phospho-endothelial nitric oxide synthase and pAKT, while EVs from vascular smooth muscle cells produced increased levels of anti-fibrotic MMP-9 [ 228 ].…”

Section: Cardiac Fibrosismentioning

confidence: 99%

Extracellular Vesicles in Organ Fibrosis: Mechanisms, Therapies, and Diagnostics

Brigstock

2021

Cells

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Fibrosis is the unrelenting deposition of excessively large amounts of insoluble interstitial collagen due to profound matrigenic activities of wound-associated myofibroblasts during chronic injury in diverse tissues and organs. It is a highly debilitating pathology that affects millions of people globally and leads to decreased function of vital organs and increased risk of cancer and end-stage organ disease. Extracellular vesicles (EVs) produced within the chronic wound environment have emerged as important vehicles for conveying pro-fibrotic signals between many of the cell types involved in driving the fibrotic response. On the other hand, EVs from sources such as stem cells, uninjured parenchymal cells, and circulation have in vitro and in vivo anti-fibrotic activities that have provided novel and much-needed therapeutic options. Finally, EVs in body fluids of fibrotic individuals contain cargo components that may have utility as fibrosis biomarkers, which could circumvent current obstacles to fibrosis measurement in the clinic, allowing fibrosis stage, progression, or regression to be determined in a manner that is accurate, safe, minimally-invasive, and conducive to repetitive testing. This review highlights the rapid and recent progress in our understanding of EV-mediated fibrotic pathogenesis, anti-fibrotic therapy, and fibrosis staging in the lung, kidney, heart, liver, pancreas, and skin.

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Section: Cardiac Fibrosismentioning

confidence: 99%

Extracellular Vesicles in Organ Fibrosis: Mechanisms, Therapies, and Diagnostics

Brigstock

2021

Cells

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“…Another key factor in lung cancer progression is the significance of matrix metalloproteinases (MMPs). MMPs are proteolytic enzymes which degrade various extracellular matrix (ECM) components (Paudel & Kim, 2020). One of the major MMPs involved in lung cancer is the MMP‐9.…”

Section: Introductionmentioning

confidence: 99%

“…MMPs are proteolytic enzymes which degrade various extracellular matrix (ECM) components (Paudel & Kim, 2020). One of the major MMPs involved in lung cancer is the MMP-9.…”

Section: Introductionmentioning

confidence: 99%

Anti‐inflammatory and anticancer activities of Naringenin‐loaded liquid crystalline nanoparticles in vitro

et al. 2020

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In this study, we had developed Naringenin-loaded liquid crystalline nanoparticles (LCNs) and investigated the anti-inflammatory and anticancer activities of Naringenin-LCNs against human airway epithelium-derived basal cells (BCi-NS1.1) and human lung epithelial carcinoma (A549) cell lines, respectively. The anti-inflammatory potential of Naringenin-LCNs evaluated by qPCR revealed a decreased expression of IL-6, IL-8, IL-1β, and TNF-α in lipopolysaccharide-induced BCi-NS1.1 cells. The activity of LCNs was comparable to the positive control drug Fluticasone propionate (10 nM). The anticancer activity was studied by evaluating the antiproliferative (MTT and trypan blue assays), antimigratory (scratch wound healing assay, modified Boyden chamber assay, and immunoblot), and anticolony formation activity in A549 cells. Naringenin LCNs showed promising antiproliferative, antimigratory, and anticolony formation activities in A549 cells, in vitro. Therefore, based on our observations and results, we conclude that Naringenin-LCNs may be employed as a potential therapy-based intervention to ameliorate airway inflammation and to inhibit the progression of lung cancer. Practical applications Naringenin was encapsulated into liquid crystalline nanoparticles, thus, attributing to their sustained-release nature. In addition, Naringenin-loaded LCNs efficiently reduced the levels of pro-inflammatory markers, namely, IL-1β, IL-6, TNF-α, and IL-8. In addition, the Naringenin-loaded LCNs also possess potent anticancer activity, when tested in the A549 cell line, as revealed by the inhibition of proliferation and migration of cells. They also attenuated colony formation and induced apoptosis in the A549 cells. The findings from our study could form the basis for future research that may be translated into an in vivo model to validate the possible therapeutic alternative for lung cancer using Naringenin-loaded LCNs. In addition, the applications of

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“…Baicalin (7-glucuronic acid-5,6-dihydroxy-flavone), is one of the primary bioactive flavonoid compounds extracted from the roots of Scutellaria baicalensis Georgi. Baicalin is used clinically in humans and animals because of its antimicrobial, anti-inflammatory, antitumor and antioxidant properties [ 21 , 22 , 23 , 24 ]. Our previously work demonstrated that baicalin has anti–inflammatory effects in G. parasuis -challenged piglets in vivo and in vitro by suppressing inflammatory cytokines and HMGB1 via NF-κB and NLRP3 signaling and reversing apoptosis by altering PKC–MAPK signaling cells [ 25 , 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Baicalin on Peritoneal Tight Junctions in Piglets Challenged with Glaesserella parasuis

Zhang

et al. 2021

Molecules

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Glaesserella parasuis (G. parasuis) causes inflammation and damage to piglets. Whether polyserositis caused by G. parasuis is due to tight junctions damage and the protective effect of baicalin on it have not been examined. Therefore, this study aims to investigate the effects of baicalin on peritoneal tight junctions of piglets challenged with G. parasuis and its underlying molecular mechanisms. Piglets were challenged with G. parasuis and treated with or without baicalin. RT-PCR was performed to examine the expression of peritoneal tight junctions genes. Immunofluorescence was carried out to detect the distribution patterns of tight junctions proteins. Western blot assays were carried out to determine the involved signaling pathways. Our data showed that G. parasuis infection can down-regulate the tight junctions expression and disrupt the distribution of tight junctions proteins. Baicalin can alleviate the down-regulation of tight junctions mRNA in peritoneum, prevent the abnormalities and maintain the continuous organization of tight junctions. Our results provide novel evidence to support that baicalin has the capacity to protect peritoneal tight junctions from G. parasuis-induced inflammation. The protective mechanisms of baicalin could be associated with inhibition of the activation of PKC and MLCK/MLC signaling pathway. Taken together, these data demonstrated that baicalin is a promising natural agent for the prevention and treatment of G. parasuis infection.

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Microparticles-Mediated Vascular Inflammation and its Amelioration by Antioxidant Activity of Baicalin

Cited by 35 publications

References 51 publications

Extracellular Vesicles in Organ Fibrosis: Mechanisms, Therapies, and Diagnostics

Extracellular Vesicles in Organ Fibrosis: Mechanisms, Therapies, and Diagnostics

Anti‐inflammatory and anticancer activities of Naringenin‐loaded liquid crystalline nanoparticles in vitro

Protective Effects of Baicalin on Peritoneal Tight Junctions in Piglets Challenged with Glaesserella parasuis

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