Microparticulated Polygonatum sibiricum polysaccharide shows potent vaccine adjuvant effect

Surface-Engineered Polygonatum Sibiricum Polysaccharide CaCO3 Microparticles as Novel Vaccine Adjuvants to Enhance Immune Response

“…82 In addition, PSP, which acts as an immunostimulant, can promotes DCs activation and cytokine secretion via the TLR4 pathway. 31 Similar to previous studies, PEI−PSP-CaCO 3 induced the activation of DCs and secretion of cytokines and effectively promoted the transport of activated DCs to dLNs. The activation of T cells and germinal center B cells in dLNs was observed in the PEI−PSP-CaCO 3 -immunized mice, which may be associated with the high levels of antibodies.…”

“…A large particle size of particles can induce an antigen depot at the injection site, promoting the activation of DCs and the transport of antigens to dLNs . In addition, PSP, which acts as an immunostimulant, can promotes DCs activation and cytokine secretion via the TLR4 pathway . Similar to previous studies, PEI–PSP-CaCO 3 induced the activation of DCs and secretion of cytokines and effectively promoted the transport of activated DCs to dLNs.…”

“…In a previous study, lentinan-CaCO 3 microspheres enhanced the Th1 immune response, and we observed a Th2-dependent immune response induced by PEI−PSP-CaCO 3 and CTAB-PSP-CaCO 3 . 31 This demonstrated that PSP-CaCO 3 generated a strong cellular immune response, and PEI−PSP-CaCO 3 and CTAB-PSP-CaCO 3 showed stronger effects.…”

“…29,30 A previous study showed that PSP modified with CaCO 3 microparticles elicited a higher immune response compared with Alum adjuvant. 31 In our previous study, lentinan-modified CaCO 3 microparticles were generally negatively charged, 32 limiting their surface antigen adsorption and cellular uptake. Cationic polymer polyethylenimine (PEI) has been widely studied for coating nanoparticles and microparticles to develop positively charged particles.…”

“…They can protect biological substances from the external environment and allow their sustained release . Calcium carbonate (CaCO 3 ), one of the most abundant inorganic minerals in nature, has been widely applied in various fields (e.g., paper industry, biomedicine, cosmetics) due to its simple chemical composition, low cost, excellent biocompatibility, and biodegradability. − In recent years, CaCO 3 has been extensively studied for its use in drug delivery system due to its advantages (high porosity, good biocompatibility, easy preparation process, and low cost), which has high drug-loading capacity and good sustainable release. − CaCO 3 successfully loaded antigens and efficiently activated dendritic cells and lymphocytes, inducing strong cellular and humoral immune responses. , A previous study showed that PSP modified with CaCO 3 microparticles elicited a higher immune response compared with Alum adjuvant . In our previous study, lentinan-modified CaCO 3 microparticles were generally negatively charged, limiting their surface antigen adsorption and cellular uptake.…”