Micropatterned immobilization of membrane-mimicking polymer and peptides for regulation of cell behaviors<i>in vitro</i>

Ma, Wenyong; Liu, Luying; Chen, Huiqing; Zhao, Yuancong; Yang, Ping; Huang, Nan

doi:10.1039/c8ra02607f

Cited by 9 publications

(2 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The moderately strong peaks at 2440, 2540, and 2640 cm –1 are associated with the −NH stretching vibration in HCl-DA. The broad absorption peak around 3420 cm –1 and the absorption peak at 1640 cm –1 in the FTIR spectrum of PDA-Ga are attributed to the aromatic −NH, −NH 2 , O–H, and CN stretching vibrations, respectively, − indicating the successful coating of PDA on the surface of the Ga nanodroplets using HCl-DA. In addition, it can also be observed from Figure b that the nanodroplets modified by PDA had an increased particle size and a uniform distribution following the particle size distribution map shown in Figure d.…”

Section: Resultsmentioning

confidence: 99%

Smart Adhesive Patches of Antibacterial Performance Based on Polydopamine-Modified Ga Liquid Metal Nanodroplets

Sun

et al. 2022

ACS Appl. Nano Mater.

View full text Add to dashboard Cite

Patches have been widely used in medical and healthcare, motion detection, and other fields. Patches are required to possess strong adhesive forces to keep them effectively adhered to the skin during use and to be removed from the skin with relative ease after usage. Moreover, a large number of bacteria will proliferate at the interface between the patch and the skin, when the patch is used for lengthy durations, which is harmful to human health. Hence, there is an increasing demand for patches with combined switchable adhesions and excellent antibacterial performances. Inspired by the natural adhesive performance of snail slime in wet (liquid) and dry (solid) states, smart Ga liquid metal nanodroplet-based polymer patch (GxPP, x = 10, 30, 50) with switchable adhesion properties were prepared by incorporating polydimethylsiloxane (PDMS) into polydopamine (PDA)-modified Ga nanodroplets (PDA-Ga). Since the melting point of Ga is 29.8 °C, solid Ga melts to the liquid state at temperatures above 29.8 °C, and this significantly decreases the modulus of GxPP, resulting in significantly decreased adhesive strength of GxPP. The adhesion of G30PP decreased from 40.4 to 22.1 kPa. In addition to this obvious change in the adhesion properties of GxPP, the unique antibacterial performances of Ga imparted GxPP with excellent antibacterial effects. Compared with the PDMS, the antibacterial rates of G30PP against Escherichia coli and Staphylococcus aureus were 98 and 99%, respectively. The excellent antibacterial performance and smart switchable adhesion properties of GxPP revealed its high application prospects in the field of wearable flexible skin electronics.

show abstract

Section: Resultsmentioning

confidence: 99%

Smart Adhesive Patches of Antibacterial Performance Based on Polydopamine-Modified Ga Liquid Metal Nanodroplets

Sun

et al. 2022

ACS Appl. Nano Mater.

View full text Add to dashboard Cite

show abstract

“…Therefore, the site-specific mutagenesis of butanol dehydrogenase ( butA ) gene might be further improved the AC and 2,3-BD yield and selectivity in C. creantum . In addition, although the repeated batch biocatalysis showed a high average productivity for AC and 2,3-BD production, the catalytic efficiency significantly deceased after only two batches, which can be further improved by cell immobilization to increase the cell viability and stability [44, 45]. On the other hand, process engineering, including buffer optimization, substrate concentration optimization, multiple biocatalysis strategies, and high cell density et al, can further improve AC and 2,3-BD production for commercial development.…”

Section: Resultsmentioning

confidence: 99%

Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method

et al. 2019

View full text Add to dashboard Cite

Background: Acetoin (AC) and 2,3-butanediol (2,3-BD) as highly promising bio-based platform chemicals have received more attentions due to their wide range of applications. However, the non-efficient substrate conversion and mutually transition between AC and 2,3-BD in their natural producing strains not only led to a low selectivity but also increase the difficulty of downstream purification. Therefore, synthetic engineering of more suitable strains should be a reliable strategy to selectively produce AC and 2,3-BD, respectively. Results: In this study, the respective AC (alsS and alsD) and 2,3-BD biosynthesis pathway genes (alsS, alsD, and bdhA) derived from Bacillus subtilis 168 were successfully expressed in non-natural AC and 2,3-BD producing Corynebacterium crenatum, and generated recombinant strains, C. crenatum SD and C. crenatum SDA, were proved to produce 9.86 g L −1 of AC and 17.08 g L −1 of 2,3-BD, respectively. To further increase AC and 2,3-BD selectivity, the AC reducing gene (butA) and lactic acid dehydrogenase gene (ldh) in C. crenatum were then deleted. Finally, C. crenatumΔbutAΔldh SD produced 76.93 g L −1 AC in one-step biocatalysis with the yield of 0.67 mol mol −1. Meanwhile, after eliminating the lactic acid production and enhancing 2,3-butanediol dehydrogenase activity, C. crenatumΔldh SDA synthesized 88.83 g L −1 of 2,3-BD with the yield of 0.80 mol mol −1. Conclusions: The synthetically engineered C. crenatumΔbutAΔldh SD and C. crenatumΔldh SDA in this study were proved as an efficient microbial cell factory for selective AC and 2,3-BD production. Based on the insights from this study, further synthetic engineering of C. crenatum for AC and 2,3-BD production is suggested.

show abstract

Robust aerogel of two-dimensional composite microparticle of amorphous ruthenium-cobalt hydroxide and GO for hydrogen generation via NaBH4 hydrolysis

Li,

Wang,

Wang

et al. 2024

International Journal of Hydrogen Energy

View full text Add to dashboard Cite

Micropatterned immobilization of membrane-mimicking polymer and peptides for regulation of cell behaviorsin vitro

Abstract: The Ti-PDA-M/R(P) biomimetic micropattern was successfully fabricated with PMMPC-HD and GREDVY. The Ti-PDA-M/R(P) micropattern can regulate EC morphology, orientation and functions, and inhibit platelet adhesion and proliferation of SMCs.

Cited by 9 publications

References 60 publications

Smart Adhesive Patches of Antibacterial Performance Based on Polydopamine-Modified Ga Liquid Metal Nanodroplets

Smart Adhesive Patches of Antibacterial Performance Based on Polydopamine-Modified Ga Liquid Metal Nanodroplets

Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method

Robust aerogel of two-dimensional composite microparticle of amorphous ruthenium-cobalt hydroxide and GO for hydrogen generation via NaBH4 hydrolysis

Contact Info

Product

Resources

About