2019
DOI: 10.1002/bit.27075
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Micropatterned substrates with physiological stiffness promote cell maturation and Pompe disease phenotype in human induced pluripotent stem cell‐derived skeletal myocytes

Abstract: Recent advances in bioengineering have enabled cell culture systems that more closely mimic the native cellular environment. Here, we demonstrated that human induced pluripotent stem cell (iPSC)-derived myogenic progenitors formed highlyaligned myotubes and contracted when seeded on two-dimensional micropatterned platforms. The differentiated cells showed clear nuclear alignment and formed elongated myotubes dependent on the width of the micropatterned lanes.

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Cited by 35 publications
(18 citation statements)
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“…The increase production of extracellular matrix components such as GAG is important for when trying to regenerate corneal stroma either by replacing a scaffold over time or by using a self-assembly approach, where the cells need to produce sufficient ECM in culture to form a functional tissue 44 . Micro-patterns on a substrate surface and substrate stiffness can induce cues to improve formation and alignment of multinucleated myotubes, similar to myofibers,that can exhibit spontaneous contractions along the pattern's longitudinal direction 45 .…”
Section: Discussionmentioning
confidence: 99%
“…The increase production of extracellular matrix components such as GAG is important for when trying to regenerate corneal stroma either by replacing a scaffold over time or by using a self-assembly approach, where the cells need to produce sufficient ECM in culture to form a functional tissue 44 . Micro-patterns on a substrate surface and substrate stiffness can induce cues to improve formation and alignment of multinucleated myotubes, similar to myofibers,that can exhibit spontaneous contractions along the pattern's longitudinal direction 45 .…”
Section: Discussionmentioning
confidence: 99%
“…Alternatively, photolithography and/or soft lithography are also applied as micropatterning techniques to produce various surface topographies. In one study, the cultivation of human iPSC-derived myogenic progenitors on PDMS-based micropatterned substrate resulted in larger fiber diameters, more myogenin-positive nuclei, and increased nuclear fusion compared to the cells cultivated on non-patterned substrates [ 209 ]. Freeze-drying is another technique used for microstructured scaffolds in skeletal muscle tissue engineering.…”
Section: Scaffold Topographies For Improved Generation Of Muscle Tissuementioning
confidence: 99%
“…For example, PDMS stamps generated using the same photolithography techniques described above can be used to transfer ECM proteins onto a surface in a process known as microcontact printing ( Qin et al, 2010 ). This process has been used to prescribe myotube alignment on Petri dishes ( Bajaj et al, 2011 ), PDMS-coated surfaces ( Bettadapur et al, 2016 ; Jiwlawat et al, 2019 ; Nesmith et al, 2016 ; Palchesko et al, 2012 ; Sun et al, 2013 ) and PA hydrogels ( Li et al, 2008a ). Photolithography has also been used to selectively expose strips of a PA hydrogel to UV light, which activates only the exposed regions for collagen binding and thus myoblast adhesion ( Engler et al, 2004 ).…”
Section: Engineered In Vitro Models Of Neuromusculmentioning
confidence: 99%