Micropillar enhanced FRET-CRISPR biosensor for nucleic acid detection

Bao, Mengdi; Dollery, Stephen J.; Yuqing, FNU; Tobin, Gregory J.; Du, Ke

doi:10.1039/d3lc00780d

Lab Chip

2024

DOI: 10.1039/d3lc00780d

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Micropillar enhanced FRET-CRISPR biosensor for nucleic acid detection

Mengdi Bao,

Stephen J. Dollery,

FNU Yuqing

et al.

Abstract: High-aspect ratio micropillar arrays are fabricated to enhance the detection performance of FRET-CRISPR assay.

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2024

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Cited by 5 publications

References 48 publications

(65 reference statements)

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Micropillars in Cell Mechanobiology: Design, Fabrication, Characterization, and Biosensing Applications

De Saram,

Nguyen,

Jamali

et al. 2024

Small Science

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Eukaryotic cells possess the remarkable ability to sense and respond to mechanical cues from their extracellular environment, a phenomenon known as mechanobiology, which is crucial for the proper functioning of biological systems. Micropillars have emerged as a prominent tool for quantifying cellular forces and have demonstrated versatility beyond force measurement, including the modulation of the extracellular environment and the facilitation of mechano‐stimulation. In this comprehensive review, innovative strategies in micropillars’ design, fabrication, characterization, and biosensing applications are explored. The review begins with a foundational overview of micropillar‐based cell mechanobiology studies to provide a complete understanding, and then it delves into novel methodologies within each domain. The latter part of the review unveils innovative micropillars’ applications beyond mechanobiology, such as their use in enhancing biosensing surfaces and as upstream fluid manipulators for biosensors. Finally, in this review, future research directions are discussed and the current limitations of these techniques are outlined. Despite the extensive exploration of micropillar applications, a significant gap remains between research advancements and the practical implementation of micropillars in point‐of‐care diagnostics. Bridging this gap is crucial for translating laboratory innovations into real‐world medical and diagnostic tools.

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Micropillars in Cell Mechanobiology: Design, Fabrication, Characterization, and Biosensing Applications

De Saram,

Nguyen,

Jamali

et al. 2024

Small Science

View full text Add to dashboard Cite

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CRISPR-based strategies for sample-to-answer monkeypox detection: current status and emerging opportunities

Ahamed,

Politza,

Liu

et al. 2024

Nanotechnology

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The global health threat posed by the Monkeypox virus (Mpox) requires swift, simple, and accurate detection methods for effective management, emphasizing the growing necessity for decentralized point-of-care (POC) diagnostic solutions. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), initially known for its effective nucleic acid detection abilities, presents itself as an attractive diagnostic strategy. CRISPR offers exceptional sensitivity, single-base specificity, and programmability. Here, we reviewed the latest developments in CRISPR-based POC devices and testing strategies for Mpox detection. We explored the crucial role of genetic sequencing in designing crRNA for CRISPR reaction and understanding Mpox transmission and mutations. Additionally, we showed the integration of CRISPR-Cas12 strategy with pre-amplification and amplification-free methods. Our study also focused on the significant role of Cas12 proteins and the effectiveness of Cas12 coupled with recombinase polymerase reaction (RPA) for Mpox detection. We envision the future prospects and challenges, positioning CRISPR-Cas12-based POC devices as a frontrunner in the next generation of molecular biosensing technologies.

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CRISPR-Based Biosensors for Medical Diagnosis: Readout from Detector-Dependence Detection Toward Naked Eye Detection

Hu,

Yin,

Bai

et al. 2024

Biosensors

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The detection of biomarkers (such as DNA, RNA, and protein) plays a vital role in medical diagnosis. The CRISPR-based biosensors utilize the CRISPR/Cas system for biometric recognition of targets and use biosensor strategy to read out biological signals without the employment of professional operations. Consequently, the CRISPR-based biosensors demonstrate great potential for the detection of biomarkers with high sensitivity and specificity. However, the signal readout still relies on specialized detectors, limiting its application in on-site detection for medical diagnosis. In this review, we summarize the principles and advances of the CRISPR-based biosensors with a focus on medical diagnosis. Then, we review the advantages and progress of CRISPR-based naked eye biosensors, which can realize diagnosis without additional detectors for signal readout. Finally, we discuss the challenges and further prospects for the development of CRISPR-based biosensors.

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Micropillar enhanced FRET-CRISPR biosensor for nucleic acid detection

Abstract: High-aspect ratio micropillar arrays are fabricated to enhance the detection performance of FRET-CRISPR assay.

Cited by 5 publications

References 48 publications

Micropillars in Cell Mechanobiology: Design, Fabrication, Characterization, and Biosensing Applications

Micropillars in Cell Mechanobiology: Design, Fabrication, Characterization, and Biosensing Applications

CRISPR-based strategies for sample-to-answer monkeypox detection: current status and emerging opportunities

CRISPR-Based Biosensors for Medical Diagnosis: Readout from Detector-Dependence Detection Toward Naked Eye Detection

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