Microplasma Jet Arrays as a Therapeutic Choice for Fungal Keratitis

Park, Hyun Jung; Kim, Soon Hee; Ju, Hyung Woo; Lee, Hyesook; Lee, Yoonjin; Park, Sehyun; Yang, H. J.; Park, Sung Jin; Eden, J. G.; Yang, Jong In; Park, Chan Hum

doi:10.1038/s41598-018-20854-8

Cited by 24 publications

(21 citation statements)

References 35 publications

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“…Ever since cold atmospheric pressure plasmas were first applied for biomedical purposes 1 there has been a tremendous amount of investigation to the interactions of these non-thermal discharges with various bio-surfaces [2][3][4][5] . In addition it initiated a regained interest in plasma liquid interactions 6 and brought forth the field of plasma agriculture 7 .…”

mentioning

confidence: 99%

Revealing Plasma-Surface Interaction at Atmospheric Pressure: Imaging of Electric Field and Temperature inside the Targeted Material

Slikboer

Acharya

Sobota

et al. 2020

Sci Rep

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The plasma-surface interaction is studied for a low temperature helium plasma jet generated at atmospheric pressure using Mueller polarimetry on an electro-optic target. The influence of the AC kHz operating frequency is examined by simultaneously obtaining images of the induced electric field and temperature of the target. The technique offers high sensitivity in the determination of the temperature variation on the level of single degrees. Simultaneously, the evolution of the electric field in the target caused by plasma-driven charge accumulation can be measured with the threshold of the order of 105 V/m. Even though a specific electro-optic crystal is used to obtain the results, they are generally applicable to dielectric targets under exposure of a plasma jet when they are of 0.5 mm thickness, have a dielectric constant greater than 4 and are at floating potential. Other techniques to examine the induced electric field in a target do not exist to the best of our knowledge, making this technique unique and necessary. The influence of the AC kHz operating frequency is important because many plasma jet designs used throughout the world operate at different frequency which changes the time between the ionization waves and hence the leftover species densities and stability of the plasma. Results for our jet show a linear operating regime between 20 and 50 kHz where the ionization waves are stable and the temperature increases linearly by 25 K. The charge deposition and induced electric fields do not increase significantly but the surface area does increase due to an extended surface propagation. Additionally, temperature mapping using a 100 μm GaAs probe of the plasma plume area has revealed a mild heat exchange causing a heating of several degrees of the helium core while the surrounding air slightly cools. This peculiarity is also observed without plasma in the gas plume.

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mentioning

confidence: 99%

Revealing Plasma-Surface Interaction at Atmospheric Pressure: Imaging of Electric Field and Temperature inside the Targeted Material

Slikboer

Acharya

Sobota

et al. 2020

Sci Rep

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“…Moreover, the expression of tumor necrosis factor-α and MMP-9 was recently shown to be significantly suppressed in plasma-treated fungal keratitis in rabbits, indicating that plasma exerts an antiinflammatory effect on the cornea. 32 …”

Section: Discussionmentioning

confidence: 99%

“…The LTP only affects the contact site with a depth of penetration of approximately 20 to 50 μm. 32 Hence, with appropriate energy, the affected depth and width can be controlled, making the LTP relatively safe for surrounding tissues such as the lens, iris, and retina. Our study used the VAS to quantify patient pain.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Safety and Efficacy of a Low-Temperature Plasma Surgical System for Pterygium

Huang

Zhong

et al. 2020

Cornea

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Purpose: Surgical excision is the standard treatment for pterygium. This study was conducted to evaluate the safety and efficacy of a novel technique using low-temperature plasma (LTP) for excision and hemostasis in pterygium surgery. Methods: A prospective, comparative, and randomized clinical trial was conducted on 60 patients (60 eyes) undergoing pterygium excision with conjunctival autografts using fibrin glue. Patients were equally divided into the following 2 groups: a control group and a LTP group. Postoperative follow-up visits were scheduled on day 1, week 1, and months 1 and 3, and recurrence was evaluated at 1 year. Patients were examined for operative time, best corrected visual acuity, conjunctival autograft inflammation (CAI), graft stability (GS), pain, recurrence, and final appearance. Factors related to pterygium recurrence and final appearance were analyzed. Results: Mean operative times were shorter in the LTP group (16.7 ± 3.4 min) than those in the control group (20.1 ± 4.7 min, P = 0.002). LTP eyes had milder CAI than control eyes at postoperative day 1 ( P = 0.000) and week 1 ( P = 0.000). Patients in the LTP group exhibited better GS ( P = 0.01) and milder pain ( P = 0.04) than those in the control group on day 1. Two control patients (6.7%) and no (0%) LTP patients experienced recurrence ( P = 0.08). GS and CAI were the significant factors contributing to recurrence (GS: R = 0.425, P = 0.001; CAI: R = 0.309, P = 0.016). Conclusions: LTP to replace surgical blades and disposable cautery for ablation and hemostasis is safe and efficient for pterygium surgery, resulting in shorter operative time, milder inflammation, and better graft stability without increasing complication risk.

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“…The former implies that blood-borne microbial infection has occurred as a result of metastatic spread of infection from other sites such as infected heart valves or the urinary tract. On the other hand, exogenous endophthalmitis results from a postoperative complication of lens removal, prosthetic lens implantation, or corneal transplantation [ 2 , 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Study the Antifungal and Ocular Permeation of Ketoconazole from Ophthalmic Formulations Containing Trans-Ethosomes Nanoparticles

et al. 2021

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Ketoconazole (KET), a synthetic imidazole broad-spectrum antifungal agent, is characterized by its poor aqueous solubility and high molecular weight, which might hamper its corneal permeation. The aim was to develop an ophthalmic formulation loaded with optimized trans-ethosomal vesicles to enhance KET ocular permeation, antifungal activity, rapid drug drainage, and short elimination half-life. Four formulation factors affecting the vesicles’ size, zeta potential, entrapment efficiency, and flexibility of the trans-ethosomes formulations were optimized. The optimum formulation was characterized, and their morphological and antifungal activity were studied. Different ophthalmic formulations loaded with the optimized vesicles were prepared and characterized. The ocular irritation and in vivo corneal permeation were investigated. Results revealed that the drug-to-phospholipid-molar ratio, the percentage of edge activator, the percentage of ethanol, and the percentage of stearyl amine significantly affect the characteristics of the vesicles. The optimized vesicles were spherical and showed an average size of 151.34 ± 8.73 nm, a zeta potential value of +34.82 ± 2.64 mV, an entrapment efficiency of 94.97 ± 5.41%, and flexibility of 95.44 ± 4.33%. The antifungal activity of KET was significantly improved following treatment with the optimized vesicles. The developed in situ gel formulations were found to be nonirritating to the cornea. The trans-ethosomes vesicles were able to penetrate deeper into the posterior eye segment without any toxic effects. Accordingly, the in situ developed gel formulation loaded with KET trans-ethosomes vesicles represents a promising ocular delivery system for the treatment of deep fungal eye infections.

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Microplasma Jet Arrays as a Therapeutic Choice for Fungal Keratitis

Cited by 24 publications

References 35 publications

Revealing Plasma-Surface Interaction at Atmospheric Pressure: Imaging of Electric Field and Temperature inside the Targeted Material

Revealing Plasma-Surface Interaction at Atmospheric Pressure: Imaging of Electric Field and Temperature inside the Targeted Material

Evaluation of the Safety and Efficacy of a Low-Temperature Plasma Surgical System for Pterygium

Study the Antifungal and Ocular Permeation of Ketoconazole from Ophthalmic Formulations Containing Trans-Ethosomes Nanoparticles

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