Microplastic accumulation patterns and transfer of benzo[a]pyrene to adult zebrafish (Danio rerio) gills and zebrafish embryos

Batel, Annika; Borchert, Flora; Reinwald, Hannes; Erdinger, Lothar; Braunbeck, Thomas

doi:10.1016/j.envpol.2018.01.028

Cited by 226 publications

(80 citation statements)

References 37 publications

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“…Bioaccumulation has been found in animal as well as in plant tissue with the consequence of ecotoxicity (Chua et al 2014;Chae and An 2017;Smith 2018). Toxicity can already occur by simple attachment of contaminated microplastics on epithelia of zebrafish, with serious effects of waterborne toxic substances on early life stages (Batel et al 2018). This shows that adherence rather than ingestion led to the accumulation of microplastics and associated toxicity (Batel et al 2018).…”

Section: Leaching Additives and Persistent Organic Pollutants: The Rementioning

confidence: 99%

“…Toxicity can already occur by simple attachment of contaminated microplastics on epithelia of zebrafish, with serious effects of waterborne toxic substances on early life stages (Batel et al 2018). This shows that adherence rather than ingestion led to the accumulation of microplastics and associated toxicity (Batel et al 2018). Furthermore, it is suggested that freshwater species suffer a higher risk, as the presence of salts in the water decrease the tendency of some chemicals to be sorbed onto plastic surfaces (Llorca et al 2018).…”

Section: Leaching Additives and Persistent Organic Pollutants: The Rementioning

confidence: 99%

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Understanding How Microplastics Affect Marine Biota on the Cellular Level Is Important for Assessing Ecosystem Function: A Review

Prinz

Korez

2019

YOUMARES 9 - The Oceans: Our Research, Our Future

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Plastic has become indispensable for human life. When plastic debris is discarded into waterways, these items can interact with organisms. Of particular concern are microscopic plastic particles (microplastics) which are subject to ingestion by several taxa. This review summarizes the results of cutting-edge research about the interactions between a range of aquatic species and microplastics, including effects on biota physiology and secondary ingestion. Uptake pathways via digestive or ventilatory systems are discussed, including (1) the physical penetration of microplastic particles into cellular structures, (2) leaching of chemical additives or adsorbed persistent organic pollutants (POPs), and (3) consequences of bacterial or viral microbiota contamination associated with microplastic ingestion. Following uptake, a number of individual-level effects have been observed, including reduction of feeding activities, reduced growth and reproduction through cellular modifications, and oxidative stress. Microplastic-associated effects on marine biota have become increasingly investigated with growing concerns regarding human health through trophic transfer. We argue that research on the cellular interactions with microplastics provide an understanding of their impact to the organisms' fitness and, therefore, its ability to sustain their functional role in the ecosystem. The review summarizes information from 236 scientific publications. Of those, only 4.6% extrapolate their research of microplastic intake on individual species to the impact on ecosystem functioning. We emphasize the need for risk evaluation from organismal effects to an ecosystem level to effectively evaluate the effect of microplastic pollution on marine environments. Further studies are encouraged to investigate sublethal effects in the context of environmentally relevant microplastic pollution conditions.

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Section: Leaching Additives and Persistent Organic Pollutants: The Rementioning

confidence: 99%

Section: Leaching Additives and Persistent Organic Pollutants: The Rementioning

confidence: 99%

Understanding How Microplastics Affect Marine Biota on the Cellular Level Is Important for Assessing Ecosystem Function: A Review

Prinz

Korez

2019

YOUMARES 9 - The Oceans: Our Research, Our Future

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“…BaP is ubiquitously distributed in coastal and offshore environments (Antunes et al, 2013;Liu et al, 2015;Châtel et al, 2017), and PAHs including BaP are known to adsorb at high rates to different types of MPs in seawater, particularly to PE (Rochman et al, 2013a;Ziccardi et al, 2016;Schönlau et al, 2019). Furthermore, BaP adsorbed to MPs has been documented to be transferred via trophic transfer to biota (Batel et al, 2016(Batel et al, , 2018Bour et al, 2018;O'Donovan et al, 2018;Pittura et al, 2018).…”

mentioning

confidence: 99%

Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio)

Cormier

Batel

Cachot

et al. 2019

Front. Environ. Sci.

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As wide-spread pollutants in the marine environment, microplastics (MPs) have raised public concern about potential toxic effects in aquatic organisms, and, among others, MPs were suspected to act as a vector for organic pollutants to biota. The purpose of the present study was to investigate effects by three model pollutants, oxybenzone (BP3), benzo[a]pyrene (BaP), and perfluorooctane sulfonate (PFOS) adsorbed to polyethylene MPs on the basis of a standard assay, the acute fish embryo toxicity test (FET; OECD TG 236) with zebrafish (Danio rerio) supplemented by additional endpoints such as induction of ethoxyresorufin-O-deethylase (EROD) activity, modification of cyp1a gene transcription and changes in larval swimming behavior. FET assays were performed in three laboratories using slightly different husbandry and exposure conditions, which, however, were all fully compatible with the limits defined by OECD TG 236. This allowed for testing of potential changes in the FET assay due to protocol variations. The standard endpoints of the FET (acute embryotoxicity) did not reveal any acute toxicity for both virgin MPs and MPs spiked with BP3, BaP, and PFOS. With respect to sublethal endpoints, EROD activity was increased after exposure to MPs spiked with BP3 (3 h pulse) and MPs spiked with BaP (96 h continuous exposure). Cyp1a transcription was increased upon exposure to MPs spiked with BP3 or BaP. For the selected combination of MPs particles and contaminants, the basic FET proved not sensitive enough to reveal effects of (virgin and spiked) MPs. However, given that the FET can easily be supplemented by a broad variety of more subtle and sensitive endpoints, an enhanced FET protocol may provide a relevant approach with developmental stages of a vertebrate animal model, which is not protected by current EU animal welfare legislation (Directive EU 2010/63).

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“…Similarly to our results, studies assessing the ecotoxicity of PE MPs on fish exposed via trophic chains also reported no effects [37][38][39] . Moreover, most studies exposing fish via water showed either no effects 34,[40][41][42][43] or mild effects, which include inhibition of AChE activity, impaired energy reserves, decreased swimming and predatory performances, changes in plasma biochemistry and histological changes in gills [44][45][46][47][48] . The adverse effects observed with pristine PE MPs in these studies could be due to the presence of monomers or additives 5 sorbed on MPs but that were not reported, either because no chemical analysis was performed or because concentrations were below limits of detection.…”

Section: Effect Of Contaminantsmentioning

confidence: 99%

Microplastic vector effects: are fish at risk when exposed via the trophic chain?

Bour

Sturve

Höjesjö

et al. 2019

Preprint

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In aquatic organisms, trophic transfer is an important exposure route for microplastics (MPs).Despite their relevance, effect studies on fish exposed via trophic chains are currently very scarce.MPs are known to contain many chemicals that could be transferred to organisms and induce deleterious effects. However, there is currently no consensus on whether MPs represent a significant exposure pathway to chemicals in contaminated habitats. Here, we exposed threespined stickleback (Gasterosteus aculeatus) to polyethylene MPs via prey ingestion, in a longterm experiment. MPs were either pristine or spiked with chlorpyrifos (CPF), and a CPF control was included to compare vector effects of MPs and natural prey. No effect was observed in fish exposed to pristine MPs. CPF accumulation was observed in fish exposed to CPF-spiked MPs (MP-CPF), confirming the vector potential of MPs. However, CPF accumulation was more important in fish exposed to CPF via prey. In fish exposed to MP-CPF, we observed significant AChE inhibition and hyperactivity, which could result in increased vulnerability to predation. CPF organ distribution differed between groups, suggesting that chemical exposure via MPs could alter organ distribution of chemicals. This can result in a change in the organs most at risk, likely increasing intestine exposure.

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Microplastic accumulation patterns and transfer of benzo[a]pyrene to adult zebrafish (Danio rerio) gills and zebrafish embryos

Cited by 226 publications

References 37 publications

Understanding How Microplastics Affect Marine Biota on the Cellular Level Is Important for Assessing Ecosystem Function: A Review

Understanding How Microplastics Affect Marine Biota on the Cellular Level Is Important for Assessing Ecosystem Function: A Review

Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio)

Microplastic vector effects: are fish at risk when exposed via the trophic chain?

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