Microplastic biofilm in fresh- and wastewater as a function of microparticle type and size class

Parrish, Kathleen; Fahrenfeld, Nicole

doi:10.1039/c8ew00712h

Cited by 115 publications

(54 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The strain was cultured anaerobically at 37 °C in MRS broth supplemented with 0.5 g/L L-cysteine hydrochloride-monohydrate. Overnight B. longum FGSZY16M3 culture was inoculated (4%, v / v ) into Erlenmeyer flask containing 60 mL MRS medium, and the culture was grown at 37 °C with 5% ( v / v ) oxygen [ 28 ] and 120 rpm for 86 h. The hollow glass microspheres (GM) with an average diameter of 50 μm and density of 0.48 g/cm 3 (Sinosteel maanshan general institute of mining research Co., LTD, Maanshan, China) were added to culture media with 1 g/L for biofilm culture [ 29 , 30 ]. No GM added groups were used as planktonic culture.…”

Section: Methodsmentioning

confidence: 99%

Transcriptome Analysis Reveals the Genes Involved in Bifidobacterium Longum FGSZY16M3 Biofilm Formation

Liu

Wang

et al. 2021

Microorganisms

View full text Add to dashboard Cite

Biofilm formation has evolved as an adaptive strategy for bacteria to cope with harsh environmental conditions. Currently, little is known about the molecular mechanisms of biofilm formation in bifidobacteria. A time series transcriptome sequencing analysis of both biofilm and planktonic cells of Bifidobacterium longum FGSZY16M3 was performed to identify candidate genes involved in biofilm formation. Protein–protein interaction network analysis of 1296 differentially expressed genes during biofilm formation yielded 15 clusters of highly interconnected nodes, indicating that genes related to the SOS response (dnaK, groS, guaB, ruvA, recA, radA, recN, recF, pstA, and sufD) associated with the early stage of biofilm formation. Genes involved in extracellular polymeric substances were upregulated (epsH, epsK, efp, frr, pheT, rfbA, rfbJ, rfbP, rpmF, secY and yidC) in the stage of biofilm maturation. To further investigate the genes related to biofilm formation, weighted gene co-expression network analysis (WGCNA) was performed with 2032 transcript genes, leading to the identification of nine WGCNA modules and 133 genes associated with response to stress, regulation of gene expression, quorum sensing, and two-component system. These results indicate that biofilm formation in B. longum is a multifactorial process, involving stress response, structural development, and regulatory processes.

show abstract

Section: Methodsmentioning

confidence: 99%

Transcriptome Analysis Reveals the Genes Involved in Bifidobacterium Longum FGSZY16M3 Biofilm Formation

Liu

Wang

et al. 2021

Microorganisms

View full text Add to dashboard Cite

show abstract

“…Polymer type is the most frequently investigated factor among all the microplastics characteristics because it directly affects microplastic biofilm formation. For example, in the Bay of Brest, microbial community composition on polyethylene and polypropylene was significantly distinct from that on polystyrene at a local scale (Frere et al 2018 ; Parrish and Fahrenfeld 2019 ) and in the ocean on a global scale (Amaral-Zettler et al 2015 ). Microbial biofilms on polyethylene terephthalate, polyethylene and polystyrene mesoplastics were dominated by Alphaproteobacteria and Gammaproteobacteria , while Burkholderiales (formerly Betaproteobacteria ) dominated on polyethylene microplastic biofilms in the North Atlantic garbage patch (Debroas et al 2017 ).…”

Section: Factors Influencing the Formation Of Microplastic Biofilmsmentioning

confidence: 99%

“…Consequently, microbial community structure and function on aged-microplastics requires detailed evaluation. Effects of microplastics size on microbial community composition, however, have not been observed (Frere et al 2018 ; Parrish and Fahrenfeld 2019 ), and there were no differences in bacterial community composition between microplastic and mesoplastic biofilms in the North Pacific Gyre (Bryant et al 2016 ). In addition to size, no obvious effects of plastic shape (monofilament, sheet, etc.)…”

Section: Factors Influencing the Formation Of Microplastic Biofilmsmentioning

confidence: 99%

Microplastics provide new microbial niches in aquatic environments

Yang

Liu

Zhang

et al. 2020

Appl Microbiol Biotechnol

305

View full text Add to dashboard Cite

Microplastics in the biosphere are currently of great environmental concern because of their potential toxicity for aquatic biota and human health and association with pathogenic microbiota. Microplastics can occur in high abundance in all aquatic environments, including oceans, rivers and lakes. Recent findings have highlighted the role of microplastics as important vectors for microorganisms, which can form fully developed biofilms on this artificial substrate. Microplastics therefore provide new microbial niches in the aquatic environment, and the developing biofilms may significantly differ in microbial composition compared to natural free-living or particle-associated microbial populations in the surrounding water. In this article, we discuss the composition and ecological function of the microbial communities found in microplastic biofilms. The potential factors that influence the richness and diversity of such microbial microplastic communities are also evaluated. Microbe-microbe and microbe-substrate interactions in microplastic biofilms have been little studied and are not well understood. Multiomics tools together with morphological, physiological and biochemical analyses should be combined to provide a more comprehensive overview on the ecological role of microplastic biofilms. These new microbial niches have so far unknown consequences for microbial ecology and environmental processes in aquatic ecosystems. More knowledge is required on the microbial community composition of microplastic biofilms and their ecological functions in order to better evaluate consequences for the environment and animal health, including humans, especially since the worldwide abundance of microplastics is predicted to dramatically increase. Key Points • Bacteria are mainly studied in community analyses: fungi are neglected. • Microbial colonization of microplastics depends on substrate, location and time. • Community ecology is a promising approach to investigate microbial colonization. • Biodegradable plastics, and ecological roles of microplastic biofilms, need analysis.

show abstract

“…There have been investigations into freshwater MP biofilms with a focus on using sequencing techniques to describe the microbial ecology (Eckert et al, 2018; Miao et al, 2019; Parrish & Fahrenfeld, 2019), biodegradation potential of MP or adsorbed organic compounds (Paço et al, 2017; Park & Kim, 2019; Porter, Wolfson, & Young, 2020), and the prevalence of antibiotic resistance genes and pathogens/pathogen marker genes (Parrish & Fahrenfeld, 2019; Rodrigues et al, 2019; Viršek, Lovšin, Koren, Kržan, & Peterlin, 2017). Fecal indicators such as E. coli have been cultivated from MP biofilms and pathogens such as Vibrio have been observed in MP biofilm found in the marine environment (Kirstein et al, 2016; Quilliam, Jamieson, & Oliver, 2014).…”

Section: Introductionmentioning

confidence: 99%

Total coliform and Escherichia coli in microplastic biofilms grown in wastewater and inactivation by peracetic acid

Boni

Parrish

Patil

et al. 2020

Water Environment Research

Self Cite

View full text Add to dashboard Cite

Microplastics (MP) have been proposed as a vector for pathogenic microorganisms in the freshwater environment. The objectives of this study were (a) to compare the fecal indicator growth in biofilms on MP and material control microparticles incubated in different wastewater fractions and (b) to compare MP biofilm, natural microparticle biofilm, and planktonic cell susceptibility to disinfection by peracetic acid (PAA). Biofilms were grown on high‐density polyethylene, low‐density polyethylene, polypropylene MP, or wood chips (as a material control) and incubated in either wastewater influent or pre‐disinfection secondary effluent. Reactors were disinfected with PAA, biofilms were dislodged, and total coliform and Escherichia coli were cultivated. Fecal indicators were quantifiable in both MP and wood biofilms incubated in the wastewater influent but only on the wood biofilms incubated in secondary wastewater effluent. More total coliform grew in the wood biofilms than MP biofilms, and the biofilms grown on MP and woodchips were more resistant to disinfection than planktonic bacteria. Thus, it may be possible to refer to the disinfection literature for fecal indicators in biofilm on other particles to predict behavior on MP. Treatments that remove particles in general would help reduce the potential for fecal indicator bypass of disinfection. Practitioner points MP biofilm had lower concentrations of fecal indicators than wood biofilm Biofilm on MP was not more resistant to disinfection than wood biofilm Biofilms, regardless of substrate, were more resistant to disinfection than planktonic organisms

show abstract

Microplastic biofilm in fresh- and wastewater as a function of microparticle type and size class

Abstract: Microplastics are pollutants of concern in the freshwater and marine environments. These microparticles carry biofilm communities unique from the surrounding water.

Cited by 115 publications

References 44 publications

Transcriptome Analysis Reveals the Genes Involved in Bifidobacterium Longum FGSZY16M3 Biofilm Formation

Transcriptome Analysis Reveals the Genes Involved in Bifidobacterium Longum FGSZY16M3 Biofilm Formation

Microplastics provide new microbial niches in aquatic environments

Total coliform and Escherichia coli in microplastic biofilms grown in wastewater and inactivation by peracetic acid

Contact Info

Product

Resources

About