Microplastic ingestion induces behavioral disorders in mice: A preliminary study on the trophic transfer effects via tadpoles and fish

Araújo, Amanda Pereira da Costa; Malafaia, Guilherme

doi:10.1016/j.jhazmat.2020.123263

Cited by 131 publications

(39 citation statements)

References 83 publications

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“…Microplastics have been found to be able to enter tissues of living organisms, including humans, via ingestion, inhalation, and topical absorption (Deng et al 2017 ; Huffer et al 2018 ; Pivokonsky et al 2018 ; Prata et al 2020 ; Zarus et al 2021 ; Zhang et al 2020b ). Although the full spectrum of their effects and mechanisms is yet to be determined, microplastic bioaccumulation in multiple biota through higher trophic levels has the potential to amplify their effects by their increasing dosage in top predators (such as humans) and can affect functions as varied as feeding behaviors, metabolism, and fecundity (da Costa Araujo and Malafaia 2021 ; Foley et al 2018 ). Sampling different environments and geographic locations has shown microplastic burdens of varying abundance, types, sizes, shapes, and ages, contributing to a variety of health dangers including bacterial antibiotic resistance (Su et al 2021 ; Wang et al 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Polystyrene and Polyethylene Microplastics Decrease Cell Viability and Dysregulate Inflammatory and Oxidative Stress Markers of MDCK and L929 Cells In Vitro

2021

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Microplastics are ubiquitous environmental pollutants that are a growing concern to many ecosystems, as well as human health. Many of the effects of microplastics on mammalian cells and tissues remain unknown. To address this, we treated L929 murine fibroblasts and Madin–Darby canine kidney (MDCK) epithelial cell lines with 1 μg/mL, 10 μg/mL, or 20 μg/mL of polyethylene (PE) or polystyrene (PS) microspheres in vitro for 6 and 24 h and measured the resulting changes in cell viability, metabolism, and transcriptional expression of inflammatory cytokines and antioxidant enzymes. We observed dose-dependent decreases in cell viability corresponding to increases in doses of both PE and PS. We conducted cell metabolism assays and observed dose-dependent increases in metabolism per cell with increasing doses of both PE and PS. Similarly, we also observed increased expression of the superoxide dismutase-3 gene ( SOD3 ), indicating oxidative stress caused by the microplastics treatments. We also observed increased expression of TNFα , but decreased expression of IFNβ , suggesting different mechanisms by which the microplastics regulate inflammatory responses in mammalian cells. Our results contribute new data to the growing understanding of the effects of microplastics on mammalian cells and indicate complex cellular stress responses to microplastics in the environment.

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Section: Introductionmentioning

confidence: 99%

Polystyrene and Polyethylene Microplastics Decrease Cell Viability and Dysregulate Inflammatory and Oxidative Stress Markers of MDCK and L929 Cells In Vitro

2021

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“…Recently, several animal studies have been published on the effects of MPs (26)(27)(28)(29). MP ingestion may induce behavioral disorders in mice (30). Therefore, it is important to observe how MPs are distributed in the body following ingestion.…”

Section: Dota-nhs Ester and P-scn-bn-dota Are Frequently Used Chelators (19) Dota Conjugationmentioning

confidence: 99%

PET Tracing of Biodistribution for Orally Administered ⁶⁴Cu-Labeled Polystyrene in Mice

Kim

Zaheer

et al. 2021

J Nucl Med

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Purpose: Plastics are used commonly in the world because of its convenience and costeffectiveness. Microplastics, an environmental threat and human health risk, are widely detected in food, and consequently ingested. However degraded plastics are found everywhere, which cause environmental threat and human health risk. Therefore, real-time monitoring of orally administered microplastics is tremendously important to trace them in the body.Methods: In this study, to visualize their absorption path, we labeled polystyrene with [ 64 Cu]Cu-DOTA. We prepared radiolabeled polystyrene with 64 Cu after, [ 64 Cu]Cu-DOTApolystyrene was then orally administered to mice and evaluate its transit and absorption in mice using PET imaging. The absorption path and distribution of [ 64 Cu]Cu-DOTA-polystyrene were determined using positron emission tomography (PET) over 48 h. Ex vivo tissue-radio thin-layer chromatography (Ex vivo-radioTLC) was used to demonstrate the existence of [ 64 Cu]Cu-DOTApolystyrene in tissue. Results: PET images demonstrated that [ 64 Cu]Cu-DOTA-polystyrene began to transit to the intestine within 1 h. [ 64 Cu]Cu-DOTA-polystyrene accumulation in the liver was also observed. Biodistribution of [ 64 Cu]Cu-DOTA-polystyrene confirmed the observed distribution of [ 64 Cu]Cu-DOTA-polystyrene from PET images. Ex vivo-radioTLC was used to demonstrate that the detected gamma rays originated from [ 64 Cu]Cu-DOTA-polystyrene. Conclusion:This study provided evidence of microplastic accumulation and existence in tissue by using PET imaging, and cross confirmed by ex vivo-radioTLC. The information provided may be used as the basis for future studies on the toxicity of microplastics.

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“…In a pioneering study, trophic transfer of PE MP (~35.5 μm) through the food chain was demonstrated in Swiss mice fed with tambatinga fish along with associated behavioral changes predicting neurotoxicity (17). The tambatinga fish were earlier fed with tadpoles (Physalaemus cuvieri) that were exposed to MP (60 mg/L or 4.24 3 10 À6 particles per m 3 in aquarium water) for 7 days.…”

Section: Toxicological Consequences Of Mp and Np Exposure In Rodentsmentioning

confidence: 99%

Micro- and Nanoplastic-Mediated Pathophysiological Changes in Rodents, Rabbits, and Chickens: A Review

Banerjee

Shelver

2021

Journal of Food Protection

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Plastics provide tremendous societal benefits and are an indispensable part of our lives. However, fragmented plastics or those intentionally manufactured in small sizes (microplastics or nanoplastics) are of concern because they can infiltrate soils and enter the human food chain through trophic transfer. The pathophysiological impacts of micro/nanoplastics in humans are not characterized but their effects in terrestrial mammals may help elucidate their potential effects in human beings. Rodent studies have demonstrated that micro/nanoplastics can breach the intestinal barrier, accumulate in various organs, cause gut dysbosis, decrease mucus secretion, induce metabolic alterations, and cause neurotoxicity, amongst other pathophysiologic effects. Larger mammals such as rabbits can also absorb microplastics orally. In farm animals such as chicken, microplastics have been detected in the gut, thereby raising food safety concerns. This review mostly focuses on studies conducted to assess effects of micro/nanoplastic exposure through food and water in terrestrial mammals and farm animals including rodents, rabbit and chicken, identifies main knowledge gaps, and provides recommendations for further research to understand food-borne MP/NP toxicity in humans.

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Microplastic ingestion induces behavioral disorders in mice: A preliminary study on the trophic transfer effects via tadpoles and fish

Cited by 131 publications

References 83 publications

Polystyrene and Polyethylene Microplastics Decrease Cell Viability and Dysregulate Inflammatory and Oxidative Stress Markers of MDCK and L929 Cells In Vitro

Polystyrene and Polyethylene Microplastics Decrease Cell Viability and Dysregulate Inflammatory and Oxidative Stress Markers of MDCK and L929 Cells In Vitro

PET Tracing of Biodistribution for Orally Administered ⁶⁴Cu-Labeled Polystyrene in Mice

Micro- and Nanoplastic-Mediated Pathophysiological Changes in Rodents, Rabbits, and Chickens: A Review

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Microplastic ingestion induces behavioral disorders in mice: A preliminary study on the trophic transfer effects via tadpoles and fish

Cited by 131 publications

References 83 publications

Polystyrene and Polyethylene Microplastics Decrease Cell Viability and Dysregulate Inflammatory and Oxidative Stress Markers of MDCK and L929 Cells In Vitro

Polystyrene and Polyethylene Microplastics Decrease Cell Viability and Dysregulate Inflammatory and Oxidative Stress Markers of MDCK and L929 Cells In Vitro

PET Tracing of Biodistribution for Orally Administered 64Cu-Labeled Polystyrene in Mice

Micro- and Nanoplastic-Mediated Pathophysiological Changes in Rodents, Rabbits, and Chickens: A Review

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PET Tracing of Biodistribution for Orally Administered ⁶⁴Cu-Labeled Polystyrene in Mice