This paper provides a detailed description of the findings and methodology related to the monitoring of microplastics in three lakes and one river of the Akmola Region in Kazakhstan. The concentration of microplastic particles and the analysis of water and sediment quality of the Yesil River and Kopa, Zerendinskoye, and Borovoe lakes have been analyzed. A total of 64 water samples were collected across the spring, summer, and autumn seasons, with subsequent analysis revealing a seasonal increase in microplastic concentrations. The average microplastic content ranged from 1.2 × 10−1 particles/dm3 in spring to 4.5 × 10−1 particles/dm3 in autumn. Lakes exhibited higher concentrations compared to the Yesil River. Correlation analysis highlighted a connection between microplastic content and turbidity, particularly notable during the spring season. Analysis of sediments revealed a decrease in microplastic concentrations from the coastal zone toward open waters sediments. Microplastic fibers were predominant in sediments (69.6%), followed by fragments (19.1%), films (7.4%), and granules (3.9%). Larger particles (>500 µm) were found in beach sediments, constituting an average of 40.5% of the total plastics found. This study contributes valuable insights into the spatial and temporal distribution of microplastics, emphasizing the need for ongoing monitoring and management strategies to address this environmental concern.