Microplastics: Detection in human samples, cell line studies, and health impacts

Barceló, Damià; Picó, Yolanda; Alfarhan, Ahmed

doi:10.1016/j.etap.2023.104204

Cited by 76 publications

(10 citation statements)

References 50 publications

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“…Microplastics are then eliminated into the bile or the gastrointestinal lumen, where they will be excreted in the feces [127] . Concentrations as high as 139 microplastics•g -1 have been found in human feces [128] Additionally, microplastics have been found in human samples including saliva, sputum, bronchoalveolar lavage fluid, pulmonary tissue, blood, liver, kidney, placenta, and breast milk [129] .…”

Section: Effects Of Microplastics On Human Healthmentioning

confidence: 99%

Do’s and don’ts of microplastic research: a comprehensive guide

Prata,

Padrão,

Khan

et al. 2024

Water Emerg Contam Nanoplastics

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Microplastics are environmental contaminants consisting of small plastics ≤ 5 mm. Concerns over the adverse effects of microplastics have led to a rapid growth in the available literature despite the lack of harmonized methods and materials. Therefore, the field is becoming increasingly daunting to new researchers. A state-of-the-art guide was assembled following a comprehensive literature review of microplastics research with the intent of addressing contemporary challenges, prioritized based on a survey, and introducing best practices. The lack of standardized methods and reference materials, the lack of access to analytical equipment, and the difficulty in working with lower environmental concentrations in laboratory tests (e.g., toxicity assays) remain a great challenge. The present work addresses these issues across three main sections: definitions, sampling, and evaluation of adverse effects. Harmonized methods and greater collaboration were identified as opportunities in this rapidly evolving field. A review of available interlaboratory comparison tests was also conducted to support additional recommendations.

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Section: Effects Of Microplastics On Human Healthmentioning

confidence: 99%

Do’s and don’ts of microplastic research: a comprehensive guide

Prata,

Padrão,

Khan

et al. 2024

Water Emerg Contam Nanoplastics

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“…9–11 There are reports on human cell line studies proving microplastics to be toxic to humans. 12–14 All these factors urge us to decipher the mechanism of microplastic toxicity at the molecular level, which will help us gauge the threat and take precautionary measures.…”

Section: Introductionmentioning

confidence: 99%

The impact of nano-polystyrene on human serum albumin–paracetamol interactions: understanding the impact on therapeutic development and safety

Sunil,

Thomas,

Shivashankar

et al. 2024

Environ. Sci.: Nano

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“…Once plastic enters the environment, it starts breaking down into tiny particles referred to as microplastics (MPs; 1 to 1000 μm) and nanoplastics (NPs; 1 to 100 nm, occasionally up to 500 nm). These plastic particles can then make their way into the food chain through ingestion (Ng et al., 2018) and their subsequent detection in human placental tissues (Amereh et al., 2022; Barcelo et al., 2023) and blood (Banerjee & Shelver, 2021; Leslie et al., 2022) has been widely reported. The intestinal epithelium in the mammalian gut serves as a protective barrier and as the primary site of food uptake (Kraehenbuhl et al., 1997).…”

Section: Introductionmentioning

confidence: 99%

Impact of Micro‐ and Nano‐Plastics on Human Intestinal Organoid‐Derived Epithelium

Wang,

Iglesias‐Ledon,

Bishop

et al. 2024

Current Protocols

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The development of patient‐derived intestinal organoids represents an invaluable model for simulating the native human intestinal epithelium. These stem cell‐rich cultures outperform commonly used cell lines like Caco‐2 and HT29‐MTX in reflecting the cellular diversity of the native intestinal epithelium after differentiation. In our recent study examining the effects of polystyrene (PS), microplastics (MPs), and nanoplastics (NPs), widespread pollutants in our environment and food chain, on the human intestinal epithelium, these organoids have been instrumental in elucidating the absorption mechanisms and potential biological impacts of plastic particles. Building on previously established protocols in human intestinal organoid culture, we herein detail a streamlined protocol for the cultivation, differentiation, and generation of organoid‐derived monolayers. This protocol is tailored to generate monolayers incorporating microfold cells (M cells), key for intestinal particle uptake but often absent in current in vitro models. We provide validated protocols for the characterization of MPs/NPs via scanning electron microscopy (SEM) for detailed imaging and their introduction to intestinal epithelial monolayer cells via confocal immunostaining. Additionally, protocols to test the impacts of MP/NP exposure on the functions of the intestinal barrier using transendothelial electrical resistance (TEER) measurements and assessing inflammatory responses using cytokine profiling are detailed. Overall, our protocols enable the generation of human intestinal organoid monolayers, complete with the option of including or excluding M cells, offering crucial techniques for observing particle uptake and identifying inflammatory responses in intestinal epithelial cells to advance our knowledge of the potential effects of plastic pollution on human gut health. These approaches are also amendable to the study of other gut‐related chemical and biological exposures and physiological responses due to the robust nature of the systems. © 2024 Wiley Periodicals LLC.Basic Protocol 1: Human intestinal organoid culture and generation of monolayers with and without M cellsSupport Protocol 1: Culture of L‐WRN and production of WRN‐conditioned mediumSupport Protocol 2: Neuronal cell culture and integration into intestinal epitheliumSupport Protocol 3: Immune cell culture and integration into intestinal epitheliumBasic Protocol 2: Scanning electron microscopy: sample preparation and imagingBasic Protocol 3: Immunostaining and confocal imaging of MP/NP uptake in organoid‐derived monolayersBasic Protocol 4: Assessment of intestinal barrier function via TEER measurementsBasic Protocol 5: Cytokine profiling using ELISA post‐MP/NP exposure.

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Microplastics: Detection in human samples, cell line studies, and health impacts

Cited by 76 publications

References 50 publications

Do’s and don’ts of microplastic research: a comprehensive guide

Do’s and don’ts of microplastic research: a comprehensive guide

The impact of nano-polystyrene on human serum albumin–paracetamol interactions: understanding the impact on therapeutic development and safety

Impact of Micro‐ and Nano‐Plastics on Human Intestinal Organoid‐Derived Epithelium

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