Microporous poly(caprolactam) hollow fibers for therapeutic affinity adsorption

Kugel, Kerstin; Moseley, Amy E.; Harding, George B.; Klein, Elias

doi:10.1016/0376-7388(92)87077-b

Cited by 53 publications

(20 citation statements)

References 14 publications

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“…Beside ongoing research in protein chromatographic systems, hydrophilic microfiltration membranes with functional sites that can be used for binding (bio)affinity ligands are increasingly investigated as alternative adsorbents [1,2].…”

Section: Introductionmentioning

confidence: 99%

Adsorptive membranes for bilirubin removal

Avramescu

Sager

Borneman

et al. 2004

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Adsorptive membranes for bilirubin removal

Avramescu

Sager

Borneman

et al. 2004

Journal of Chromatography B

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“…The ninhydrin method [32] was employed to determine amount of the amine end-groups in resulting membranes, which was about one-fourth of total nitrogen atom in PEI without crosslinking and can show the content of PEI in membranes ignoring the crosslinked proportion of PEI.…”

Section: Amine End-groups Measurementmentioning

confidence: 99%

Preparation and performance of cellulose acetate/polyethyleneimine blend microfiltration membranes and their applications

Chen

Deng

Chen

et al. 2004

Journal of Membrane Science

174

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“…Flow rate: 6.0 mL min-1; other experimental conditions as Figure 6. immuno-complex in the blood and may be treated by immuno-adsorption therapy [25][26][27]. The immunoaffinity analysis of dog IgG in plasma in a clinical experiment in immuno-adsorption therapy was performed on the protein A column and the chromatograms obtained are shown in Figure 6.…”

Section: Resultsmentioning

confidence: 99%

Membrane affinity chromatography for analysis and purification of biopolymers

Zhou

Zou

et al. 1999

Chromatographia

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KeyWordsColumn liquid chromatography Membrane stationary phase Immunoaffinity analysis Affinity efficiency Adsorption capacity SummaryAffinity columns suitable for HPLC were prepared by immobilization of various ligands of protein A, human IgG, human IgM and pectinase on GMA modified cellulose membrane. The adsorption capacity, affinity efficiency and activity recovery of various IgGs on these affinity columns were measured. It was observed that the length of the coupling arm plays a very important role in affinity efficiency, and the effect of eluent flow-rate on adsorption capacity was very small. The protein A column was exploited for the process monitoring of dog IgG in clinical experiments on immuno-adsorption therapy. A pectinase column was used for the determination of polygalacturonase inhibiting proteins first purified on a hydroxyapatite column. It took only about 2.5 min for analysis at a flow-rate of 1.0 mL min -1. The high speed analysis of biopolymers could be performed at a flow rate of 6.0 1 mL rain-within 15 s. Membrane affinity chromatography gives good reproducibility, high efficiency, low column-pressure and is rapid. It can also be used for micro-scale purification ofbiopolymers.

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Microporous poly(caprolactam) hollow fibers for therapeutic affinity adsorption

Cited by 53 publications

References 14 publications

Adsorptive membranes for bilirubin removal

Adsorptive membranes for bilirubin removal

Preparation and performance of cellulose acetate/polyethyleneimine blend microfiltration membranes and their applications

Membrane affinity chromatography for analysis and purification of biopolymers

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