Microporous Poly(L-Lactic Acid) Membranes Fabricated by Polyethylene Glycol Solvent-Cast/Particulate Leaching Technique

Selvam, Shivaram; Chang, W. V.; Nakamura, T.; Samant, D. M.; Thomas, Padmaja B.; Trousdale, Melvin D.; Mircheff, Austin K.; Schechter, Joel; Yiu, Samuel C.

doi:10.1089/ten.tec.2008.0431

Cited by 20 publications

(29 citation statements)

References 46 publications

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“…However, because it is derived from mouse sarcoma tissue, there are obvious safety concerns associated with the use of Matrigel in a clinical setting (Kibbey, 1994). Several polymer-based substrates have also been tested or their acting as a scaffold in lacrimal gland morphogenesis with varying degrees of success being achieved (Long et al, 2006; Selvam et al, 2007a; Selvam et al, 2007b; Selvam et al, 2009). Long-term functional studies will be needed to evaluate the efficacy and stability of the engineered tissues.…”

Section: The Molecular Mechanism Of Lacrimal Gland Developmentmentioning

confidence: 99%

Lacrimal gland development: From signaling interactions to regenerative medicine

Garg

Zhang

2017

Developmental Dynamics

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The lacrimal gland plays a pivotal role in keeping the ocular surface lubricated, and protecting it from environmental exposure and insult. Dysfunction of the lacrimal gland results in deficiency of the aqueous component of the tear film, which can cause dryness of the ocular surface, also known as the aqueous-deficient dry eye disease. Left untreated, this disease can lead to significant morbidity, including frequent eye infections, corneal ulcerations and vision loss. Current therapies do not treat the underlying deficiency of the lacrimal gland, but merely provide symptomatic relief. To develop more sustainable and physiological therapies, such as in vivo lacrimal gland regeneration or bioengineered lacrimal gland implants, a thorough understanding of lacrimal gland development at the molecular level is of paramount importance. Based on the structural and functional similarities between rodent and human eye development, extensive studies have been undertaken to investigate the signaling and transcriptional mechanisms of lacrimal gland development using mouse as a model system. In this review, we describe the current understanding of the extrinsic signaling interactions and the intrinsic transcriptional network governing lacrimal gland morphogenesis, as well as recent advances in the field of regenerative medicine aimed at treating dry eye disease.

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Section: The Molecular Mechanism Of Lacrimal Gland Developmentmentioning

confidence: 99%

Lacrimal gland development: From signaling interactions to regenerative medicine

Garg

Zhang

2017

Developmental Dynamics

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“…The final characteristics (porosity and pore size) are strictly related to the adopted method selected for scaffold realization. A number of methods have been developed to produce porous 3D architectures, including solvent casting/particulate leaching and phase‐separation methods …”

Section: Introductionmentioning

confidence: 99%

Effect of processing techniques on the 3D microstructure of poly (l‐lactic acid) scaffolds reinforced with wool keratin from different sources

Puglia

Ceccolini

Fortunati

et al. 2015

J of Applied Polymer Sci

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The aim of this work was the analysis of morphologies of poly (l‐lactic acid)/keratin tridimensional scaffolds developed by using two main solvent based techniques: solvent casting particulate leaching in the presence of paraffin microspheres as porogen, and thermally induced phase‐separation process. Keratins from different sources were used. Specifically, keratin from Merino wool (KM) and Brown Alpaca (KA) were dispersed in dioxane or 1,4‐dioxane/water mixture and then the keratin structure after interaction with 1.4‐dioxane was investigated by means of electrophoresis and spectroscopic analysis. A detailed morphological characterization of the scaffold systems obtained by the two different methods was performed by using field emission scanning electron microscopy. The effect of porogen content, solvent composition and processing parameters, such as the influence of keratin presence (1 wt %) and source (KA and KM) were evaluated. The results reveal that 1,4‐dioxane, used as based solvent in the two selected processes, does not negatively affect the keratin structure. A slight change in the procedure parameters, such as porogen content or solvent/non‐solvent ratio, significantly affects the resulting porosity and architecture of the obtained PLLA and PLLA/keratin scaffolds. Both keratins can be considered as suitable fillers able to modify the pore architecture and interconnection of three‐dimensional PLLA/keratin porous composites obtained by the two different solvent assisted techniques. Of note, both composites are biocompatible, as demonstrated by the culture adult bone‐marrow mesenchymal stem cells without signs of toxicity. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 42890.

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“…Numerous studies describe the use of a variety of materials for scaffolds including polymers, metals, and ceramics. [2][3][4] To improve existing approaches, design cues can be taken from the bone tissue itself in a biomimetic process where the engineer attempts to recapitulate the building of the natural tissue in vitro. Bone is essentially a two phase nanocomposite tissue in which the organic matrix is the source of bone strength and toughness and the inorganic mineral the source of stiffness.…”

Section: Introductionmentioning

confidence: 99%

Remineralized bone matrix as a scaffold for bone tissue engineering

Soicher

Christiansen

Stover

et al. 2014

J. Biomed. Mater. Res.

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There is a need for improved biomaterials for use in treating non-healing bone defects. A number of natural and synthetic biomaterials have been used for the regeneration of bone tissue with mixed results. One approach is to modify native tissue via decellularization or other treatment for use as natural scaffolding for tissue repair. In this study, our goal was to improve on our previously published alternating solution immersion (ASI) method to fabricate a robust, biocompatible, and mechanically competent biomaterial from natural demineralized bone matrix (DBM). The improved method includes an antigen removal (AR) treatment step which improves mineralization and stiffness while removing unwanted proteins. The chemistry of the mineral in the remineralized bone matrix (RBM) was consistent with dicalcium phosphate dihydrate (brushite), a material used clinically in bone healing applications. Mass spectrometry identified proteins removed from the matrix with AR treatment to include α-2 HS-glycoprotein and osteopontin, non-collagenous proteins (NCPs) and known inhibitors of biomineralization. Additionally, the RBM supported the survival, proliferation, and differentiation of human mesenchymal stromal cells (MSCs) in vitro as well or better than other widely used biomaterials including DBM and PLG scaffolds. DNA content increased more than 10-fold on RBM compared to DBM and PLG; likewise, osteogenic gene expression was significantly increased after 1 and 2 weeks. We demonstrated that ASI remineralization has the capacity to fabricate mechanically stiff and biocompatible RBM, a suitable biomaterial for cell culture applications.

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Microporous Poly(L-Lactic Acid) Membranes Fabricated by Polyethylene Glycol Solvent-Cast/Particulate Leaching Technique

Cited by 20 publications

References 46 publications

Lacrimal gland development: From signaling interactions to regenerative medicine

Lacrimal gland development: From signaling interactions to regenerative medicine

Effect of processing techniques on the 3D microstructure of poly (l‐lactic acid) scaffolds reinforced with wool keratin from different sources

Remineralized bone matrix as a scaffold for bone tissue engineering

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