Microprojectile-Dna Delivery in Somatic Embryos of Olive (Olea Europaea L.)

Lambardi, Maurizio; Benelli̇, C.; Amorosi, S.; Branca, C.; Caricato, G.; Rugini, Eddo

doi:10.17660/actahortic.1999.474.104

Cited by 19 publications

(11 citation statements)

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“…Sunflower ubiquitin promoter yielded a significantly higher number of gus expressing foci per bombardment than CaMV35S promoter, even when the last one contains an enhancer which generally increases the strength of this constitutive promoter. Similarly, Lambardi et al (1999) found that the sunflower ubiquitin promoter yielded a higher number of gus expression units than the CaMV35S when olive embryos cv. 'Canino' of small size (at the torpedo stage) were bombarded by using the Particle Inflow Gun device.…”

Section: Discussionmentioning

confidence: 89%

“…bombardment pressure, target distance, microprojectile nature and size, that can vary substantially depending on the explant used (Sanford et al 1993). In olive, Lambardi et al (1999) In this investigation, we have evaluated several aspects of the genetic transformation of olive. The main goals of this study were: First, to determine the efficiency of different aminoglycoside antibiotics as selective agents.…”

Section: Introductionmentioning

confidence: 99%

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Studies on genetic transformation of olive (Olea europaea L.) somatic embryos: I. Evaluation of different aminoglycoside antibiotics for nptII selection; II. Transient transformation via particle bombardment

Pérez-Barranco

Torreblanca

Padilla

et al. 2009

Plant Cell Tiss Organ Cult

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As a first step to the establishment of a genetic transformation protocol for olive somatic embryos obtained from the seeds of cv. 'Picual', the efficiencies of different aminoglycoside antibiotics as selective agents to be used with the nptII marker gene, and the particle bombardment technique for transient transformation have been evaluated. Among the three antibiotics tested, paromomycin and kanamycin showed a similar inhibitory effect and, at 200 mg l -1 , both of them impaired callus growth after 8 weeks of culture. However, when isolated embryos were cultured in the presence of these antibiotics, a 20% of the embryos still remained viable at 400 mg l -1 . Neomycin was discarded as a selective agent since it showed only a moderate toxic effect. Contrary to solid medium, when olive callus was cultured in liquid medium supplemented with different paromomycin concentrations for 3 weeks, the callus growth was impaired at the lowest antibiotic concentration, 3 mg l -1 . Best conditions for transient transformation of olive callus using PDS-1000/He system were a 6 cm target distance and a 900 psi bombardment pressure. pCGUD1 plasmid, containing the gus gene under the control of sunflower ubiquitin promoter yielded a significantly higher number of gus expression areas per bombarded explant than pGUSINT or pJGUS5 plasmids, where the gus gene is driven by CaMV35S promoter or CaMV35S with enhancer, respectively. Almost 45% of bombarded explants showed gus expression 12 weeks after bombardment.

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Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Studies on genetic transformation of olive (Olea europaea L.) somatic embryos: I. Evaluation of different aminoglycoside antibiotics for nptII selection; II. Transient transformation via particle bombardment

Pérez-Barranco

Torreblanca

Padilla

et al. 2009

Plant Cell Tiss Organ Cult

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“…Most recently, transient expression in various tissues of woody species have been achieved by using microprojectile-mediated DNA delivery (Ellis et al, 1991;Goldfarb et al, 1991;Charest et al, 1993;Duchesne et al, 1993;Hay et al, 1994); however, genetic transformation requires the optimization of several factors, including the in vitro regeneration systems, the methodologies for DNA delivery, and a high expression of the introduced gene in the plant tissue. The only DNA delivery system being used in olive up till now is DNA delivery by microprojectile bombardment (Lambardi et al, 1999).…”

Section: Development Of Transgenic Olivesmentioning

confidence: 99%

“…Numerous somatic embryos easily develop with the ability to produce cyclic somatic embryogenesis from the epidermis or from subepidermal layer. The cyclic somatic embryogenesis (represented by normal and abnormal embryos) can be maintained indefinitely even in hormone-free medium, and some can also germinate (Figure 3) Lambardi et al, 1999;Benelli et al, 2000;Rugini et al, 2005). The conversion of normal embryos into plants is difficult; however, they in the field show juvenility with a retardation of blossom 3-4 years later than micropropagated plants by axillary bud stimulation.…”

Section: Somatic Embryogenesismentioning

confidence: 99%

“…For other species, the use of the rol genes for plant improvement have demonstrated production of drastic effects on the plant architecture (Rugini et al, 1991;van der Salm et al, 1997van der Salm et al, , 1998Perez-Molphe-Balch and Ochoa-Alejo 1998;Mercuri et al, 2001;Zhu et al, 2001), but scarce is the knowledge on the interaction of these genes with the genome of the Lambardi et al, 1999 plant. Moreover, the development of plants with an extensive root system and/or with reduced water consumption could be an advantage in areas with scarce water availability.…”

Section: Modifications Of Vegetative Habitsmentioning

confidence: 99%

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Olive

Rugini

Gutiérrez-Pesce

Muleo

2008

Compendium of Transgenic Crop Plants

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Biochemical and molecular procedures have been developed for olive genotyping, for taxonomy between cultivated and wild olives, for phylogenetic studies of cultivars, and for identification of usable markers linked to the most important agronomic traits, such as size of the tree, flowering induction, apical dominance, productivity, self‐fertility, quantity and quality of oil and secondary products of fruit, biotic and abiotic resistance. These characters could be introgressed into cultivars by conventional breeding or through transgeny. Successful results by classical breeding are difficult to obtain due to the different flower fertility and the long juvenile period of the progenies. The low efficiency of modern genetic improvement techniques suggests that genetic transformation techniques could speed up the development of new genotypes; however, the development of efficient regeneration methods from mature tissue is the limiting factor for applying this technology, in olive. Transgenic olive plants with modified growth habit and putative induced disease resistance are under filed conditions to test their phenotype. Other attempts of genetic improvement such as clonal selection, mutation through γ‐irradiation, protoplast technology, haploid, changes in ploidy level, somaclonal variation, and somatic hybridization are discussed and reviewed.

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