Micropropagation and assessment of genetic fidelity of Dendrocalamus strictus (Roxb.) nees using RAPD and ISSR markers

Abstract

Dendrocalamus strictus popularly known as ‘Male bamboo’ is a multipurpose bamboo which is extensively utilized in pharmaceutical, paper, agricultural and other industrial implements. In this study, in vitro regeneration of D. strictus through nodal culture has been attempted. Murashige and Skoog’s medium supplemented with 4 mg/l BAP was found to be most effective in shoot regeneration with 3.68 ± 0.37 shoots per explant. The effect of Kn was found to be moderate. These hormones also had considerable effect on the shoot length. The highest shoot length after 6 weeks (3.11 ± 0.41 cm) was noted with 5 mg/l BAP followed by 3.07 ± 0.28 cm with 5 mg/l Kn, while decrease in the shoot length was noted with other treatments. The effect of IBA and NAA individually or in combination at different concentrations on rooting was evaluated. The highest number of root (1.36 ± 0.04) was regenerated on full-strength MS medium supplemented with 3 mg/l NAA, while maximum length of 1.64 ± 0.03 cm of roots was recorded with combination of 1 mg/l IBA and 3 mg/l NAA. Tissue-cultured plants thus obtained were successfully transferred to the soil. The clonal fidelity among the in vitro-regenerated plantlets was assessed by RAPD and ISSR markers. The ten RAPD decamers produced 58 amplicons, while nine ISSR primers generated a total of 66 bands. All the bands generated were monomorphic. These results confirmed the clonal fidelity of the tissue culture-raised D. strictus plantlets and corroborated the fact that nodal culture is perhaps the safest mode for multiplication of true to type plants.

Plant regeneration from direct and indirect organogenesis and assessment of genetic fidelity in Saccharum officinarum using DNA-based markers

“…2. Similar results were reported in micro-propagated shoots of Curcuma longa (Salvi et al 2001), sugarcane (Khan et al 2009), Gerbera jasmesonii (Bhatia et al 2011), Momordica dioica (Rai et al 2012), Terminallia bellerica (Dangi et al 2014), and Dendrocalamus strictus (Goyal et al 2015). Zucchi et al (Zucchi et al 2002) reported that polymorphism occurred in sugarcane shoots regenerated from meristem cultures because of the number of subcultures and RAPD analysis was used for detection of polymorphism.…”

Plant regeneration from cell suspension culture in Saccharum officinarum L. and ascertaining of genetic fidelity through RAPD and ISSR markers

“…RAPD and ISSR has been successfully applied to determine genetic fidelity/stability in several micropropagated plant systems viz. sugarcane (Suprasanna et al 2006; Devarumath et al 2007; Lal et al 2008), Chlorophytum borivilianum (Rizvi et al 2012), Terminalia beelerica (Dangi et al 2014), Aloe barbandesis (Sahoo and Rout 2014), Swertia lawii (Kshirsagar et al 2015), Dendrocalamus strictus (Goyal et al 2015), Dendrocalamus hamiltonii (Singh et al 2013) etc. These markers have been successfully applied for the detection of polymorphisms that are either induced or incorporated during in vitro regeneration of several plant species (Asthana et al 2011).…”

Somatic Embryogenesis and Genetic Fidelity Study of the Micropropagated Medicinal Species, Canna indica

“…Callus induction was initiated in media containing different concentrations of 6-benzyl amino purine (BAP) as cytokinin and 1-napthaleneacetic acid (NAA) or 2,4 dichloro-phenoxy acetic acid (2,4-D) as auxins. Subculturing was done at a regular interval of 2 weeks in the same media having the same hormonal composition [11]. BAP, NAA and 2,4-D at the rates of 1, 2, 3 or 4 mg•L −1 were supplied to observe various stages of in vitro callus induction.…”