Micropropagation and conservation strategies of the potentially medicinal and economically-important tropical deciduous tree - Drypetes roxburghii (Wall.) Hurursawa

Murthy, K. Sri Rama; Reddy, Madhava C.

doi:10.5897/jmpr2014.5394

Cited by 3 publications

(2 citation statements)

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“…The increment in sucrose level enhanced the final germination percentage, with the highest value achieved by the standard sucrose concentration of MS medium (30 g/L). Improved seed germination using 30 g/L sucrose compared to low and high concentrations was also reported on another Acacia species, namely A. sieberiana [14], and on other tree species such as Prunus armeniaca [25], Boswellia serrata [26], and Drypetes roxburghii [27]. However, sucrose at 0-25 g/L revealed the same percentage of germination of Olea europaea [24].…”

Section: In Vitro Seed Germinationmentioning

confidence: 56%

“…Similarly, the reduction in MS salts in the cultivation medium was more efficient for the germination potential of Genipa americana seeds [28]. On the contrary, full-strength MS was better than other MS strengths for seed germination of A. sieberiana [14], Drypetes roxburghii [27], and Psoralea corylifolia [30]. The strengths of the MS medium also had an effect on the overall pattern of A. nubica seed germination (Figure 3).…”

Section: In Vitro Seed Germinationmentioning

confidence: 99%

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Germination and Early Growth of Acacia nubica Benth. In vitro

Ali¹,

Daffalla²,

Osman³

et al. 2023

ujps

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Acacia nubica is a well-adapted shrub to harsh conditions that provides valued socioeconomic and environmental advantages. In vitro culture of A. nubica is critical not only for investigating factors affecting cultivation conditions but also for producing juvenile tissue as a potential explants for various in vitro techniques. This research was aimed to study A. nubica seed germination and seedling growth in vitro in response to varied levels of sodium hypochlorite, sucrose, and Murashige and Skoog (MS) medium. The synchrony, percentage, and rate of germination of A. nubica seed were investigated. The shoot, root, and number of nodes were measured to gauge seedling development. Sodium hypochlorite at 10% resulted in the highest percentage of germination, whereas 5% resulted in a faster and more uniform emergence. Sucrose has various effects on seed emergence and germination depending on the concentrations; 30 g/L elicited the highest germination, while 10 g/L improved seed emergence rate with higher uniformity. Although ¼-MS medium produced the highest seed germination, 0-MS strength recorded faster and more synchronized germination. The development of A. nubica seedlings was influenced by sucrose concentrations and MS medium strengths. Sucrose at 20 g/L produced the maximum shoot length and vigor index, 30 g/L produced the maximum number of nodes, and 50 g/L produced the maximum root length and root/shoot ratio. Full MS medium supplemented with 30 g/L was shown to be the most effective in improving all assessed growth parameters. This is the first successful report on the seed germination and seedling growth of A. nubica in vitro.

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