Micropropagation and cytogenetic assessment of Zingiber species of Northeast India

Das, Ashmita; Kesari, Vigya; Rangan, Latha

doi:10.1007/s13205-012-0108-y

Cited by 24 publications

(10 citation statements)

References 18 publications

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“…Maximum frequency of shoot formation (78.3 %), with a mean of 9.3 shoots per explant was obtained on MS medium fortified with 8.0 µM BA. The positive effect of BA on shoot formation has also been reported in various plants such as Ajuga reptans (Preece and Huetteman 1994 ), ginger (Das et al 2013 ), Moringa oleifera (Saini et al 2012 ) and Scrophularia takesimensis (Jeong and Sivanesan 2015 ). Of the various concentrations of TDZ studied, maximum number of shoots (8.5) was obtained on MS medium fortified with 2.0 µM TDZ.…”

Section: Resultsmentioning

confidence: 69%

In vitro propagation, carotenoid, fatty acid and tocopherol content of Ajuga multiflora Bunge

et al. 2016

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The effect of plant growth regulators on shoot proliferation from shoot tip explants of Ajuga multiflora was studied. The highest number of shoots (17.1) was observed when shoot tip explants were cultured on Murashige and Skoog (MS) medium fortified with 8.0 µM 6-Benzyladenine (BA) and 2.7 µM α-naphthaleneacetic acid (NAA). The mean number of shoots per explant was increased 1.6-fold in liquid medium as compared with semi-solid medium. Maximum rooting (100 %) with an average of 7.2 roots per shoot was obtained on MS basal medium. Rooted plantlets were successfully acclimatised in the greenhouse with 100 % survival rate. Composition of carotenoids, fatty acids and tocopherols was also studied from leaves of greenhouse-grown plants and in vitro-regenerated shoots of A. multiflora. The greatest amounts of carotenoids, fatty acids and tocopherols were obtained from leaves of in vitro-regenerated shoots cultured on MS basal medium, followed by leaves of greenhouse-grown plants and leaves of in vitro-regenerated shoots cultured on MS basal medium with 2.0 µM BA or thidiazuron. The most abundant carotenoid in A. multiflora leaves was all-E-lutein (89.4–382.6 μg g−1 FW) followed by all-E-β-carotene (32.0–156.7 μg g−1 FW), 9′-Z-neoxanthin (14.2–63.4 μg g−1 FW), all-E-violaxanthin (13.0–45.9 μg g−1 FW), all-E-zeaxanthin (1.3–2.5 μg g−1 FW) and all-E-β-cryptoxanthin (0.3–0.9 μg g−1 FW). α-Tocopherol was the predominant tocopherol in A. multiflora leaves. Linolenic acid (49.03–52.59 %) was detected in higher amounts in A. multiflora leaf samples followed by linoleic acid (18.95–21.39 %) and palmitic acid (15.79–18.66 %).Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-016-0376-z) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 69%

In vitro propagation, carotenoid, fatty acid and tocopherol content of Ajuga multiflora Bunge

et al. 2016

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“…Profuse callus was produced at the basal cut end of shoots which inhibits the growth and elongation of roots when full strength MS medium was used. Half strength MS medium was preferred over full strength medium in many systems for root induction (Das et al 2013). Although we used three auxins (NAA, IBA and IAA) for root induction, the rooting response was optimum in IBA containing medium.…”

Section: Discussionmentioning

confidence: 99%

Plant regeneration from organogenic callus and assessment of clonal fidelity in Elephantopus scaber Linn., an ethnomedicinal herb

Abraham

Thomas

2015

Physiol Mol Biol Plants

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An efficient callus induction and plant regeneration system has been standardized for an ethnomedicinal plant, Elephantopus scaber Linn. Two explants i. e. seeds and leaf segments were used for callus induction. Murashige and Skoog (MS) medium supplemented with 5.0 μM 2, 4-dichlorophenoxy acetic acid (2, 4-D) and 0.5 μM kinetin (Kn) gave the optimum frequency (89 %) of callus induction from seed explant. The results showed that the highest response in terms of percent callus regenerating (91 %) and number of shoots (56) per culture was recorded on MS medium supplemented with 6.0 μM N6-benzylaminopurine (BA) and 1.5 μM α naphthalene acetic acid (NAA). The best rooting of regenerated shoots was obtained on half strength MS medium supplemented with 6.0 μM indole-3- butyric acid (IBA). On this medium, 100 % of the shoots produced roots with a mean number of 3.2 roots per shoot. The positive role of vesicular arbuscular mycorrhizae (VAM) along with potting mix has been well established in the present study. Of the various potting mix employed for plant acclimatization, the highest response of 100 % plant survival was noticed when autoclaved garden soil, sand (2:1) and VAM was utilized as potting mix. Inter-simple sequence repeats (ISSR) were used to establish the clonal fidelity of regenerated plantlets and the banding profiles from callus derived plants were monomorphic and similar to those of mother plant, thus ascertaining the true-to-type nature of these plants.

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“…4 Main procedures of acclimatization can be summarized as: 4 Remove plantlets with well-developed roots from the medium 4 Wash thoroughly under running tap water to remove adhering solid medium [21].…”

Section: Acclimatizationmentioning

confidence: 99%

A Review on Influence of Growth Regulator and Culture Condition on Micro- Propagation of Ginger (Zingiber officinale)

Mosie¹

2019

IJFSA

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Ginger is used in different medicinal systems of the world for a wide range of disorders. Commercially it is available in various forms, such as green ginger (fresh ginger), dry ginger, ginger powder, ginger oil, ginger oleoresin and preserved ginger. Now a day there is an outbreak of ginger wilt disease throughout the world which pushes researchers and experts to work on specifically;the application of plant tissue culture technique to produce disease free plant has been practiced. Thus the objective of the study is to review the influence of growth regulator and culture condition on micro propagation of ginger.Culture medium without growth regulator fails to stimulate the shoot bud initiation in the explants after four weeks of culture. Depending up on the type and concentration of growth regulators; number of days required for bud break varies from 6-10. Among the growth regulators, the medium containing BA (2.0-4.0 mgl) induced bud sprout in about 6-7 days. Auxin promotes the growth of intact roots and excised root sections but only at a relatively lower concentration range. If its concentration is high, it will suppress morphogenesis in cultured plants. The interaction of cytokinins and explant type had also significant differences for both leaf number and shoot length. The interaction of BAP and NAA produced high number of shoots (in the range of 14-19 shoots per explants) although BAP alone produced 12-13 shoots and 9 roots per explants.The influences of different growth regulators at different concentrations have important roles in promoting well developed roots. The pH condition of the medium should be adjusted to 5.8 before autoclaving at 1.04 kg/cm 2 pressure and 121°C temperature for 15-20 minute. All cultures should be incubated in 16 h light/8 h dark photoperiod (cool, white fluorescent light-30μ mol m-2 S-1) for successful production of plants.

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Micropropagation and cytogenetic assessment of Zingiber species of Northeast India

Cited by 24 publications

References 18 publications

In vitro propagation, carotenoid, fatty acid and tocopherol content of Ajuga multiflora Bunge

In vitro propagation, carotenoid, fatty acid and tocopherol content of Ajuga multiflora Bunge

Plant regeneration from organogenic callus and assessment of clonal fidelity in Elephantopus scaber Linn., an ethnomedicinal herb

A Review on Influence of Growth Regulator and Culture Condition on Micro- Propagation of Ginger (Zingiber officinale)

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