Micropropagation, Callus Induction and Regeneration of Ginger (<i>Zingiber officinale</i>Rosc.)

Miri, Seied Mehdi

doi:10.1515/opag-2020-0008

Cited by 20 publications

(7 citation statements)

References 18 publications

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“…Apart from BAP, kinetin [23] and thidiazuron (TDZ) [24] also differently influenced shoot multiplication of different varieties of ginger. In addition, a developed root system is the crucial step in plant vegetative propagation, which ensures a high survival rate of micropropagated plantlets during the acclimatization stage [25]. In vitro root induction of ginger is enhanced by auxins supplementation in the culture medium [26,27].…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Ginger (Zingiber officinale Roscoe) ‘Bentong’ and Evaluation of Its Secondary Metabolites and Antioxidant Activities Compared with the Conventionally Propagated Plant

Zahid

Jaafar

Hakiman

2021

Plants

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‘Bentong’ ginger is the most popular variety of Zingiber officinale in Malaysia. It is vegetatively propagated and requires a high proportion of rhizomes as starting planting materials. Besides, ginger vegetative propagation using its rhizomes is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied in many plant species to produce their disease-free planting materials. As ‘Bentong’ ginger is less known for its micropropagation, this study was conducted to investigate the effects of Clorox (5.25% sodium hypochlorite (NaOCl)) on explant surface sterilization, effects of plant growth regulators, and basal media on shoots’ multiplication and rooting. The secondary metabolites and antioxidant activities of the micropropagated plants were evaluated in comparison with conventionally propagated plants. Rhizome sprouted buds were effectively sterilized in 70% Clorox for 30 min by obtaining 75% contamination-free explants. Murashige and Skoog (MS) supplemented with 10 µM of zeatin was the suitable medium for shoot multiplication, which resulted in the highest number of shoots per explant (4.28). MS medium supplemented with 7.5 µM 1-naphthaleneacetic acid (NAA) resulted in the highest number of roots per plantlet. The in vitro-rooted plantlets were successfully acclimatized with a 95% survival rate in the ex vitro conditions. The phytochemical analysis showed that total phenolic acid and total flavonoid content and antioxidant activities of the micropropagated plants were not significantly different from the conventionally propagated plants of ‘Bentong’ ginger. In conclusion, the present study’s outcome can be adopted for large-scale propagation of disease-free planting materials of ‘Bentong’ ginger.

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Section: Introductionmentioning

confidence: 99%

Micropropagation of Ginger (Zingiber officinale Roscoe) ‘Bentong’ and Evaluation of Its Secondary Metabolites and Antioxidant Activities Compared with the Conventionally Propagated Plant

Zahid

Jaafar

Hakiman

2021

Plants

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“…Islam et al (2017) reported that the efficiency of shoot bud formation was relied upon and enhanced by supplementing the medium with BA. Similarly, Miri (2020) reported that the increase in BA concentration boosted the induction of axillary shoots of Zingiber officinale Rosc Furthermore, (Zhang et al, 2020) concluded that stem explant which grows on an MS medium containing 1.0mg L -1 BA without NAA giving high shoot multiplication to Malus sieversii.…”

Section: Shooting Regeneration Stagementioning

confidence: 92%

Antimicrobial and In-vitro Cytotoxic Efficacy of Betulinic Acid from the Callus, Regeneration, and Mother Plant of Dillenia indica L.

et al. 2021

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I N THIS STUDY, we established of an application protocol for the micropropagation of Dillenia indica with an estimate of betulinic acid from callus, leaf, and regeneration plant. Moreover, the antimicrobial and cytotoxic efficacy of the crude extract and pure compound was compared. The highest number of shoots (3.667 shoots), leaf number per jar (27 leaves) and shoot length (3.667cm) were recorded from the MS medium containing 2.0mg L -1 of benzyl amino purine (BAP). Moreover, different physical elicitors (ultraviolet irradiation, microwave radiation, and light quality) were used on the production and increased the concentration of betulinic acid in callus of D. indica. The data revealed that the highest betulinic acid content (341.775mg/ 100g fresh weight) was recorded in callus microwaved with 200 watts for 10s. Which was three times more than the betulinic acid content recorded in the mother plant. In addition, different crude extracts of D. indica were used to determine their biological activity against six microorganisms. The data showed that the highest diameter of inhibition zones (17.0 mm) was recorded in callus culture extractions incubated under blue light with Candida albicans. The cytotoxic efficacy of betulinic acid and extract of callus, mother plant leaf or in vitro regeneration plants of D. indica were investigated against the Vero cell line by MTT. The results revealed that The IC 50 values were 115.7 ± 9.17, 114.98 ± 5.2, 59.45 ± 3.06, and 72.76 ± 6.93μgmL -1 of betulinic acid, callus extract, leaf, and regeneration plant, respectively.

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“…Manokari et al (2016) also reported a reduction in H. sabdariffa shoot length from 26 mm to 11 mm with an increasing concentration (1.50-3 mg L -1 ) of BAP. High concentrations of BAP may cause ethylene production that negatively affects shoot regeneration and elongation by restricting lengthening of internodes (Davies et al, 2017;Miri, 2020). Callus formation (arrow) on all explants varied from 510 mm in diameter.…”

Section: Bap Concentration and The Type Of Explantmentioning

confidence: 99%

Preliminary study on in vitro shoot culture of Hibiscus coddii subsp. barnardii, an indigenous South African flowering plant

et al. 2021

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In vivo and in vitro grown plants of Hibiscus coddii subsp. barnardii were used as explant source for establishment of in vitro cultures. Nodal shoot explants derived from in vivo grown plants, both naturally and under controlled environmental conditions, showed high sensitivity to the surface disinfection treatment and poor survival in in vitro culture. In vitro grown seedlings proved successful as aseptic source of apical and basal shoot explants to establish contamination-free in vitro cultures. Sprouting of axillary buds was observed on 90% of apical shoot explants after four weeks of culture on full strength, plant growth regulator (PGR)-free Murashige and Skoog (MS) medium. However, further proliferation of short shoots, limited to the bud sprout at the explant base, occurred on only 50% of these explants. In contrast, all basal shoot explants attained 3-5 single primary axillary shoots (30-40 mm in length) while a clump of short (5-10 mm) shoots also formed at the base in 60% of these explants. In both explant types, addition of 0.25-1 mg L-1 6-Benzylaminopurine (BAP) to the MS medium resulted in a low frequency (10%-60%) of explants with short shoots (5-10 mm) that showed no further elongation. Moreover, explants cultured in the presence of BAP showed a high frequency of callus formation (up to 90%) and low survival (20%-60%). A lower frequency of callus formation (30%-40%) and higher survival (90%-100%) of both explant types occurred on BAP-free medium. Further subculturing of primary and secondary axillary shoots onto fresh MS medium (with and without BAP) did not improve shoot multiplication. Regenerated plantlets from PGR-free MS medium were successfully acclimatized and hardened-off.

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Micropropagation, Callus Induction and Regeneration of Ginger (Zingiber officinaleRosc.)

Cited by 20 publications

References 18 publications

Micropropagation of Ginger (Zingiber officinale Roscoe) ‘Bentong’ and Evaluation of Its Secondary Metabolites and Antioxidant Activities Compared with the Conventionally Propagated Plant

Micropropagation of Ginger (Zingiber officinale Roscoe) ‘Bentong’ and Evaluation of Its Secondary Metabolites and Antioxidant Activities Compared with the Conventionally Propagated Plant

Antimicrobial and In-vitro Cytotoxic Efficacy of Betulinic Acid from the Callus, Regeneration, and Mother Plant of Dillenia indica L.

Preliminary study on in vitro shoot culture of Hibiscus coddii subsp. barnardii, an indigenous South African flowering plant

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