Micropropagation of 4-year-old elite Eucalyptus tereticornis trees

Sharma, S. K.; Ramamurthy, V.

doi:10.1007/s002990050765

Cited by 58 publications

(31 citation statements)

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“…Com relação ao meio de cultura, o meio MS (MURASHIGE; SKOOG, 1962) tem sido um dos mais utilizados na micropropagação de Eucalyptus e seus híbridos (SHARMA; RAMAMURTHY, 2000;NUGENT et al, 2001;BILLARD;LALLANA, 2005;GLOCKE;COLLINS;SEDGLEY, 2006;AGGARWAL et al, 2012). No entanto, outros meios de cultura, como JADS (CORREIA et al, 1995), também têm sido empregados.…”

Section: Introductionunclassified

MULTIPLICAÇÃO E ALONGAMENTO IN VITRO DE CLONES HÍBRIDOS DE Eucalyptus globulus

Oliveira¹,

Xavier²,

Pontes‐Lopes³

et al. 2016

Ciênc. Florest.

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RESUMOOs híbridos de Eucalyptus globulus representam uma excelente alternativa para o setor de celulose e papel, em razão dos ganhos em qualidade da madeira para a fabricação de celulose. Entretanto, estes híbridos têm apresentado recalcitrância ao enraizamento adventício. Assim, a micropropagação é apontada como a técnica para o rejuvenescimento desses híbridos adultos, viabilizando a propagação clonal dos mesmos. O presente trabalho avaliou o cultivo in vitro de três clones de Eucalyptus grandis x Eucalyptus globulus e de três clones de Eucalyptus urophylla x Eucalyptus globulus, em relação à multiplicação in vitro, no meio MS suplementado com 0,5 mg L -1 de BAP e 0,01 mg L -1 de ANA, bem como o efeito das concentrações de 0,25; 0,50; 0,75 e 1,0 mg L -1 de AIB e dos meios de cultura MS e JADS no alongamento in vitro das brotações. Os clones diferiram quanto à multiplicação in vitro das brotações e apresentaram uma taxa de multiplicação média dos clones de 3,0 tufos de brotações em cada subcultivo, ao longo dos 25subcultivos realizados. No alongamento in vitro, os clones diferiram quanto às concentrações de AIB adequadas para provocar o alongamento, bem como em relação aos meios de cultura MS e JADS. O intervalo médio entre 0,40 e 0,80 mg L -1 de AIB proporcionou o maior número e comprimento das brotações alongadas in vitro e com maior vigor. Palavras-chave: rejuvenescimento; regulador de crescimento; meio de cultura; subcultivo. ABSTRACTEucalyptus globulus hybrids represent an excellent alternative to the pulp and paper sector, because of gains in wood quality for pulp. However, these hybrids have shown recalcitrance to adventitious rooting. Thus, micropropagation is considered the technique for rejuvenating adult genetic materials, allowing its clonal propagation. This study evaluated the in vitro culture of three clones of Eucalyptus grandis x Eucalyptus globulus and three clones of Eucalyptus urophylla x Eucalyptus globulus in relation to in vitro multiplication on MS medium supplemented with 0.

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Section: Introductionunclassified

MULTIPLICAÇÃO E ALONGAMENTO IN VITRO DE CLONES HÍBRIDOS DE Eucalyptus globulus

Oliveira¹,

Xavier²,

Pontes‐Lopes³

et al. 2016

Ciênc. Florest.

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“…Similar results in 'Safari Sunset' using a gelling agent were obtained by Pérez-Francés et al (1995). Any differences observed may be a result of the higher purity of phytagel and unidentified impurities in the agar (Sharma and Ramamurthy, 2000), which could contribute to inhibiting shoot growth (Hu and Wang, 1983). There are some reports of phytagel addition improving the multiplication phase in different plants (Saadat and Hennerty, 2002;Thimmappaiah and Sadhana, 2002).…”

Section: Discussionmentioning

confidence: 59%

Use of multinodal explants for micropropagation of Leucadendron ‘Safari Sunset’

Suárez

Pérez-Francés

Rodríguez-Pérez

2010

Span. j. agric. res.

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Leucadendron 'Safari Sunset' is one of the most valuable Proteaceae cultivar, due to its economic importance as cutflower. In vitro tissue culture of this cultivar and others may offer a massive cloned plant production that permit to satisfy the market demand. Multinodal explants of Leucadendron 'Safari Sunset' were established in vitro on a half strength Murashige and Skoog medium supplemented with 2.2 µM benzylaminopurine (BAP). Various antioxidant treatments were used to reduce the presence of phenolic compounds in culture. No important differences in growth and multiplication between these treatments were detected during in vitro establishment and multiplication, but explants cultured on a medium with 1 g L -1 of polyvinylpyrrolidone (PVP) developed a higher number of buds in the establishment phase. Proliferation of axillary shoots was achieved with 0.88 µM BAP, being slightly higher on a medium with 2.2 µM BAP although the buds were deformed, showing abnormal leaves. The use of phytagel in this phase increased proliferation rate and shoot growth. Low percentages rooting were obtained when microcuttings were cultured on the same basal medium supplemented with several indole-3-butyric acid (IBA) concentrations. The best rooting percentage (37.5%) was obtained with 44.1 µM IBA. This work shows that micropropagation of L. 'Safari Sunset' is possible with multinodal explants, although is necessary to optimize the protocol for commercial applications.Additional key words: antioxidant treatment, in vitro tissue culture, ornamental plants, phenolic compounds, Proteaceae. ResumenEmpleo de explantos multinodales para la micropropagación de Leucadendron 'Safari Sunset' Leucadendron 'Safari Sunset' es uno de los cultivares más importantes de Proteaceae debido a su importancia económica como flor cortada. El cultivo in vitro de este cultivar y otros, puede ofrecer una producción masiva de clones que permita satisfacer la demanda del mercado. Se establecieron in vitro explantos multinodales de L. 'Safari Sunset' en un medio Murashige y Skoog, con los macronutrientes a la mitad de su concentración, y bencilaminopurina (BAP) 2,2 µM. Para reducir la presencia de compuestos fenólicos en los cultivos, se emplearon diferentes tratamientos antioxidantes. No se observaron diferencias importantes en cuanto al crecimiento y multiplicación de las yemas, entre los antioxidantes empleados durante el establecimiento y multiplicación in vitro, sin embargo, se obtuvo un mayor nú-mero de yemas (25 ± 2,82) en los explantos sembrados en un medio con 1 g L -1 de polivinilpirrolidona (PVP). La multiplicación de las yemas se logró con BAP 0,88 µM. La tasa de proliferación fue ligeramente superior al adicionar al medio de cultivo BAP 2,2 µM, pero en este medio se desarrollaron yemas deformes, con hojas anormales. El empleo de fitagel incrementó la tasa de multiplicación y crecimiento de los vástagos. Se obtuvieron bajos porcentajes de enraizamiento al cultivar los microesquejes en el medio basal suplementado con diferentes concentraci...

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“…The highest mean number 32.73±0.14 of roots were obtained from 2.0 mg/L of IBA followed by 19.32±0.71 on (Iriondo et al, 1995), Asparagus robustus (Nayak and Sen 1998), Eucalyptus tereticornis (Sharma and Ramamurthy, 2000) and Hemides musindicus (Sreekumar et al, 2000).The root elongation phase is very sensitive to auxin concentration, and it is inhibited by high concentration of auxin in the rooting medium. Daffalla et al (2011) reported that roots may require a less concentration of auxin to grow, but root growth is strongly inhibited by its higher level because at this level, auxin induces the production of ethylene, a root growth inhibitor.…”

Section: Effect Of Different Concentrations Of Iba and Naa For In VImentioning

confidence: 99%

Micropropagation of Plectranthus edulis (Vatke) Agnew from shoot tip and nodal explants

Kebede¹,

Abera²

2015

Afr. J. Agric. Res.

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Plectranthus edulis Vatke belongs to the family of Lamiaceae, which occurs both as a wild and cultivated species. The major constraint in the cultivation of P. edulis is its low productivity due to shortage of planting materials and incidence of pests and diseases. In this study, an efficient protocol was established for the micropropagation of P. edulis germplasm using shoot tip and nodal explants. Explants were sterilized using different concentrations of Sodium hypochlorite (NaOCl) for different times of exposure. MS medium supplemented with different types and concentrations of auxin and cytokinin were used for culture initiation, shoot multiplication and root induction. NaOCl at a concentration of 2% and exposure time of 5 min gave 74.50±0.5% of clean culture for nodal and 69.83±0.76 from shoot tip. Six-Benzylaminopurine at 1.5 µM was found to be an optimum concentration for shoot induction, yielding 91.67±0.58% for nodal and 85.57±0.51% for shoot tip explants 3 weeks after culture. The combination of 2.0 µM BAP with 1.0 µM IAA was found to be the optimum concentration yielding 10.28±0.06 and 6.12±0.01 shoots per explants for nodal and shoot tip, respectively for shoot multiplication. Half strength MS medium with 2.0 µM IBA and 1.0 µM NAA gave the highest rooting percentage (97.00±0.28) with optimum root number (33) and length (3 cm). Up on acclimatization and transplanting, 83% survival efficiency was observed on soil mix ratio of 2:1:1 decomposed coffee husk, forest soil and sand, respectively. There were no observable variations with respect to morphology and growth characteristics to the greenhouse raised parent plants. The results obtained in this study permit the development of mass propagation protocol that could enable large scale commercial production of this highly demanded cultivar true-to type and provide a possible system towards genetic improvement of the crop.

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Micropropagation of 4-year-old elite Eucalyptus tereticornis trees

Cited by 58 publications

References 0 publications

MULTIPLICAÇÃO E ALONGAMENTO <i>IN VITRO</i> DE CLONES HÍBRIDOS DE<i> Eucalyptus globulus</i>

MULTIPLICAÇÃO E ALONGAMENTO <i>IN VITRO</i> DE CLONES HÍBRIDOS DE<i> Eucalyptus globulus</i>

Use of multinodal explants for micropropagation of Leucadendron ‘Safari Sunset’

Micropropagation of Plectranthus edulis (Vatke) Agnew from shoot tip and nodal explants

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