Micropropagation of a fruit tree, Morus australis Poir. syn. M. acidosa Griff.

Pattnaik, Swapnajit; Sahoo, Y.; Chand, Pradeep K.

doi:10.1007/bf00233153

Cited by 34 publications

(3 citation statements)

References 9 publications

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“…All these studies have reported the best multiplication of apical bud on MS basal medium supplemented with different concentrations of cytokinins only. Also, a similar situation was also noticed by Santamaria (Pattnaik et al, 1996). On the other hand, Gogoi et al (Gogoi & Borua, 2017) reported that supplementation of the MS medium with 1.5 mg/l of gibberelic acid (GA 3 ) improved elongated shoots of Morus indica L. (K2-cultivar).…”

Section: Discussionsupporting

confidence: 66%

Optimization of in vitro culture conditions affecting propagation of mulberry plant

et al. 2020

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Background: Mulberry Morus spp. L. Family Moraceae is a perennial tree crop cultivated worldwide for foliage, fruit, fuel wood, and medicine, as well as for several other sericulture and industrial purposes. Mulberry trees are grown in more than 50 countries covering temperate, subtropical, and tropical zones as a plantation crop. Methodology: In this study, different stages of in vitro propagation mulberry plant were investigated. In the sterilization stage, different concentrations 0.1, 0.2, and 0.3 (%) of mercuric chloride (MC) for 10 min were used. Also, the effect of supplementation of the Murashige and Skoog (MS) medium with different concentrations 0, 0.5, 1.0, and 1.5 mg/l of BAp or TDZ on the multiplication stage was investigated. Moreover, the impact of the fortified MS medium with 1 mg/l IAA or NAA or IBA on enhancement of root system proliferation was studied. Furthermore, the effect of reculturing in vitro derived plantlets on peatmoss, perlite, and/or vermiculate for in vivo acclimatization and hardening was investigated.Results: A simple and efficient protocol for in vitro propagation of different varieties of Morus alba (yue 11, sha 2x lun 109, morittina, kokuso 27, and kantava 2) was established. Sterilization of shoot tips and auxiliary buds with 0.2% of mercuric chloride (MC) for 10 min was the best to get sterilized and survival explants. In vitro mulberry multiplication of yue 11, sha 2x lun 109, morittina, kokuso 27, and kantava auxiliary buds cultured on the MS medium fortified with 1.5 mg/l BA gave the best results of the multiplication rates, maximum number of shoots/ explant, and highest shoot length (cm), respectively. Moreover, culturing of derived mulberry shoots on the MS medium supplemented with 1 mg/l NAA enhanced totally growth figures of root system formation suitable for acclimatization and hardening of in vitro derived mulberry plants. Acclimatization and hardening successfully processed with canava 2, morittina, yue 11, sha2x, and kokuso of mulberry cultivars, respectively. Conclusion:The described protocol in this investigation ensures a round-the-year in vitro propagation of mulberry plant with faster rate of multiplication of true-to-type plants, which will provide a useful tool for large-scale multiplication of other mulberry varieties.

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Section: Discussionsupporting

confidence: 66%

Optimization of in vitro culture conditions affecting propagation of mulberry plant

et al. 2020

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“…However, further increase in AdSO 4 inhibited all three of the responses mentioned above and the level of inhibition was proportional to the increase in the concentration of AdSO 4 (Table 1). Pattnaik et al (1996), in their experiments with Morus australis micropropagation, have also noticed that higher concentrations of cytokinins elicited inhibitory effects on shoot elongation. The basal medium supplemented with 4.92 mM IBA induced a higher percentage of rooting (Fig.…”

Section: Resultsmentioning

confidence: 92%

In vitro propagation of the medicinal plant Plumbago zeylanica L. Through nodal explants

Selvakumar

Anbudurai

Balakumar

2001

In Vitro Cell.Dev.Biol.-Plant

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A protocol for rapid in vitro propagation using nodal explants obtained from 2-yr-old, field-grown medicinal plants of Plumbago zeylanica L. belonging to the family Plumbaginaceae is described. High frequency bud break and fast development of shoots were induced on Murashige and Skoog's basal medium supplemented with 27.2 mM adenine sulfate +2.46 mM indole-3-butyric acid (IBA). Induction of rooting was achieved by transferring the shoots to the same basal medium containing 4.92 mM IBA. Using our protocol from one twig of P. zeylanica (eight responsive nodes per explant shoot) within a period of 5 mo., eight plantlets could be raised. After a hardening period of 4 wk, there was a 90% transplantation success in the field compared to the 60±65% survival of plantlets recorded in the experiments of previous workers. The plantlets derived through in vitro propagation mimic the growth and morphological characteristics of the donor plants.

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“…A reduction in the number of shoots higher than the optimal level was earlier reported in other medicinal plants including Capsicum annum L. (Ahmad et al, 2006). Higher concentration of cytokinin elicited inhibitory effects on shoot elongation was also earlier reported by Pattnaik et al (1996). Regenerated leafy shoots derived from the nodal segments are introduced to triple auxins combinations for rooting.…”

Section: Resultsmentioning

confidence: 52%

Development of a simplified protocol for in vitro propagation of a valuable medicinal plant Plumbago zeylanica Linn. through nodal explants found in Odisha, India

Kanungo¹

2012

J. Med. Plants Res.

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An efficient and economic protocol for rapid in vitro propagation using nodal explants obtained from 2 year old, field grown medicinal plants of Plumbago zeylanica L. belonging to the family Plumbaginaceae was successfully achieved from the nodal segments. Shoot development was maximum (95%) on Murashige and Skoog's (MS) basal medium supplemented with 6-Benzyl amino purine (BAP) (2.0 mg/l), indole-3-acetic acid (IAA) (1.5 mg/l) and indole-3-butyric acid (IBA) (1.0 mg/l) with (19.56±0.04) mean number of shoots per explants and the maximum shoot length was found to be (4.98±0.87). Rooting of the differentiated shoots was achieved in MS medium with triple auxins combination of αnaphthalene acetic acid (NAA) (1.5 mg/l), IAA (1.5 mg/l) and IBA (2.0 mg/l) with (18.54±0.09) mean number of roots per shoots and the mean root length was found to be (7.63±0.83). Regenerated plantlets were successfully acclimated in the green house and after a hardening period of 4 weeks 100% transplantation success was achieved under the natural condition. The plantlets derived through in vitro propagation mimic the morphological characteristics of the donor plants taken for the present investigation.

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Micropropagation of a fruit tree, Morus australis Poir. syn. M. acidosa Griff.

Cited by 34 publications

References 9 publications

Optimization of in vitro culture conditions affecting propagation of mulberry plant

Optimization of in vitro culture conditions affecting propagation of mulberry plant

In vitro propagation of the medicinal plant Plumbago zeylanica L. Through nodal explants

Development of a simplified protocol for in vitro propagation of a valuable medicinal plant Plumbago zeylanica Linn. through nodal explants found in Odisha, India

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