Micropropagation of an Indian Ginger (Curcuma vamana Sabu and Mangaly): A Wild Relative of Turmeric

Bejoy, M.; Dan, Mathew; Anish, N.P.; Nair, Anjana R G; Radhika, B; Manesh, K.

doi:10.3923/biotech.2012.333.338

Cited by 11 publications

(3 citation statements)

References 12 publications

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“…The effect of cytokinins on shoot multiplication is presented in Table 1. The notable efficiency of BAP in inducing effective multiplication aligned with findings from other micropropagation studies carried out on Curcuma species like C. haritha (Bejoy et al 2006), C. mangga (Raihana et al 2011), C. caesia (Bharalee et al 2005), C. zedoaria (Loc et al 2005), C. vamana (Bejoy et al 2012), etc.…”

Section: Initiation Of Culture and Multiplicationsupporting

confidence: 82%

6 – BENZYLAMINOPURINE INDUCES HIGH-FREQUENCY MULTIPLICATION IN VULNERABLE Curcuma pseudomontana J. Graham.: A POTENTIAL EX VIVO CONSERVATION TOOL

Vaze,

Pramod Hurkadale,

Hegde

2024

BIOTROPIA

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A rapid high-frequency multiplication protocol is designed for Curcuma pseudomontana J. Graham, belonging to the family Zingiberaceae, an endemic species to the Western Ghats of India. The IUCN Red List of Threatened Taxa mentions this species as vulnerable due to multiple underlying causes. The Plant is extensively used in traditional and tribal medicine. The species has suffered habitat loss due to uncontrolled use for tribal medicine leading to a 30% loss in the last decade. This study is planned with a specific objective to conserve the species, and this is the first-ever report of micropropagation of Curcuma pseudomontana J. Graham using in vitro multiplication. An efficient rapid protocol for Micropropagation is developed using rhizome bud explants. The explants are transferred from MS basal medium onto the MS medium fortified with BAP, KN, TDZ at a varying concentration range. The maximum shoot induction is observed in MS medium enriched with BAP 2mg L-1resulting in 9.66 ±2.08 number of shoots per explant with a shoot length of 6.40 ±0.36cm. The root induction response is studied by aseptically transferring the shoots onto MS medium fortified with NAA, IBA, and IAA at varying concentration. Maximum root length and root number is recorded in MS supplemented with 1- Naphthalene Acetic Acid (NAA) at 0.5 mg L-1. However, a 100 % root induction frequency is observed in all the samples under study. The rooted plantlets are removed from the culture flasks and transferred into hardening media containing 1:1:1 ratio of Sand: Soil: Cocopeat. The hardened plants are healthy and disease-free and showed a 92% survival after acclimatization.

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Section: Initiation Of Culture and Multiplicationsupporting

confidence: 82%

6 – BENZYLAMINOPURINE INDUCES HIGH-FREQUENCY MULTIPLICATION IN VULNERABLE Curcuma pseudomontana J. Graham.: A POTENTIAL EX VIVO CONSERVATION TOOL

Vaze,

Pramod Hurkadale,

Hegde

2024

BIOTROPIA

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“…Different basal media effects showed that MS and LS media were more effective for shoot multiplication of ‘Bentong’ ginger than B5 medium. Bejoy et al [ 41 ] also found that MS medium was more effective than B5 medium for shoot multiplication of Curcuma vamana . The diverse effects of different basal media could be due to the different ratios of the two nitrogen sources (ammonium and nitrate ions) in their composition [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Micropropagation of Ginger (Zingiber officinale Roscoe) ‘Bentong’ and Evaluation of Its Secondary Metabolites and Antioxidant Activities Compared with the Conventionally Propagated Plant

Zahid

Jaafar

Hakiman

2021

Plants

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‘Bentong’ ginger is the most popular variety of Zingiber officinale in Malaysia. It is vegetatively propagated and requires a high proportion of rhizomes as starting planting materials. Besides, ginger vegetative propagation using its rhizomes is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied in many plant species to produce their disease-free planting materials. As ‘Bentong’ ginger is less known for its micropropagation, this study was conducted to investigate the effects of Clorox (5.25% sodium hypochlorite (NaOCl)) on explant surface sterilization, effects of plant growth regulators, and basal media on shoots’ multiplication and rooting. The secondary metabolites and antioxidant activities of the micropropagated plants were evaluated in comparison with conventionally propagated plants. Rhizome sprouted buds were effectively sterilized in 70% Clorox for 30 min by obtaining 75% contamination-free explants. Murashige and Skoog (MS) supplemented with 10 µM of zeatin was the suitable medium for shoot multiplication, which resulted in the highest number of shoots per explant (4.28). MS medium supplemented with 7.5 µM 1-naphthaleneacetic acid (NAA) resulted in the highest number of roots per plantlet. The in vitro-rooted plantlets were successfully acclimatized with a 95% survival rate in the ex vitro conditions. The phytochemical analysis showed that total phenolic acid and total flavonoid content and antioxidant activities of the micropropagated plants were not significantly different from the conventionally propagated plants of ‘Bentong’ ginger. In conclusion, the present study’s outcome can be adopted for large-scale propagation of disease-free planting materials of ‘Bentong’ ginger.

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“…Shoot proliferation is an essential step for mass production of in vitro plantlets which is normally car-ried out by inducing multiple shoots formation using plant growth regulators. Many researchers have been using 6-benzylaminopurine (BAP) or benzyl adenine (BA) combination with naphthalene acetic acid (NAA) for multiple shoot induction in Zingiber species (Abbas et al, 2011;Zahid et al, 2021) and Curcuma species (Bejoy et al, 2012;Jala, 2012;Ferrari et al, 2016) with the formation of an average of 3 to 5 shoots per explants. Similar result was obtained in the present study whereby an average of 3.3 and 3.7 shoots per shoot explant were formed when the shoot explants were cultured horizontally and vertically respectively on MS medium supplemented with 2.0 mg l -1 BA and 0.5 mg l -1 NAA for induction of multiple shoot formation.…”

Section: Effects Of Shoot Incision On Shoot Proliferationmentioning

confidence: 99%

Enhancement of shoot proliferation and evaluation of biotic elicitation effects on anatomical changes of pseudo stem and anti-lipid peroxidation activity of Curcuma mangga Val.

Abraham

Chan

Indrayanto

et al. 2022

AAS

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Mango turmeric (Curcuma mangga Val.) contains many bioactive compounds that are used for traditional treatment of various health problems and ailments. Slow propagation nature of C. mangga have resulted in short supply to meet the market demand. The longitudinally incised half shoot explants promote 100 % increased of shoot number compared with non-incised shoots with the formation of average 6.6 shoots/explant when they were cultured either vertically or horizontally on MS medium supplemented with 2.0 mg l-1 BA and 0.5 mg l-1 NAA. Biotic elicitation with 3.5 mg l-1 or 5.0 mg l-1 yeast extract or combination of 150 mg l-1 chitosan and 3.5 mg l-1 yeast extract did not promote shoot proliferation but exhibited anti-lipid peroxidation activity slightly lower than quercetin, a potent plant antioxidant flavonoid and butyl hydroxyl toluene (BHT), a commercial preservative agent which is used as a positive control. While absolute ethanol which served as a negative control did not show any anti-lipid peroxidation activity. Biotic elicitation of C. mangga plantlets using similar elicitors resulted in anatomical changes of its pseudostem with reduced number of thin lignified xylem cells and the presence of druse suspected to be oxalate crystals inside the cortex cells with delicate cell wall.

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Micropropagation of an Indian Ginger (Curcuma vamana Sabu and Mangaly): A Wild Relative of Turmeric

Cited by 11 publications

References 12 publications

6 – BENZYLAMINOPURINE INDUCES HIGH-FREQUENCY MULTIPLICATION IN VULNERABLE Curcuma pseudomontana J. Graham.: A POTENTIAL EX VIVO CONSERVATION TOOL

6 – BENZYLAMINOPURINE INDUCES HIGH-FREQUENCY MULTIPLICATION IN VULNERABLE Curcuma pseudomontana J. Graham.: A POTENTIAL EX VIVO CONSERVATION TOOL

Micropropagation of Ginger (Zingiber officinale Roscoe) ‘Bentong’ and Evaluation of Its Secondary Metabolites and Antioxidant Activities Compared with the Conventionally Propagated Plant

Enhancement of shoot proliferation and evaluation of biotic elicitation effects on anatomical changes of pseudo stem and anti-lipid peroxidation activity of Curcuma mangga Val.

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