Micropropagation of Arid Zone Fruit Tree, Pomegranate, cvs. ‘Malase Yazdi’ and ‘Shirine Shahvar’

Mulaei, Sara; Jafari, Azam; Shirmardi, Mostafa; Kamali, Koorosh

doi:10.1080/15538362.2019.1680334

Cited by 8 publications

(9 citation statements)

References 26 publications

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“…The results obtained in that work 23 were higher than others. Mulaei et al 24 achieved the highest rate (90.58%) of survival of axillary buds using 0.1% HgCl 2 solution for 10 min. Similar results were obtained in our work.…”

Section: Resultsmentioning

confidence: 98%

Optimization of Development of Pomegranate (Punica granatum L.) Varieties from Microclonal Propagation

Ubaydullae¹,

Bolkiev²,

Abdullaev³

et al. 2023

Asian J. of Plant Sciences

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Background and Objective: Besides, increasing cost-efficiency of MS and WPM nutrient media for the regeneration of explants and rhizogenesis of microshoots was planned by introducing changes in the composition of phytohormones. Studying the effect of a supramolecular complex of glycyrrhizinic and salicylic acids on the rhizogenesis of microshoots was aimed in terms of economic benefits. The research was aimed at optimizing in vitro microclonal propagation of some pomegranate varieties (Punica granatum L.).Materials and Methods: August, Kazake-anar, Achikh-dona and Tuyatish varieties of local pomegranate were selected in the study.Various concentrations of sterilants were used for the surface sterilization of explants. Explants were grown in MS and WPM media containing various concentrations of cytokinin, auxin and gibberellic acid. The supramolecular complex was used for microshoot rhizogenesis. Moreover, optimal conditions for acclimatization in microplants were developed. Results: The addition of gibberellic acid into MS and WPM nutrition media did not result in significant differences in the microshoot length. But the addition speeded up the formation of buds. Only earlier development of buds in some varieties were observed. The supramolecular complex used in this work at 0.15 and 0.17 mg LG 1 led to several-fold improvement in root number and length. No significant changes were observed between MS and WPM culture media. Economically utilizing the supramolecular complex was about 45 times cheaper than the mixture of IBA and IAA that was added to MS and WPM nutrition media. Conclusion: An enhanced cost-efficiency of the technology that enables obtaining pomegranate microshoot during the whole year.

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Section: Resultsmentioning

confidence: 98%

Optimization of Development of Pomegranate (Punica granatum L.) Varieties from Microclonal Propagation

Ubaydullae¹,

Bolkiev²,

Abdullaev³

et al. 2023

Asian J. of Plant Sciences

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“…Plant tissue culture methods offer a promising approach for the rapid production of true-to-type pomegranate plants and the biotechnological exploitation of pomegranate and other plant species with valuable properties [5]. Previous studies have attempted to apply in vitro culture techniques to propagate different cultivars of pomegranate [6,7]. However, the findings have clearly emphasized that pomegranate micropropagation is moderately difficult and can vary depending on the cultivar, probably due to genetic variations among them [6,8].…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have attempted to apply in vitro culture techniques to propagate different cultivars of pomegranate [6,7]. However, the findings have clearly emphasized that pomegranate micropropagation is moderately difficult and can vary depending on the cultivar, probably due to genetic variations among them [6,8]. Nevertheless, the successful propagation of economically important woody plant species like pomegranate still presents challenges, due to the emergence of some problems during the proliferation stage including defoliation of explants, shoot tip necrosis, callusing, and hyperhydricity.…”

Section: Introductionmentioning

confidence: 99%

Optimizing PGRs for in vitro shoot proliferation of pomegranate with bayesian-tuned ensemble stacking regression and NSGA-II: a comparative evaluation of machine learning models

Zarbakhsh,

Shahsavar,

Soltani

2024

Plant Methods

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Background The process of optimizing in vitro shoot proliferation is a complicated task, as it is influenced by interactions of many factors as well as genotype. This study investigated the role of various concentrations of plant growth regulators (zeatin and gibberellic acid) in the successful in vitro shoot proliferation of three Punica granatum cultivars (‘Faroogh’, ‘Atabaki’ and ‘Shirineshahvar’). Also, the utility of five Machine Learning (ML) algorithms—Support Vector Regression (SVR), Random Forest (RF), Extreme Gradient Boosting (XGB), Ensemble Stacking Regression (ESR) and Elastic Net Multivariate Linear Regression (ENMLR)—as modeling tools were evaluated on in vitro multiplication of pomegranate. A new automatic hyperparameter optimization method named Adaptive Tree Pazen Estimator (ATPE) was developed to tune the hyperparameters. The performance of the models was evaluated and compared using statistical indicators (MAE, RMSE, RRMSE, MAPE, R and R2), while a specific Global Performance Indicator (GPI) was introduced to rank the models based on a single parameter. Moreover, Non‑dominated Sorting Genetic Algorithm‑II (NSGA‑II) was employed to optimize the selected prediction model. Results The results demonstrated that the ESR algorithm exhibited higher predictive accuracy in comparison to other ML algorithms. The ESR model was subsequently introduced for optimization by NSGA‑II. ESR-NSGA‑II revealed that the highest proliferation rate (3.47, 3.84, and 3.22), shoot length (2.74, 3.32, and 1.86 cm), leave number (18.18, 19.76, and 18.77), and explant survival (84.21%, 85.49%, and 56.39%) could be achieved with a medium containing 0.750, 0.654, and 0.705 mg/L zeatin, and 0.50, 0.329, and 0.347 mg/L gibberellic acid in the ‘Atabaki’, ‘Faroogh’, and ‘Shirineshahvar’ cultivars, respectively. Conclusions This study demonstrates that the 'Shirineshahvar' cultivar exhibited lower shoot proliferation success compared to the other cultivars. The results indicated the good performance of ESR-NSGA-II in modeling and optimizing in vitro propagation. ESR-NSGA-II can be applied as an up-to-date and reliable computational tool for future studies in plant in vitro culture.

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“…[6] they found that MS medium was better than WPM medium for the regeneration and growth of explant [7] revealed that culturing on basal MS medium supplement with 0.5 mg.l -1 BAP was effective for shoot initiation while [8] found that WPM medium was more proper for culturing of vitis thanbergii than MS. [9] found WPM supplemented with 1mg.l (BAP) produced maximum number of shoot induction and highest number of roots and root length. [1] reported that WPM be more efficient compared to MS was gave highest number of nodes shoot length and leaf number, on the other hand [10] found explants like shoot tips and internode segments have been used in the tissue culture studies [11] found that internode segments explants were best than the other parts for shoot proliferation and the other micro propagation stages. Cytokinines play important role in the multiplication stage which have an important role in cell division and cellular differentiation.…”

Section: Introductionmentioning

confidence: 99%

Effect of Media Type and Cytokinines on Micropropagation of Two Cultivars of Pomegranate Punica granatum L

Abdulkareem

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Present study was done to describe micropropagation protocol of Pomegranate (granatum Punica L) for two cultivar (Yamani and Wonderful), by testing their tolerance to sterilization duration with sodium hypochlorite solution at 1%, and investigation of suitability of of two media murashige and skoog (MS) and woody plant medium (WPM) during establishment stage in addition study the role of four type of Cytokinines BA (benzyl adenine), 2iP (isopentyl adenine), Kinetin(6-furfury lamino Purine) and TDZ (Thidiazuron) at different concentrations for two cultivars in multiplication stage and investigation of two type of Auxin NAA and IBA for rooting stage. the results appeared that sterilization at 10 min with sodium hypochlorite at 1% reduce contamination percentage to 6.2% and increased survival to 93.8%, the result proved WPM media was better than MS in increase shoot number, shoot length and leaf number was 1.17 shoot per explant, 5.02cm and 6.60 leaf per explant respectively. in the other way Yamani cultivar gave maximum value in comparison with wonderful for same characteristics. concerning the multiplication stage at 0.5 mg.l-1 BA achieved highest value of shoot number and leaf number (2.89 and 5) per explant respectively. while Kin gave best value of shoot length 4.12cm. moreover BA and 2iP at 1mg.l-1, Kin at 5mg.l-1 and TDZ at1mg.l-1 achieved maximum values of same characteristics mentioned above. Auxins NAA (Naphthaleneacetic acid) and IBA (Indole-3-butyric acid) at 1mg.l-1 achieved highest root number was (4 and 3.17) per explant respectively.

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Micropropagation of Arid Zone Fruit Tree, Pomegranate, cvs. ‘Malase Yazdi’ and ‘Shirine Shahvar’

Cited by 8 publications

References 26 publications

Optimization of Development of Pomegranate (Punica granatum L.) Varieties from Microclonal Propagation

Optimization of Development of Pomegranate (Punica granatum L.) Varieties from Microclonal Propagation

Optimizing PGRs for in vitro shoot proliferation of pomegranate with bayesian-tuned ensemble stacking regression and NSGA-II: a comparative evaluation of machine learning models

Effect of Media Type and Cytokinines on Micropropagation of Two Cultivars of Pomegranate Punica granatum L

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