Micropropagation of Asparagus (Asparagus officinalis L.)

Desjardins, Yves

doi:10.1007/978-3-662-07770-2_2

Cited by 8 publications

(4 citation statements)

References 39 publications

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“…The main differences between the ARBM-0 and ARBM-3 cultures medium are the presence of ancymidol and a higher sucrose concentration (60 g l -1 ) in the latter medium (Table 3). The ancymidol is a growth retardant that, combined with high doses of sucrose, improves rooting and plantlet development in asparagus (Desjardins et al 1987;Desjardins 1992;Carmona-Martin et al 2014). In the work cited above, the explants were continuously cultured on the medium with ancymidol and high doses of sucrose.…”

Section: Discussionmentioning

confidence: 99%

Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs

Regalado

Martín

Castro

et al. 2015

Plant Cell Tiss Organ Cult

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An efficient micropropagation method for asparagus species was developed in this study. The method allows the fast cloning of the elite genotypes from different asparagus species and the interspecific hybrids obtained from these species. Rhizome bud explants were disinfected using 3 g l -1 of benomyl and 20 g l -1 of sodium hypochlorite. Then, they were cultured on Asparagus Rhizome Bud Medium 3 (ARBM-3) consisting in modified Murashige and Skoog medium with salts with EDDHA-Fe (85.7 mg l -1 ) instead of EDTA-Fe and vitamins, supplemented with 0.3 mg l -1 NAA, 0.1 mg l -1 KIN, 2 mg l -1 ancymidol and 6 % sucrose. Results showed that the method developed produced high disinfection rates (70-95 %). More than 70 % of the explants developed shoots and the rooting rate on ARBM-3 medium was 30-45 %. The rooting rate increased to 60-85 % when the unrooted shoots were subjected to an additional cycle of rooting, reaching 100 % after two cycles of rooting. The multiplication was achieved through mechanical division of rooted shoot clusters growing in ARBM-3. The acclimatization rate of the micropropagated plantlets was higher than 90 %. The micropopagated plantlets were screened for somaclonal variation using 12 expressed sequence tag derived simple sequence repeat markers. The results confirmed the character ''true to type'' of the plantlets, indicating that the method developed in this study can be used to successfully micropropagate asparagus species.

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Section: Discussionmentioning

confidence: 99%

Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs

Regalado

Martín

Castro

et al. 2015

Plant Cell Tiss Organ Cult

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“…Phương pháp này có nhiều hạn chế như hệ số nhân giống thấp, không đảm bảo sạch bệnh. Phương pháp nhân giống bằng hạt khó thực hiện với người nông dân vì Măng tây là cây phân tính khác thân dị hợp tử, việc thụ phấn tạo hạt khó tiến hành [5]. Do đó, hạt giống Măng tây thường được sản xuất bởi các đơn vị chuyên môn, tuy nhiên hạt có tỉ lệ nảy mầm không cao và giá thành đắt đỏ nên việc cung cấp giống Măng tây gặp nhiều khó khăn.…”

Section: Mở đầUunclassified

NGHIÊN CỨU MỘT SỐ ĐIỀU KIỆN NUÔI CẤY MĂNG TÂY (Asparagus officinalis) IN VITRO

Yến¹,

Sắc²

2022

TNUJST

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Măng tây (Asparagus officinalis L.) là loại rau cao cấp có hàm lượng dinh dưỡng cao, ngoài ra còn có tác dụng làm hoa cảnh. Nghiên cứu nhân giống in vitro Măng tây để cung cấp nguồn con giống đồng đều, sạch bệnh và giảm chi phí đáp ứng nhu cầu giống ngày càng cao là rất cần thiết. Trong nghiên cứu này, các yếu tố ảnh hưởng đến quá trình tạo mẫu sạch bệnh, sự phát sinh chồi, tái sinh và phát triển chồi trong nuôi cấy Măng tây in vitro đã được khảo sát. Kết quả cho thấy, khử trùng hạt măng tây bằng HgCl2 0,1% trong 20 phút cho kết quả tốt nhất, tỉ lệ hạt sống nảy mầm không nhiễm bệnh đạt 70,83%; Môi trường Murashige và Skoog bổ sung 20 gL-1 sucrose; 2 mg L-1 BAP và 15% v/V nước dừa thích hợp cho sự phát sinh chồi từ hạt (tỉ lệ nảy mầm đạt 90% với chiều cao trung bình 3,27 ± 0,35 cm sau 20 ngày nuôi cấy); Kích thích sinh trưởng thích hợp cho nhân chồi Măng tây in vitro là phối hợp BAP 2 mg L-1 với NAA 0,3 mg L-1. Môi trường Murashige và Skoog bổ sung sucrose 30 L-1; BAP 2 mg L-1 và NAA 0,3 mg L-1 cho hệ số nhân chồi cao nhất, đạt 6,55 lần, các chồi có chất lượng tốt, to khỏe, xanh đậm.

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“…Several authors pointed out that major obstacle of Asparagus micropropagation protocols is not the establishment and multiplication of shoots, but root initiation. None of the existing pathways for large commercial scale propagation of Asparagus is useful, as regenerated plantlets have poor survival hardening rate (Desjardins, 1992). Asparagus plants require vigorously growing roots for successful transplantation, the obstacles of Asparagus rooting in vitro can be categorized into three aspects: 1. low rooting rate, 2. big mount of abnormal roots, 3. low survival rate after transplanting to the greenhouse (Ren et al, 2012).…”

mentioning

confidence: 99%

In Vitro Propagation of F1 Male Hybrid Lines in Asparagus Officinalis

Sallam

2019

Egyptian Journal of Desert Research

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or mass production of super male hybrid lines of Asparagus officinalis (Mary Washington 500 W' CV.), in vitro propagation system was initiated to obtain a sterile, healthy culture using shoot tip and single-node spear. Explants established on Murashige and Skoog (MS) medium supplemented with kinetin (KIN) at 1 mg l-1 , naphthalene acetic acid (NAA) at 0.2 mg l-1 and gibberellic acid (GA3) at 0.5 mg l-1 showed 100% of survival, the highest number of thick, dark green shoots (8.5) and nodes (8.7). Multiplication of shoots was carried out using benzyl amino purine (BAP) or KIN. BAP containing media gave higher shoot multiplication rate with high vitrification ratio in a positive correlation with concentration. The vitrification ratio was declined by replacing BAP with the same concentration of KIN (2 mg l-1). Adding the growth retardant; paclopatrazol (PP333) at 1.0 mg l-1 completely eliminated the vitrification. Direct somatic embryos from stem segment (internode) induced on MS medium supplemented with BAP at 1 mg l-1 and NAA at 0.1 mg l-1 and seedling regeneration occurred on MS medium with KIN at 1 mg l-1 and GA3 at 0.5 mg l-1. Medium supplemented with NAA, indole-3-butyric acid (IBA), PP333 and Aspergillus niger extract were tested for rooting. MS medium supplemented with IBA at 1.0 mg l-1 and PP333 at 0.5 mg l-1 was optimum for producing white-rooted plants (79.5%) within 54 days from old clusters of shoots, which were better, compared to young single shoots. Healthy rooted clusters from each treatment were successfully transferred to growth champers then greenhouse for acclimatization.

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Micropropagation of Asparagus (Asparagus officinalis L.)

Cited by 8 publications

References 39 publications

Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs

Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs

NGHIÊN CỨU MỘT SỐ ĐIỀU KIỆN NUÔI CẤY MĂNG TÂY (Asparagus officinalis) IN VITRO

In Vitro Propagation of F1 Male Hybrid Lines in Asparagus Officinalis

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