Micropropagation of black pepper (Piper nigrum Linn.) through shoot tip cultures

Abstract

The morphogenetic potential of shoot tip explants of black pepper (Piper nigrum) was investigated and an effective multiple-shoot propagation method is described. Various combinations of media, growth regulators and sterilization treatments were compared. Problems with establishment in tissue culture sometimes occurred, probably caused by endogenous pathogens associated with tissue exudates. The best establishment and proliferation of shoot tip explants was obtained on MS medium containing 1.5 mg l(-1) BAP alone; subsequent growth and development of lateral branches was best on media containing 1.5 mg l(-1) BAP plus 3.0 mg l(-1) IBA. Adenine sulphate inhibited the number of explants showing regeneration but increased the number of shoot buds per regenerating explant. Shoots were rooted on a 50% strength medium containing 1mg l(-1) NAA.

In vitro plant regeneration system for common bean (Phaseolus vulgaris): effect of N6-benzylaminopurine and adenine sulphate

“…Deshpande et al (1998) indicated that a low concentration of cytokinins in combination with AS favors multiple shoot induction in Ficus religiosa. In black pepper (Piper nigrum), Philip et al (1992) reported that AS increased the number of shoot buds per regenerating explant.…”

Concentrações de citocinina e carvão ativado na micropropagação de pimenta-do-reino

“…PHILIP et al (1992) também obtiveram a multiplicação in vitro de pimenta-do-reino a partir de ápices caulinares de plantas adultas. Um dos problemas em obterem-se protocolos de regeneração in vitro a partir de plantas adultas é o controle das taxas de contaminação vindas do campo.…”

“…Isso confirma que concentrações menores de BAP são mais eficientes na indução de brotações e formação de explantes para "Bragantina". Entretanto, isso parece ser variável entre os genótipos de pimenta-do-reino, já que PHILIP et al (1992) verificaram que a concentração de 1,5mg L -1 foi mais eficiente que concentrações mais baixas de BAP na indução de brotos de pimenta-do-reino de uma variedade indiana.…”

“…Protocolos de regeneração in vitro para a pimenta-do-reino já foram descritos, (PHILIP et al, 1992;BHAT et al, 1995), porém utilizando cultivares indianas. Não são conhecidos trabalhos de multiplicação in vitro envolvendo cultivares brasileiras ou adaptadas ao Brasil.…”

Divergence of secondary metabolism in cell suspension cultures and differentiated plants of Piper cernuum and P. crassinervium

“…18 Nevertheless, the establishment of cell suspension cultures and investigation of associated secondary metabolism in Piperaceae species have so far remained untouched. Previous analysis of leaves of P. crassinervium have showed the occurrence of antifungal flavanones, benzoic acids and prenylated hydroquinones.…”