Micropropagation of Brassavola nodosa (L.) Lindl. using SETIS™ bioreactor

Abstract

Brassavola nodosa (L.) Lindl. is a tropical epiphytic orchid showing characteristics of interest for the ornamental nursery industry. However, problems with traditional propagation methods limit the development of a large-scale commercial production system. In addition, this species is considered endangered due to the reduction in population caused by habitat destruction, climate change and overcollection from native areas. The use of micropropagation has been investigated for this species, and the use of liquid in vitro systems showed potential for use of temporary immersion bioreactors for micropropagation of B. nodosa. This study evaluated the e ciency of the SETIS™ bioreactor system for the micropropagation of B. nodosa by adjusting parameters of immersion (frequency and duration) and by comparing it to conventional semi-solid culture systems. Results indicate that temporary immersion of B. nodosa explants with a frequency of 2 h and duration of 2 min returned the highest multiplication rates, with 4.6 shoots produced per explant compared with 2.8 shoots per explant in semi-solid agarbased systems. The use of bioreactors also promoted increased growth and development and in vitro rooting, therefore improving survival and facilitating acclimatization of in vitro-derived plantlets. This is the rst study demonstrating a successful protocol for large-scale micropropagation of B. nodosa using SETIS™ bioreactors, which could have signi cant value and impact for the commercial production of this species as well as for conservation purposes. Key MessageMicropropagation of Brassavola nodosa was achieved using SETIS temporary immersion bioreactors, showing good multiplication rates, increased growth and development and in vitro rooting, thus improving survival and acclimatization ex vitro.

Influence of medium type and culture method on the multiplication efficiency of Chlorophytum comosum (Thunb.) Jacques in in vitro conditions

“…Alister et al [2005] indicated that the use of the temporary immersion bioreactor system RITA® resulted in enhanced shoot elongation in Eucalyptus clones compared to semi-solid media. On the other hand, Vendrame et al [2023] research in Brassavola nodosa (L.) indicated that there were no significant differences in shoot length and leaf number between culture systems, including SETIS™ temporary immersion bioreactors and conventional semi-solid methods, which was consistent with the findings of Kaçar et al [2020].…”

Micropropagation and Acclimatization of Monstera deliciosa Liebm. ‘Thai Constellation’

“…In addition, an increase in chlorophyll synthesis has also been reported by using TIS in the micropropagation of A. andreanum, and the relative chlorophyll content was significantly higher for Brassavola nodosa (L.) Lindl. plantlets produced in TIS bioreactors as compared to those from a semisolid medium [25]. In this experiment, however, in temporary immersion bioreactors, the relative chlorophyll content of the leaves of the explants was significantly lower than that of the control using the traditional method.…”

GreenTray®, a TIS Bioreactor for Plant Micropropagation and Abiotic or Biotic Stress Bioassays

“…The extraction of shoots with forceps from in vitro culture containers, as well as from TIS bioreactors, followed by arranging them on sterile surfaces in order to divide them with the help of surgical scalpels and their subsequent introduction into new culture containers, a process known as subculturing in the conventional in vitro culture process, is time-consuming and represents a significant portion the hand labor involved in most in vitro plant culture processes. The components of the actual GreenTray ® TIS bioreactor described herein, which is a further evolution of the ones presented in the past [31,32], make it significantly improved and versatile compared to other commercial TIS bioreactors, such as RITA ® , Matis ® , Plantform TM , SETIS TM [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17], or the TIS developed by several in vitro plant culture laboratories oriented to solve particular propagation objectives for diverse plant species [18][19][20][21][22][23][24][25][26][27][28].…”

“…Temporary immersion system (TIS) bioreactors have been developed over recent decades to favor plantlet or shoot development. The best-known commercial ones are RITA ® , its bigger version Matis ® [1][2][3][4][5][6][7], Plantform TM [8][9][10][11][12][13][14], and SETIS TM [15][16][17]. In addition, there are several other TISs developed for particular purposes by in vitro plant culture laboratories [18][19][20][21][22][23][24][25][26][27][28].…”