Micropropagation of Brassica oleracea (Cole Crops)

Msikita, W.; Skirvin, R. M.; Chen, S. Y.

doi:10.1007/978-3-662-07774-0_3

Cited by 2 publications

(3 citation statements)

References 49 publications

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“…gemmifera. However, kohlrabi was almost deprived of attention (Msikita et al 1997). Little has changed since successful regeneration from leaf explants by Glendening and Sjolund (1988), and kohlrabi remains the least well studied of all Brassicas.…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium-mediated transformation and gene editing of kohlrabi ( Brassica oleracea var. gongylodes )

Mikhaylova¹,

Musin²,

Panfilova³

et al. 2023

Preprint

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Brassica oleracea var. gongylodes (kohlrabi) is an economically important vegetable with edible stems. Due to the complicated in vitro regeneration, biotechnological cultivars with useful traits do not yet exist. This plant have never been subjected to genetic transformation of any kind. Therefore, the development of appropriate methods is an important task of modern plant science. A genetic construct targeting BoCPC gene have been introduced to Agrobacterium tumefaciens strain Agl0 in order to produce knockout mutants of kohlrabi. Addition of AgNO3 to the culture medium allowed to reduce browning after inoculation. Three glufosinate-resistant, PCR-positive transformants were obtained from cultivar Vienna White. In two of them expression level of target gene was significantly reduced. The described protocol allows for genetic transformation and genome editing of kohlrabi. This research opens up prospects for generation of new cultivars with improved agricultural traits such as stress tolerance, increased yields and altered metabolite content. According to the results of various studies, Vienna White can be used as a model cultivar of kohlrabi.

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Section: Introductionmentioning

confidence: 99%

Agrobacterium-mediated transformation and gene editing of kohlrabi ( Brassica oleracea var. gongylodes )

Mikhaylova¹,

Musin²,

Panfilova³

et al. 2023

Preprint

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“…oleracea is mostly herbaceous (Msikita et al, 1997), suffrutescent perennial species having a short thick vegetative stem (Rakow, 2004). The wild Brassica oleracea, which are considered the progenitor species of cabbage cultivars are restricted to the Coasts of North Spain, Western and Northern France, isle of Helgoland and the British Isles, (Snogerup et al, 1990;Panda et al, 2003).…”

Section: Cabbagementioning

confidence: 99%

“…The domesticated Brassica oleracea cultivars, which are now of cosmopolitan distribution, are commonly called 'cole crops', including cauliflower, broccoli, Brussels sprouts, kohlrabi, kale and cabbage (Haynes et al, 2009), and are likely of the ancient Mediterranean origin (Maggioni et al, 2010). The cultivated B. oleracea are polymorphic, varying in leaf colour, size and shape, and grouped into savoy, red and white forms (Singh et al, 2006) and mostly seasonal (annuals or biennials) plants (Msikita et al, 1997). They are economically important dietary vegetables and one of the most grown cole crops across the world (Maggioni, 2015).…”

Section: Cabbagementioning

confidence: 99%

Physiological and biochemical investigations into the reinvigoration of deteriorated Brassica oleracea L. (Cabbage) and Lactuca sativa L. (Lettuce) seeds with antioxidants and inorganic salt solutions.

Adetunji

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This study focused on reinvigorating deteriorated Brassica oleracea L. (cabbage) and Lactuca sativa L. (lettuce) seeds with antioxidants and inorganic (electrolysed and non-electrolysed) salt solutions. All pre-treatment solutions were applied to fresh (control) and controlled deteriorated (CDd) seeds at 75% viability (P75), 50% viability (P50) and 25% viability (P25). The pre-hydration treatments were compared in terms of their effects on seed germination, seedling vigour, electrolyte conductivity (EC), accumulation of lipid peroxidation products, protein carbonylation (PC), antioxidant enzymes and germination enzymes. The study also investigated the effects of invigorating CDd seeds with selected antioxidants on subsequent seedling growth, gas exchange and photochemistry under greenhouse conditions. Controlled deterioration, in general, resulted in the loss of seed vigour and viability but at higher rates in lettuce than cabbage, and increased EC and PC, and lowered antioxidant and germination enzymes activities in both species. However, significant lipid peroxidation was only recorded in lettuce seeds. Antioxidant pre-treatments enhanced viability of CDd seeds of cabbage at P25 and lettuce at P50 and P25, and improved seedling vigour of fresh, P75 and P50 cabbage, and P50 and P25 lettuce seeds. Antioxidant pretreatments reduced EC and lipid peroxidation in lettuce seeds, while PC was reduced in both species. The treatments also elevated antioxidant and germination enzymes activities in P25 cabbage seeds and P50 and P25 lettuce seeds. The inorganic salt pre-treatments did not enhance percentage seedling production in CDd cabbage seeds. However, in CDd lettuce seeds, Cacontaining solutions and electolysed (cathodic water) treatments promoted percentage normal seedling production and enhanced seedling vigour irrespective of pre-treatment solution pH or seed deterioration level. In the greenhouse studies, certain antioxidants promoted seedling vigour and leaf area in both species and enhanced shoot dry weight and gas exchange in lettuce. In summary, CDd lettuce seeds responded better to both types of invigoration (i.e., antioxidants and inorganic salt solutions) than cabbage seeds, while antioxidants appeared to be relatively more beneficial than inorganic salts in both species. The results suggest that the benefits of certain pre-treatment solutions were based on the enhancement of the activities of key antioxidant and germination enzymes, and the efficiency of photosynthesis at the early stages of growth. The results argue for the use of these seed pre-hydration treatments for mitigating poor stand establishment brought about by seed ageing, and as a useful approach to reinvigorating seeds in long-term storage collections for ensuring global food security.

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Micropropagation of Brassica oleracea (Cole Crops)

Cited by 2 publications

References 49 publications

Agrobacterium-mediated transformation and gene editing of kohlrabi ( Brassica oleracea var. gongylodes )

Agrobacterium-mediated transformation and gene editing of kohlrabi ( Brassica oleracea var. gongylodes )

Physiological and biochemical investigations into the reinvigoration of deteriorated Brassica oleracea L. (Cabbage) and Lactuca sativa L. (Lettuce) seeds with antioxidants and inorganic salt solutions.

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