Micropropagation of bromeliad Aechmea fasciata via floral organ segments and effects of acclimatization on plantlet growth

Huang, Ping-Lung; Liao, Li‐Jen; Tsai, C.-J.; Liu, Zin-Huang

doi:10.1007/s11240-010-9843-0

Cited by 20 publications

(15 citation statements)

References 21 publications

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“…Similarly maximum number of shoots was achieved from leaf section callus of Hyptis suaveolens [34] on MS medium containing 0.5 mg/L BA and 0.5 mg/L GA 3 . In the present investigation, 71.6% of the organogenic calli derived through leaf section explants on MS medium supplemented with 1.0 mg/L TDZ and 0.25 mg/L NAA produced an average of 2.1 shoots per callus explant which was consistent with the previous reports such as, Saussurea obvallata [14] [39], Aechmea fasciata [40] and Echinacea purpurea [26]. Ghimire et al [41] obtained highest mean number (10.65) of shoots per explant from in vitro leaves of Drymaria cordata on medium containing 1.0 mg/L BA and 0.1 mg/L NAA.…”

Section: Discussionsupporting

confidence: 92%

In Vitro Organogenesis of Quisqualis indica Linn.

Mandal¹,

Laxminarayana²

2012

AJPS

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Shoot organogenesis and plant regeneration were achieved on callus derived from leaf section and stem base explants of Quisqualis indica (Combretaceae). In vitro cultures were established using nodal segments obtained from mature field-grown shrubby plants. For the development of optimized protocol, different types and concentrations of plant growth regulators were used to induce adventitious shoot regeneration via callus from leaf section and one-node stem base explants obtained from in vitro regenerated micro shoots and direct field-grown newly flush-off shoots. The TDZ was considered to be the best among the cytokinins (6-benzyladenine (BA), 6-(-, dimethylallyamino purine) (2-iP) and thidiazuron (TDZ) added to the Murashige and Skoog's medium (MS) for adventitious shoot productions. A combination of 1.0 mg/L TDZ and 0.5 mg/L GA 3 was most effective in stimulating callus induction and adventitious shoot regeneration from the leaf section derived calli with an average of 6 shoots per callus explant and an average of 8 shoots per callus explant originated from one-node stem base explants. In vitro raised shoots were sub-cultured on MS medium supplemented with 1.0 mg/L BA and 0.5 mg/L GA 3 for further shoot growth. Maximum rooting of in vitro regenerated shoots was obtained on MS medium supplemented with either 0.5 mg/L indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) individually or a combination of 0.5 mg/L IAA and 0.5 mg/L IBA. Plantlets raised in vitro were acclimatized and subsequently transferred to experimental field.

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Section: Discussionsupporting

confidence: 92%

In Vitro Organogenesis of Quisqualis indica Linn.

Mandal¹,

Laxminarayana²

2012

AJPS

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“…Abiotic factors, such as light (spectral quality or amount), also help to explain the root emission in non-treated explants, in the acclimatization process, for instance, the irradiance levels interfere in the accumulation of photoassimilates (Huang et al 2011b). Some irradiance levels can increase the temperature, what can cause accumulation of metabolites, such as myo-inositol, glucose, fructose and sucrose, and may be related to the plant homeostasis maintenance, mostly in the initial phases of acclimatization (Mollo et al 2011).…”

Section: Resultsmentioning

confidence: 99%

“…Thereby, these authors showed that tissue culture techniques are potentially important to the propagation of this taxonomic group. For the Vriesea reitzii Leme & Costa (Alves et al 2006) and Aechmea fasciata Baker (Huang et al 2011b) species, in vitro and ex vitro rhizogenesis capacity were observed from in vitro shoots.…”

Section: Key-wordsmentioning

confidence: 99%

Effect of synthetic auxins on in vitro and ex vitro bromeliad rooting

Martins

Schimildt

Alexandre

et al. 2013

Pesqui. Agropecu. Trop.

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The tissue culture can contribute to the propagation of several economic species, such as the bromeliads. This research aimed at evaluating the auxins type and concentration in the in vitro and ex vitro rhizogenesis of Neoregelia concentrica bromeliad. N. concentrica shoots were induced in a growth medium with 15.0 µM of 6-benzylaminopurine, for 80 days, followed by sub-cultivation in phytoregulator-free medium, for 45 days. In the in vitro rhizogenesis, the shoots grew in a medium supplemented with indole-3-butyric acid (IBA) or naphthalene-acetic acid (NAA), at the concentrations of 0.0 µM, 1.0 µM, 2.0 µM, 3.0 µM and 4.0 µM. In the ex vitro rhizogenesis, the bases of shoots were immersed, for 60 minutes, in IBA or NAA solutions, at the concentrations of 0.0 µM, 5.0 µM, 10.0 µM and 15.0 µM. After immersion, the shoots were planted in plastic trays with vermiculite. At the end of each rhizogenesis method, the phytotechnical parameters analysis was carried out. For the in vitro rhizogenesis, a higher number of roots were observed when the shoots were cultivated in concentrations higher than 1.0 µM of NAA, when compared to the IBA. However, the rooting rate differed only at 30 days after the in vitro growth, with a higher root induction in the shoots grown with NAA. At 60 days, the rooting rate was higher than 90% and statistically similar in all treatments. In the ex vitro rhizogenesis, a better formation of the rooting system was observed when 5.0 µM of IBA was applied, with higher rooting averages and number of roots.

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“…Also, pistils in rice can be induced from callus, whereas, flowers in Hyacinth were regenerated directly from explants (Lu and Zhu 1991). Shoots of bromeliad were regenerated from callus on petal explants (Huang et al 2011). Choice of cytokinin can influence regeneration type.…”

Section: Discussionmentioning

confidence: 99%

Efficiency of shoot regeneration from leaf, stem, petiole and petal explants of six cultivars of Chrysanthemum morifolium

Song

Mattson

Jeong

2011

Plant Cell Tiss Organ Cult

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Leaf, petal, petiole, and intermodal stems of six chrysanthemum cultivars were incubated on a medium supplemented with 0.00-6.66 lM benzyladenine (BA) either alone or in combination with 2.85-8.56 lM indoleacetic acid (IAA), and 0.46 lM kinetin (Kin). Significant differences in frequency of regeneration were observed among different cultivars when grown on media containing different plant growth regulators (PGRs). The highest frequency of regeneration was observed for cv. Brighton followed by cvs. Dark Roanoke and Tahoe. Low frequencies of shoot organogenesis were observed for cvs. Dark Orange Cupertino, Hudson Bay, and Ivory Eugene. Among different explants, petals exhibited the highest frequencies of shoot organoenesis and mean number of shoots per explant. Regenerated shoots of cv. Brighton transferred onto a medium containing 2.85-8.56 lM IAA developed highest frequency of roots (100%) with a mean of 7.6 roots per shoot. Well-rooted shoots transferred to plastic pots containing a soil mix were kept in a growth room for 1 week, and then moved to the greenhouse for further growth and development.

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Micropropagation of bromeliad Aechmea fasciata via floral organ segments and effects of acclimatization on plantlet growth

Cited by 20 publications

References 21 publications

<i>In Vitro</i> Organogenesis of <i>Quisqualis indica </i>Linn.

<i>In Vitro</i> Organogenesis of <i>Quisqualis indica </i>Linn.

Effect of synthetic auxins on in vitro and ex vitro bromeliad rooting

Efficiency of shoot regeneration from leaf, stem, petiole and petal explants of six cultivars of Chrysanthemum morifolium

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Micropropagation of bromeliad Aechmea fasciata via floral organ segments and effects of acclimatization on plantlet growth

Cited by 20 publications

References 21 publications

&lt;i&gt;In Vitro&lt;/i&gt; Organogenesis of &lt;i&gt;Quisqualis indica &lt;/i&gt;Linn.

&lt;i&gt;In Vitro&lt;/i&gt; Organogenesis of &lt;i&gt;Quisqualis indica &lt;/i&gt;Linn.

Effect of synthetic auxins on in vitro and ex vitro bromeliad rooting

Efficiency of shoot regeneration from leaf, stem, petiole and petal explants of six cultivars of Chrysanthemum morifolium

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<i>In Vitro</i> Organogenesis of <i>Quisqualis indica </i>Linn.

<i>In Vitro</i> Organogenesis of <i>Quisqualis indica </i>Linn.