Micropropagation of Cacti—a Review

Lema-Rumińska, Justyna; Kulus, Dariusz

doi:10.2985/026.019.0107

Cited by 49 publications

(44 citation statements)

References 87 publications

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“…There are also no quantitative studies that examine the relation between the age of the explant tissue, or the age of the mother plant, and the level of contamination. Separately, the correlation between the age of the mother plant and regeneration efficiency has been reported in other species in which juvenile tissues generally resulted in better regeneration than tissues from adult mother plants or were less recalcitrant to regeneration (Liu and Pijut 2008, Cardoso and Habermann 2014, Lema-Rumińska and Kulus 2014. In addition, we observed that in most Dendrobium micropropagation studies that employed somatic tissues as explants, young shoots (0.5 to 12 cm long) were the main explant used rather than mature shoots with leaves or pseudobulbs (Tab.…”

Section: In Vivo Conditions Of Donor Plants and Explant Choicementioning

confidence: 65%

Tissue disinfection for preparation of Dendrobium in vitro culture

Silva¹,

Winarto²,

Dobránszki

et al. 2016

Folia Horticulturae

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Establishing an aseptic in vitro culture for Dendrobium, or for any plant in fact, is the most important step towards developing an effective in vitro tissue culture including micropropagation protocol. Success in initial aseptic culture will contribute to the successful production of in vitro cultures that may involve the initiation or formation of callus and/or protocorm-like bodies (PLBs), the induction, regeneration or multiplication of shoots, and the preparation and proliferation of plantlets suitable for acclimatization. The initiation of an aseptic culture is closely related to the appropriate selection of an explant source and its preparation, including its (in vivo) pre-treatment if necessary and subsequent disinfection procedures. Care in the choice of explant and the application of an appropriate disinfection protocol can successfully reduce, or eliminate, contamination in in vitro cultures while reducing the negative impact on plant tissues and plantlet regeneration. Many unique aseptic culture procedures for Dendrobium genus have been reported in the literature, very often specific to particular tissues or genotypes, and this review not only highlights the details of such protocols, but also provides practical advice for novice -and even seasoned -orchidologists who wish to research Dendrobium in vitro, although it is cautioned that there is currently no universal aseptic culture procedure that can be applied to all conditions, all explants or all genotypes.

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Section: In Vivo Conditions Of Donor Plants and Explant Choicementioning

confidence: 65%

Tissue disinfection for preparation of Dendrobium in vitro culture

Silva¹,

Winarto²,

Dobránszki

et al. 2016

Folia Horticulturae

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“…Due to the contamination issue frequently faced during the establishment of in vitro cultures of cacti species, many investigators have started from in vitro aseptic seedlings or other secondary explants axcissed from in vitro cultures. In general, seeds represent an excellent material because they can be subjected to high concentrations of commercial bleach solution to eliminate microbial contamination, and, thereby, aseptic seedlings may be obtained (Lema-Rumińska and Kulus, 2014).…”

Section: In Vitro Culture Establishmentmentioning

confidence: 99%

“…In general, artificial growth media provide good conditions for either in vitro seed germination of almost all cactus species or for their micropropagation under aseptic conditions and controlled temperature, light and nutrition regimes (Shedbalkar and Adki, 2010). In some cases, full-strength MS (Murashige and Skoog, 1962) medium can inhibit or delay seed germination of cactus species (Lema-Rumińska and Kulus, 2012;Lema-Rumińska and Kulus, 2014).…”

Section: In Vitro Propagationmentioning

confidence: 99%

Tissue culture of ornamental cacti

Pérez-Molphe-Balch

Santos-Díaz

Ramírez-Malagón

et al. 2015

Sci. agric. (Piracicaba, Braz.)

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Cacti species are plants that are well adapted to growing in arid and semiarid regions where the main problem is water availability. Cacti have developed a series of adaptations to cope with water scarcity, such as reduced leaf surface via morphological modifications including spines, cereous cuticles, extended root systems and stem tissue modifications to increase water storage, and crassulacean acid metabolism to reduce transpiration and water loss. Furthermore, seeds of these plants very often exhibit dormancy, a phenomenon that helps to prevent germination when the availability of water is reduced. In general, cactus species exhibit a low growth rate that makes their rapid propagation difficult. Cacti are much appreciated as ornamental plants due to their great variety and diversity of forms and their beautiful short-life flowers; however, due to difficulties in propagating them rapidly to meet market demand, they are very often over-collected in their natural habitats, which leads to numerous species being threatened, endangered or becoming extinct. Therefore, plant tissue culture techniques may facilitate their propagation over a shorter time period than conventional techniques used for commercial purposes; or may help to recover populations of endangered or threatened species for their re-introduction in the wild; or may also be of value to the preservation and conservation of the genetic resources of this important family. Herein we present the state-of-the-art of tissue culture techniques used for ornamental cacti and selected suggestions for solving a number of the problems faced by members of the Cactaceae family.

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“…Cacti-based chemotherapy is a potential alternative to current cancer treatment. After applying in vitro bioreactor systems, valuable cacti metabolites could be produced for a large scale at limited costs and time (Lema-Rumińska and Kulus, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…In the search for alternatives to treat diseases, researchers worldwide have taken the task of finding (Shetty et al, 2012;Harlev et al, 2013;Lema-Rumińska and Kulus, 2014). Pachycereus marginatus (DC.)…”

Section: Introductionmentioning

confidence: 99%

Antitumor activity of Pachycereus marginatus (DC.) Britton Rose extracts against murine lymphoma L5178Y-R and skin melanoma B16F10 cells

Hernandez-Martinez¹,

Gómez-Flores²,

Tamez‐Guerra³

et al. 2016

J. Med. Plants Res.

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Pachycereus marginatus (DC.) Britton & Rose is a species belonging to the family Cactaceae. In traditional medicine, it is recommended to treat diabetes and gastrointestinal infections; however, there are no studies related to its use in cancer treatment. The in vitro antitumor effect of P. marginatus hexane, chloroform, methanol, and methanol-aqueous partition stem extracts, against murine lymphoma L5178Y-R and skin melanoma B16F10 cells, was evaluated in liquid medium by the colorimetric 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) reduction assay. The extracts resulted in up to 84, 85, 84, and 82% cytotoxicity (p < 0.05) to L5178Y-R cells, respectively, and up to 39, 51, 48, and 42% cytotoxicity (p < 0.05) to B16F10 cells, respectively. Vehicle controls were not cytotoxic for tumor cells, and along with the extracts they did not affect viability of resident murine thymus and spleen lymphocytes. Taken together, the present results showed that P. marginatus extracts possess antitumor potential against L5178Y-R lymphoma and B16F10 skin melanoma cells.

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Micropropagation of Cacti—a Review

Cited by 49 publications

References 87 publications

Tissue disinfection for preparation of Dendrobium in vitro culture

Tissue disinfection for preparation of Dendrobium in vitro culture

Tissue culture of ornamental cacti

Antitumor activity of Pachycereus marginatus (DC.) Britton Rose extracts against murine lymphoma L5178Y-R and skin melanoma B16F10 cells

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