Micropropagation of Chestnut (Castanea sativa Mill.) Affected by Plant Growth Regulators under In Vitro Conditions

Tafazoli, Mehrcedeh; Nasr, Seyed Mohammad Hosseini; Jalilvand, Hamid; Tafazoli, Mahya

doi:10.52547/ifej.9.17.114

Cited by 3 publications

(2 citation statements)

References 22 publications

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“…Traditional methods are ineffective for large-scale reproduction of this plant for programs for the rapid renewal of natural or production sites or breeding in order to create disease-resistant germplasm (Liu et al, 2022;Pavese, et al, 2022;Troch et al, 2010). In vitro reproduction is a highly effective tool for rapid and largescale cloning of healthy germplasm from limited plant material under controlled environmental conditions, as well as for long-term maintenance of germplasm (Mullins, 1987;Tafazoli et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Propagation of Azerbaijani chestnut by tissue culture

Balazade,

Hafizov

2024

AFRJBS

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Castanea sativa Mill., an indigenous species of the mountainous Gabala region of Azerbaijan, where its local variety is now facing the danger of extinction. The preservation of this variety of European chestnut requires the development of effective strategies for reliable in vitro regeneration systems as an alternative to traditional methods, which has become the main objective of this study. In solving this problem, the generally accepted technique of micro-multiplication of axillary shoots was mainly used. First, a phased sterilization was carried out using liquid soap, Previkur fungicide and mercury (II) chloride. DKW (Driver and Kuniyuki Walnut) nutrient medium was used for germination of explants, into which growth stimulants BAP (Benzilamunapurine), IBA (Indole -3 butyric acid), IAA (Indole Acetic acid), NAA (Naphthalene Acetic Acid) and GA3 (Gibberelic acid) were introduced in various combinations and quantities. The test of the abovementioned sterilization model revealed significant shortcomings in terms of the acceptability of the results obtained (16-77%). It was also found that the germination of explants takes 14 days and it is better to conduct it in a DKW environment containing hormones BAP (0.6 mg), IBA (0.1 mg) and GA3 (0.1 mg)/1 L DKW. A mixture of BAP (0.1 mg) + IBA (0.35 mg) is more suitable for the reproduction of grown explants + GA3 (0.2 mg)/1 l DKW (the result is 3 new micro-plants for each explant), and for good root formation (it takes 30 days) -a mixture of IBA 1.0 mg + NAA 0.5 mg + IAA 0.5 mg/l L DKW. After the shoots have acquired a certain length (at least 1.5 cm), it is required to transfer them for 22 days to a DKW medium containing IBA (1 mg), IAA (0.5 mg) and NAA(0.5 mg)/l L DKW so that the root splitting process begins and ends.

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Section: Introductionmentioning

confidence: 99%

Propagation of Azerbaijani chestnut by tissue culture

Balazade,

Hafizov

2024

AFRJBS

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“…Traditional methods are ineffective for large-scale reproduction of this plant for programs for the rapid renewal of natural or production sites or breeding in order to create disease-resistant germplasm [3,4,5]. In vitro reproduction is a highly effective tool for rapid and large-scale cloning of healthy germplasm from limited plant material under controlled environmental conditions, as well as for long-term maintenance of germplasm [6,7].…”

Section: Introductionmentioning

confidence: 99%

Propagatıon of Azerbaıjanı Chestnut by Tıssue Culture

Hafizov,

Balazade

2023

Preprint

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Castanea sativa Mill., an indigenous species of the mountainous Gabala region of Azerbaijan, where its local variety is now facing the danger of extinction. The preservation of this variety of European chestnut requires the development of effective strategies for reliable in vitro regeneration systems as an alternative to traditional methods, which has become the main objective of this study. In solving this problem, the generally accepted technique of micro-multiplication of axillary shoots was mainly used. First, a phased sterilization of was carried out using liquid soap, Previkur fungicide and mercury (II) chloride. DKW (Driver and Kuniyuki Walnut) nutrient medium was used for germination of explants, into which growth stimulants BAP (Benzilamunapurine), IBA (Indole - 3 butyric acid), IAA (Indole Acetic acid), NAA (Naphthalene Acetic Acid) and GA3 (Gibberelic acid) were introduced in various combinations and quantities. The test of the above-mentioned sterilization model revealed significant shortcomings in terms of the acceptability of the results obtained (16-77%). It was also found that the germination of explants takes 14 days and it is better to conduct it in a DKW environment containing hormones BAP (0.6 mg), IBA (0.1 mg) and GA3 (0.1 mg) / 1 L DKW. A mixture of BAP (0.1mg) + IBA (0.35 mg) is more suitable for the reproduction of grown explants + GA3 (0.2 mg) / 1 l DKW (the result is 3 new micro-plants for each explant), and for good root formation (it takes 30 days) – a mixture of IBA 1.0 mg + NAA 0.5 mg + IAA 0.5 mg / l L DKW. After the shoots have acquired a certain length (at least 1.5 cm), it is required to transfer them for 22 days to a DKW medium containing IBA (1 mg), IAA (0.5 mg) and NAA(0.5 mg) / l L DKW so that the root splitting process begins and ends.

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