Micropropagation of Cymbidium Sleeping Nymph through protocorm-like bodies production by thin cell layer culture

Vyas, Shivani; Guha, Satyakam; Kapoor, Priyanka; Rao, I. Usha

doi:10.1016/j.scienta.2009.11.020

Cited by 27 publications

(13 citation statements)

References 13 publications

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“…Increase in concentration to 5% of CW promotes elongated shoots (0.72 cm) compared to BP (Fig.1E). Likewise, enhanced shoot development using CW has been in many Dendrobium [19], [41], [43], [21], [48], [31] and in Cymbidium pendulum [15].…”

Section: Resultsmentioning

confidence: 99%

In vitro regeneration from protocorms in Dendrobium aqueum Lindley – An imperiled orchid

Parthibhan

Rao

Kumar

2015

Journal of Genetic Engineering and Biotechnology

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An efficient in vitro plant regeneration protocol from protocorms of Dendrobium aqueum was developed. The uniformly developed protocorms (in vitro origin) having shoot initials were cultured on half macro strength MS medium (1/2 MS) supplemented with cytokinins (BA, 2iP, KIN and TDZ) at 1, 3, 5, 7, 10 mg l À1 , natural additives (BP and CW) at 1%, 3%, 5%, 7%, 10% and auxins (IBA, NAA, 2,4-D) at 1, 3, 5, 7, 10 mg l À1 to study their efficacy on complete plant development. A maximum of 9.4 shoots per explant were generated on 3 mg l À1 of NAA followed by 3% of BP (7.0 shoots). Shoot elongation (1.52 cm) was achieved on 1/2 MS medium fortified with NAA 7 mg l À1 followed by TDZ 7 mg l À1 (1.37 cm). Shoots cultured on 1/2 MS medium supplemented with IBA 5 mg l À1 produced an average of 8.75 roots per shoot, however the lengthiest roots (1.48 cm) were noted in NAA 7 mg l À1 . Healthy rooted plantlets successfully acclimatized in ex vitro condition. The role of complete plantlet production by natural additives could be useful for conservation and cost effective commercial production of orchids.ª 2015 Production and hosting by Elsevier B.V. on behalf

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Section: Resultsmentioning

confidence: 99%

In vitro regeneration from protocorms in Dendrobium aqueum Lindley – An imperiled orchid

Parthibhan

Rao

Kumar

2015

Journal of Genetic Engineering and Biotechnology

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“…Control and FA treated PLBs were selected for histology analysis. The histological procedure applied in this treatment was modified from Vyas et al (2010). Imaging, photography and observations of the slides were carried out with the aid of a light microscope [Olympus BX50, Olympus Optical Co. Ltd., Japan] fitted with a JVC K-F55B colour video camera [JVC Victor Company of Japan, Limited, Japan].…”

Section: Histological Analysis Of Plbsmentioning

confidence: 99%

Preliminary study on the effects of fusaric acid treated protocorm-like bodies of Dendrobium hybrid against Fusarium proliferatum and Fusarium oxysporum

Sangu¹,

Nor²,

Zakaria³

et al. 2019

MJM

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Aims: Dendrobiums are majorly affected by Fusarium proliferatum and F. oxysporum. The aim of this research was to utilise the mycotoxin, fusaric acid (FA) on Dendrobium hybrid to produce cultivars that are resistant towards these fungi. Methodology and results: FA of concentrations 0.05, 0.10, 0.15 and 0.20 mM were transferred to sterilised halfstrength Murashige and Skoog (MS) medium and inoculated with four weeks old thin cell layer (TCL) of protocorm-like bodies (PLBs) for eight weeks. It was deduced that PLBs treated with 0.10 mM of FA resulted in highest survival and shoot regeneration rate but the survival and regeneration rate began to decline as the concentrations of FA were increased. Histology and scanning electron microscopy (SEM) observation showed prominent cell damage and stomatal closure in PLBs treated with FA. Direct amplification of minisatellite DNA (DAMD) markers showed polymorphism in the FA treated PLBs compared to the control PLBs. In the leaf bridge bioassay, plantlets treated with 0.05 mM of FA showed most resistance towards both fungal species. Conclusion, significance and impact of study: Therefore, this research is a preliminary screening study where the optimum concentration of FA was selected based on the reaction of treated TCL of PLBs towards these mutagens.

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“…Control samples and PLBs that survived gamma ray irradiation were selected for histology analysis. The histological procedure applied in this treatment was modified from Vyas et al (2010). Imaging, photography and observations of the slides were carried out with the aid of a light microscope [Olympus BX50, Olympus Optical Co. Ltd., Japan] fitted with a JVC K-F55B colour video camera [JVC Victor Company of Japan, Limited, Japan].…”

Section: Histological Analysis Of Plbsmentioning

confidence: 99%

Study on the Effects of Gamma Irradiation on Protocorm-Like Bodies of Dendrobium Hybrid against Fusarium proliferatum and Fusarium oxysporum

Sangu¹,

Nor²,

Zakaria³

et al. 2019

TLSR

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Micropropagation of Cymbidium Sleeping Nymph through protocorm-like bodies production by thin cell layer culture

Cited by 27 publications

References 13 publications

In vitro regeneration from protocorms in Dendrobium aqueum Lindley – An imperiled orchid

In vitro regeneration from protocorms in Dendrobium aqueum Lindley – An imperiled orchid

Preliminary study on the effects of fusaric acid treated protocorm-like bodies of Dendrobium hybrid against Fusarium proliferatum and Fusarium oxysporum

Study on the Effects of Gamma Irradiation on Protocorm-Like Bodies of Dendrobium Hybrid against Fusarium proliferatum and Fusarium oxysporum

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