Micropropagation of dahlia in static liquid medium using slow-release tools of medium ingredients

An efficient method for in vitro propagation of Alstroemeria pallida Graham rhizomes

“…Previous results have shown that supplementation with agar and Gelrite at 5 and 1.5 g L -1 , respectively, showed the optimum results in terms of in vitro growth and development of both roots and shoots of Maranta leuconeura (Ebrahim and Ibrahim, 2000). Specifically for alstroemeria, liquid medium using a slow release of medium components has shown good results (de Klerk and ter Brugge, 2010), although most of the in vitro cultures of alstroemeria rhizomes have been conducted using gelling agents such as agar at concentrations between 7.0 and 8.0 g L -1 (Seyyedyousefi et al, 2013;Hutchinson et al, 2014) and Gelrite at concentrations between 0.2% and 0.3% (m/v) (Hamidoghli et al, 2007;Pumisutapon et al, 2012).…”

“…In addition to the type of explant, characteristics of the culture medium are crucial to achieving successful micropropagation. In particular, the effect of the concentration of gelling agents (Ebrahim and Ibrahim, 2000;de Klerk and ter Brugge, 2010) and plant growth regulators (Cruz et al, 2003;Hamidoghli et al, 2007) have been widely studied in the micropropagation of alstroemeria and other species. Agar is the most common gelling agent and can influence the type of growth response of micropropagated plants.…”

In vitro production of plant material from fructans-producing species

“…It has been reported that the effect of gibberellic acid on the in vitro multiplication of Dahlia spp. and the concentration of 1mg.l-1 in the culture medium, caused an increase in the height of the plant, as well as the number of segments/explant, 12 also De Klerk & Brugge., 13 reported that growth of dahlia (Dahlia hybrida) shoots in vitro was ca. 4times faster in liquid medium than on solidified medium, in liquid standard medium (3% sucrose, macroelements according to Driver-Kuniyuki Walnut medium, microelements according to Murashige-Skoog medium, 0.44M benzylaminopurine).…”

High Efficiency of Propagation for Sagittaria sagittifolia Using a Temporary Immersion Bioreactor System

“…The reason for these results may due to TIBS with temporary immersions uptake of medium ingredients and plant growth regulators over the whole plant surface, especially leaves, can improve the growth of plantlets [23,24]; and medium components were transferred to growing regions with shorter distance. High cytokinin concentrations can promote vitrification [25].…”