Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study

Gianguzzi, Valeria; Barone, Giulio; Gristina, Emilio Di; Sottile, Francesco; Domina, Gianniantonio

doi:10.3390/plants12071530

Cited by 6 publications

(5 citation statements)

References 63 publications

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“…On the other hand, asexual propagation methods like cuttings have not been adequately effective [13], while vegetative propagation by grafting onto quince is seldom used due to compatibility issues [14], with respect to studies on in vitro approaches [15][16][17][18][19][20][21][22]. In vitro propagation is a useful tool for both propagation and safeguarding genotypes with high genetic erosion, using methods that enhance the efficiency of materials and significantly reduce environmental impact [23][24][25]. The efficiency of the regeneration of plant shoots is affected by the type of explant, composition of the medium, and mixture of phytohormones and growth additives [26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Temporary Immersion System as an Innovative Approach for In Vitro Propagation of Sorbus domestica L.

Gianguzzi,

Sottile

2024

Horticulturae

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The genus Sorbus has maintained an extremely relevant role over time from a landscape and environmental perspective in many countries in the Mediterranean and Central Europe. Based on the requirements coming from the environmental policies provided in the European strategy Next Generation EU, Sorbus has been considered a valuable species to be introduced in urban and peri-urban areas. The purpose of this study was to propagate four Sorbus accessions selected in the Sicilian territory, Southern Italy, using the liquid substrate in temporary immersion bioreactors Plantform™. The results obtained showed that the presence of 1 mg L−1 mT in the substrate in combination with IBA 0.05 mg L−1 produced a significant number of shoots (4.7) and a greater length (2.2 cm). Among the accessions, there were statistically significant differences; the accession SN2 and SN1 produced more shoots (respectively, 4.0 and 3.6), and a greater length of the shoots was observed in the selections SN4 and SN3 (respectively, 2.4 cm and 2.3 cm). The relative growth rate (RGR) was significantly influenced by the presence of the culture substrate of the combination of cytokines and auxin; SN4 selection showed the best RGR results of 8.3 mg−1 d−1. The use of the bioreactor Plantform™ in Sorbus domestica L. has favored a better development of plants obtained in vitro, demonstrating that this system is a valid alternative for the micropropagation of Sorbus.

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Section: Introductionmentioning

confidence: 99%

Temporary Immersion System as an Innovative Approach for In Vitro Propagation of Sorbus domestica L.

Gianguzzi,

Sottile

2024

Horticulturae

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“…macrocephala (Huter, Porta & Rigo) Dillenb. & Kadereit ( Gianguzzi et al, 2023 ), Carlina acaulis L. ( Trejgell, Dąbrowska & Tretyn, 2009 ), Echinacea purpurea (L.) Moench ( Korach et al, 2002 ) and Eclipta alba (L.) Hassk. ( Dhaka & Kothari, 2005 ), benefit from the combined effect of cytokinins and auxins, and the highest multiplication rate was achieved with supplementation with BA MS medium.…”

Section: Discussionmentioning

confidence: 99%

“…The efficacy of IBA for rooting in regenerating shoots has been reported for several species, such as Adenostyles alpina subsp. macrocephala ( Gianguzzi et al, 2023 ) and Capparis spinosa L. ( Gianguzzi, Barone & Sottile, 2020 ). The acclimation step was successfully performed with Jiffy-7® pellets.…”

Section: Discussionmentioning

confidence: 99%

“…Micropropagation and other in vitro techniques have been widely used in conservation for the propagation of endangered species and species that present difficulties with traditional propagation techniques. Many threatened species can be quickly propagated with this technology using a minimum of plant material and reducing the impact on populations ( Bengi & Yeald, 2005 ; Trejgell, Michalska & Tretyn, 2010 ; Gianguzzi et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

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Seed germination and vegetative and in vitro propagation of Hieracium lucidum subsp. lucidum (Asteraceae), a critically endangered endemic taxon of the Sicilian flora

Gianguzzi,

Di Gristina,

Barone

et al. 2024

PeerJ

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Hieracium lucidum subsp. lucidum is a critically endangered endemic taxa of the Sicilian flora. It is a relict of the Tertiary period surviving on the cliffs of Monte Gallo (NW-Sicily). This research focused on finding the best protocols for seed germination and vegetative and in vitro propagation to contribute to ex situ conservation. Seed germination tests were carried out using constant temperatures of 15 °C, 20 °C and 25 °C in continuous darkness and an alternating temperature of 30/15 °C (16 h/8 h, light/dark). The seeds had no dormancy, and a high germination capacity (70–95%) was obtained at all tested thermoperiods. The possibility of vegetative propagation of the taxon was evaluated through the rooting capacity of stem cuttings treated or not treated with indole-3-butyric acid (IBA). All cuttings were treated with IBA rooted within 2 months, while only 50% of the untreated cuttings were rooted within a longer time. An efficient protocol for rapid in vitro propagation from leaf portions was developed. The response of explants was tested on hormone-free Murashige and Skoog (MS) basal medium and MS enriched with different types of cytokinins: 6-Benzylaminopurine (BAP) and meta-Topolin (mT) in combination with naphthaleneacetic acid (NAA) and 2,4-Dichlorophenoxyacetic acid (2,4-D) at the same concentration. The combination of mT (2 mg L−1) and 2,4-D (1 mg L−1) in the medium was the most effective and showed the highest percentage of callus induction and the mean number of regenerated shoots. The maximum rate of root regeneration and the maximum number and length of roots were obtained on hormone-free MS and MS enriched with IBA at concentrations of 1 mg L−1. From the results obtained, it can be concluded that H. lucidum subsp. lucidum can be successfully propagated using one of the tested techniques, subject to the availability of the material for reproduction.

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“…Plant tissue culture is an important technique that can serve as a tool in order to enhance propagation over a short period [15][16][17]. Plant tissue culture has been documented as a sustainable way to exploit the most important plant species in propagation and ex situ conservation [14][15][16][17]. This is because it assists the conservation of plant biodiversity [14].…”

Section: Introductionmentioning

confidence: 99%

A Sustainable Approach to In Vitro Propagation and Conservation of Salvia dominica L.: A Wild Medicinal Plant from Jordan

Al-Qudah,

Shibli,

Zatimeh

et al. 2023

Sustainability

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Salvia dominica L. is an important wild medicinal plant that grows in Jordan and neighboring countries, and this plant has been suffering from many threats in its wild environment. Therefore, this research aims to establish a comprehensive and sustainable approach via an in vitro propagation and conservation system for the S. dominica L. plant. Axillary buds were used to initiate the in vitro culture on Murashige and Skoog MS media supplemented with 0.5 mg L−1 of GA3. In vitro shoot proliferation and rooting were experimented on with different concentrations of cytokinins and auxins, respectively. Calli were induced in the dark on excised leaf discs (0.5 cm in diameter), and multiplication was experimented on with different growth regulators. Cryopreservation experiments were applied on the callused segments under different growth conditions via the vitrification technique. A full protocol was achieved for shoot proliferation with 6.3 shoots/explant using 1.2 mg L−1 of thidiazuron (TDZ), while rooting was achieved at 1.5 mg L−1 of NAA with 6.6 functional roots/explant. Acclimatization was completely successful for the rooted plants. The highest callus production with 5.81 g/calli was achieved using 1.5 mg L−1 of benzylaminopurine (BAP). Cryopreservation of the S. dominica calli was successfully achieved when a pure plant vitrification solution (PVS2) was used to dehydrate the calli for 20 min after immersion in the loading solution for 20 min with a 76.6% regrowth percentage. The loading and the plant vitrification solution type and duration were the most critical points in the regrowth of the cryopreserved calli. In conclusion, a successful protocol was set up for the in vitro propagation and conservation of S. dominica calli. This study has prompted us to perform further studies on sustainable in vitro production and conservation of critically endangered medicinal plants to implement a green environment protecting against surrounding threats.

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Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study

Cited by 6 publications

References 63 publications

Temporary Immersion System as an Innovative Approach for In Vitro Propagation of Sorbus domestica L.

Temporary Immersion System as an Innovative Approach for In Vitro Propagation of Sorbus domestica L.

Seed germination and vegetative and in vitro propagation of Hieracium lucidum subsp. lucidum (Asteraceae), a critically endangered endemic taxon of the Sicilian flora

A Sustainable Approach to In Vitro Propagation and Conservation of Salvia dominica L.: A Wild Medicinal Plant from Jordan

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