Micropropagation of GF 677 Rootstock

Abstract

The aim of this investigation was to study micropropagation of Almond GF rootstock on in vitro. Explants were cultured in MS medium containing 5 concentrations of 6-Benzylaminoporine (BAP) 0.1, 0.6, 1, 1.5 and 2 mg 1 -1 along with control treatment. Each treatment includes 5 replications. After 5 weeks of culture, the results showed that the number and length of shoots in treatment containing 1 mg 1 -1 BAP was more than the other treatments and control. The number of shoot that was obtained in treatment of 1 mg 1 -1 BAP was 2.98 (Table 1). The lowest number of shoot was 0.4 due to control treatment. For rooting, explants with 3-5 cm length were transferred to different rooting treatments ( Table 2). The results revealed that the best rooting was obtained with treatment 3 mg 1 -1 IBA. Number and length of roots were 2.167 and 11.53, respectively. Also results showed that putting explants in dark for 1 week is more effective than light.

Effects of nutrient media, different cytokinin types and their concentrations on in vitro multiplication of G×N15 (hybrid of almond×peach) vegetative rootstock

“…These results are conformed with the results reported by Nazary Moghaddam Aghaye et al [32] in GF677 micropropagation, which suggested that BAP is more suitable for shoot multiplication of GF677. Our results showed that callus was significantly affected by medium and cytokinin type so that increasing concentrations of BAP and BA in culture medium brought about a significant enhancement in the callus weight in both experiments.…”

“…Our results are in agreement with those of Unek et al [10] on G · N15 (Garnem) that MS medium containing BAP was the best medium for proliferation of garnem. In contrast to our results, earlier, for G · N9, WPM and QL were found to have a better effect on shoot proliferation rate than either MS or ½ MS medium and the possible explanation given for this was the reduced nitrogen content in WPM [13] Although Nazary Moghaddam Aghaye et al [32] and Molassiotis et al [3] reported that for GF677, use of MS resulted in the best shoot proliferation rates. Dejampour et al [21] found that, the use of DKW resulted in the best proliferation rate.…”

“…Our results showed that callus was significantly affected by medium and cytokinin type so that increasing concentrations of BAP and BA in culture medium brought about a significant enhancement in the callus weight in both experiments. These findings are in agreement with that of Gisela 6 semi-dwarf rootstock [31]. It shows that when concentration of BAP was in excessive amount, it resulted in an increase of callus weight and reduction in micro-shoot length.…”

“…Goudarzi [38], in proliferation of colt and F12.1 rootstocks, showed that BA is the most effective cytokinin. Besides, Daneshvar-Hosseini et al [1] and Nazary Moghaddam Aghaye et al [31] stated that BAP is more effective on proliferation on Gisela 6 rootstock. These findings confirmed our results.…”

“…Micro-propagation has been considered as an efficient tool for mass production of pathogen free and true to type fruit tree rootstocks in recent decades in Iran [31,21]. In order to establish commercial orchards, production of true to type vegetative rootstocks through tissue culture is a matter of high importance.…”