Micropropagation of Hardy Hibiscus (Hibiscus Moscheutos L.)

Abstract

Hardy hibiscus (Hibiscus x moscheutos L.) is gaining popularity as a landscape plant and the accelerated increase in the volume of planting material is of particular relevance. The work investigated the effect of the concentration of 6-BAP (0.5, 1.0, 1.5 mg / L) on the shoot formation of hibiscus of the following varieties: cv. Cranberry Crush, Fantasia, Fireball, Jazzberry Jam. It was revealed that the concentration of 6-BAP 0.5 mg / L is optimal for the proliferation of microshoots of Hibiscus x moscheutos L. With the increase in the concentration of 6-BAP, the number of formed microshoots decreases.

Preliminary study on in vitro shoot culture of Hibiscus coddii subsp. barnardii, an indigenous South African flowering plant

“…barnardii explants occurred with an increase in the BAP concentration to 0.75 and 1 mg L -1 (Table 1). Similarly, nodal shoot explants of H. moscheutos cultured on a lower (0.50 mg L -1 ) BAP concentration formed 4.33 shoots per explant compared to three shoots per explant with a higher (1 mg L -1 ) BAP concentration (Lobodina et al, 2020). In contrast, the best bud sprout response (96%) on nodal explants of H. sabdariffa was reported on MS medium supplemented with 1 mg L -1 BAP (Manokari et al, 2016).…”

“…In contrast, Dar et al (2012) reported 88% survival and no contamination of nodal explants of H. rosa-sinensis collected from an outside garden when surface-disinfected with 50% (v/v) Clorox ® [5.25% (w/v) NaOCl] bleach solution containing a higher concentration (2.63%) of NaOCl. In general, however, microbial contaminations of in vitro cultures are found to occur more readily when the explant source is taken directly from field grown plants (Lobodina et al, 2020). Disinfection of nodal explants is considered to be more difficult since the attachment of the petiole to the stem at an angle creates a V-shaped trough wherein contaminants can be trapped, making it more difficult to sterilize nodal explants with axillary buds (Dar et al, 2012).…”

“…Studies of other Hibiscus species reported the use of BAP for in vitro shoot induction, although the response was dependent on species and explant type (Bhalla et al, 2009;Sami et al, 2016;Lobodina et al, 2020). Based on a pilot study (2, 3 and 4 mg L -BAP), the effect of lower BAP concentrations (0.25, 0.50, 0.75 and 1 mg L -1 ) on in vitro shoot induction of apical and basal seedling shoot explants of H. coddii subsp.…”

“…Shoot culture is the most commonly used micropropagation technique for multiplication and plant regeneration (Davies et al, 2017;Debnath and Arigundam, 2020), and was used in the micropropagation of several Hibiscus species using different explant sources. Mature plants of Hibiscus moscheutos and Hibiscus rosa-sinensis, growing naturally or cultivated in a greenhouse, were used as source of nodal explants for shoot culture after surface disinfection of the plant material (Christensen et al, 2008;Manokari et al, 2016;Lobodina et al, 2020). However, in most instances, in vitro germinated seedlings of Hibiscus species served as aseptic explant source for shoot culture.…”

“…6-Benzyl-aminopurine (BAP) is reported to be the most effective cytokinin for shoot induction, since it can cause proliferation of more axillary buds in a shorter period of time (Bhalla et al, 2009;Bekircan et al, 2018;Faisal et al, 2020) than others. Micropropagation studies of some Hibiscus species, such as Hibiscus sabdariffa, H. moscheutos and H. rosa-sinensis showed that the shoot induction response depended on the BAP concentration, plant species and explant type (Kumar et al, 2016;Faisal et al, 2020;Lobodina et al, 2020). For instance, Ayadi et al (2011) reported a better shoot induction response when Hibiscus cannabinus explants were cultured on media without cytokinins.…”