Micropropagation of Asparagus officinalis L. through callus development

Abstract

In order to develop an in vitro propagation process for production and conservation, in this study, we investigated the influence of disinfectants and plants growth regulators (PGR) on the infection rate of explants, the ability of callus formation, and the regeneration of shoot and root from Asparagus officinalis L. trunk segments. We obtained the best results when disinfecting trunk segments explants combined with alcohol 70° in 5 minutes and NaClO in 5 minutes with the lowest contamination rate of 3.7%. Callus was formed well in MS medium supplemented with 3% sucrose; 0.7% agar, pH= 5.8; 1.0 mg/l BAP and combined with 0.5 mg/l NAA, 1.5 mg/l BAP and 0.75 mg/l NAA. The highest rate of shoot formation was 87.2% when the callus continued to grow in the environment supplemented with 2.0 mg/l BAP and 1.0 mg/l NAA. Roots were formed after 4 weeks of shoot cultivation in MS medium supplemented with 0.5 mg/l NAA with the highest rooting rate of 74.59%.

Effect of Organic Additives on the Micropropagation of Asparagus officinalis

“…It has also been reported that auxins alone can promote root formation in A. officinalis. For instance, Maung et al [15] showed that 2.5 mg/L IBA resulted in the highest rooting rate of 44%, while Minh et al [16] discovered that NAA at a concentration of 0.5 mg/L substantially improved the rooting rate by up to 74.59%. Considering the effect of CW on the in vitro rooting of A. officinalis, the concentrations of 5% and 10% (v/v) CW did not significantly induce root formation in the plant, but instead promoted root elongation, yielding approximately root lengths of 4.54 and 4.88 cm, respectively.…”

“…Another study by Maung et al [15] reported that MS medium supplemented with 2.0 mg/L BAP produced the highest values for the number of shoots (9 shoots/explant), shoot length (2 cm), and number of nodes (2 nodes/shoot), while the highest rooting rate (40%) was observed in medium supplemented with 2.5 mg/L IBA. Most recently, Minh et al [16] demonstrated that MS medium supplemented with 2.0 mg/L BAP and 1.0 mg/L NAA resulted in the highest shoot formation rate (87.2%), while MS medium supplemented with 0.5 mg/L NAA yielded the highest rooting rate (74.59%) of in vitro A. officinalis plantlets.…”