Micropropagation of <i>Dendrobium cruentum</i> Rchb. f., a rare Thai orchid species

Sangdum, S.; Thammasiri, K.; Chuenboonngarm, Ngarmnij; Sopalun, Kathawut

doi:10.17660/actahortic.2017.1167.10

Cited by 3 publications

(3 citation statements)

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“…The dominant characteristics of this orchid are as follows; the length of their pseudobulbs can reach 30 -40 cm; 1 -2 flowers are located on the same apex, and their petals are yellow and green with brick-red color in the lip. The dominant feature of this orchid is that its flowers appear throughout the year [5]. Because of its unique beautiful characteristics, D. cruentum has become the most popular ornamental and commercial orchid in Thailand.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, to date, Thailand is one of the largest producers and exporters of D. cruentum globally [1]. Currently, the numbers of D. cruentum in nature have been greatly decreasing because of illegal overcollection for trade and natural habitat destruction; as a result, the species is at risk of extinction [5]. D. cruentum is listed on Appendix I of the convention on international trade in endangered species of wild fauna and flora as a 'World Endangered Orchid Species".…”

Section: Introductionmentioning

confidence: 99%

“…Based on the abovementioned situation, effective protocols for D. cruentum propagation are crucial and urgently needed. Sangdum et al [5] reported that using the inorganic plant growth regulators, 2 of 11 naphthaleneacetic acid (NAA) and 6-benzyladenine (BA), successfully promoted the micropropagation of D. cruentum in vitro via shoot tips [5]. As inorganic additives, organic additives including coconut water, potato homogenate, banana homogenate, tomato juice, chitosan, peptone, and yeast extract have been widely used in orchid propagation when using tissue culture [6,7].…”

Section: Introductionmentioning

confidence: 99%

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Influences of Organic Additives on Asymbiotic Seed Germination of Dendrobium cruentum Rchb. f. for In Vitro Micropropagation

Samala

Thipwong²

2023

Trends Sci

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Dendrobium cruentum Rchb. f., a commercial orchid in Thailand, faces the risk of extinction and should be conserved, and propagated for commercial. Therefore, the aim of this research was to identify a suitable VW medium supplemented with organic additives for the micropropagation of D. cruentum through asymbiotic seed germination. The VW media supplemented with separate organic additives (coconut water (CW) (100 mL/L), ‘Hom Thong’ banana homogenate (BH) (1000 mg/L), and potato homogenate (PH) (1000 mg/L) or its combination was determined, and the seed germination rate (%), protocorm formation, and plantlet development were observed. The results showed that a combination of BH and PH resulted in the highest seed germination (94.69 ± 1.55 %) when compared to basal VW (control). Protocorm formation was highest with CW alone (47.38 ± 7.17 %) and a combination of CW and PH (52.13 ± 6.20 %). Additionally, after 10 weeks of culture, supplementation with BH yielded the highest number of plantlets. However, after 24 weeks of culture, each organic additives gave the different developments of healthy plantlets. To investigate suitable potting media for acclimatization of well-grown plantlets in greenhouse conditions, 2 pieces of foam, dried coconut husk, and a combination of peat moss: Sphagnum moss: Pumice: Perlite (3:1:1:1) were investigated. The highest survival rate percentage was found in foam-planted plantlets (80 %). Ultimately, the effectively simple mediums containing organic additives and a one-step protocol were provided for the micropropagation of D. cruentum via seeds, and the derived information could be used for D. cruentum conservation and production. HIGHLIGHTS The effects of organic additives on asymbiotic seed germination for in vitro micropropagation of Dendrobium cruentum Rchb. f. was firstly reported The media supplemented with organic additives yield significantly the highest seed germination, protocorm formation, and plantlet formation and development of D. cruentum when compared to basal media In greenhouse conditions, 2 pieces of foam gave the highest survival rate percentage of plantlets (80 %) The practical and beneficial media supplemented with organic additives for micropropagation of D. cruentum was provided GRAPHICAL ABSTRACT

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%