Micropropagation of Jatropha curcas superior genotypes and evaluation of clonal fidelity by target region amplification polymorphism (TRAP) molecular marker and flow cytometry

Franco, Mariana Crotti; Marques, Daniela de Argollo; Siqueira, Walter José; Latado, Rodrigo Rocha

doi:10.5897/ajb2014.13649

Cited by 9 publications

(2 citation statements)

References 38 publications

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“…There are a number of reports in which the use of flow cytometry has enabled the analysis of changes in the stability of the clones; for example, in the species Vitis vinifera, Leal et al (2006) reported the analysis of the ploidy level and Prado et al (2010) detected somaclonal variants; moreover, in J. curcas, Franco et al (2014) analyzed the clonal fidelity among genotypes, Rathore et al (2014) genetic homogeneity and de Oliveira et al (2013) performed the analysis of polyploidization. The aim of the present study was to develop a protocol of in vitro indirect organogenesis for J. curcas variety ALJC01, a high seed yield variety, in order to propagate plant material for future research and agronomic applications.…”

Section: Introductionmentioning

confidence: 99%

INDIRECT ORGANOGENESIS AND ESTIMATION OF NUCLEAR DNA CONTENT IN REGENERATED CLONES OF A NON-TOXIC VARIETY OF Jatropha curcas

Herrera-Cool¹,

João

Rodríguez‐Buenfil³

et al. 2019

Trop Subtrop Agroecosyst

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Jatropha curcas L. is a second-generation energy crop, which produces approximately 40% oil in its seeds, which can be transformed into biodiesel. In vitro culture is a valuable tool for the multiplication and conservation of elite plant varieties. In J. curcas, there are several reports on the micropropagation of the species, but with low reproducibility. The objective of this study was to obtain the in vitro organogenesis of J. curcas and estimate the nuclear DNA content by flow cytometry during eight subcultures in vitro. The organogenesis of adventitious shoots was obtained in 3.3 g/l of MS, 110.25 μM of 6–(γ, γ-Dimethylallylamino) purine (2ip), 1.27 μM of indoleacetic acid (IAA) and 369.21 μM of adenine sulfate (AdS), obtaining up to 18.50 ± 0.7 shoots per explant. The development of the shoots was 1.67 ± 0.76 cm in MS medium, 4.44 μM of benzylaminopurine (BAP), 1.0 μM of IAA and 543 μM of AdS. The rooting was 13.6 ± 2.51 roots in ½ MS and 14.7 μM of indole butyric acid (IBA). The nuclear DNA content was from 0.80 ± 0.12 to 1.07 ± 0.23 pg of nuclear DNA during the eight subcultures.

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Section: Introductionmentioning

confidence: 99%

INDIRECT ORGANOGENESIS AND ESTIMATION OF NUCLEAR DNA CONTENT IN REGENERATED CLONES OF A NON-TOXIC VARIETY OF Jatropha curcas

Herrera-Cool¹,

João

Rodríguez‐Buenfil³

et al. 2019

Trop Subtrop Agroecosyst

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show abstract

“…The most suitable method for large-scale seedling production is in vitro propagation, which is one of the most practical high-impact applications of tissue culture. However, especially for species of the genus Jatropha, this technique presents great challenges to be overcome, such as: determination of the explants most suitable for organogenesis, induction of development and/or elongation of shoots, induction of rooting, among others (Franco et al, 2014).…”

Section: Introductionmentioning

confidence: 99%