Micropropagation of Maclura tinctoria L.: an endangered woody species

Gomes, Guilherme Augusto Canella; Paiva, Renato; Herrera, Raírys Cravo; Paiva, Patrícia Duarte de Oliveira

doi:10.1590/s0100-67622010000100003

Cited by 18 publications

(21 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…x Poncirus trifoliata (L.) Raf., which exhibited a positive correlation with these variables and dosages of activated charcoal (RIBEIRO et al, 2000). Data from this study further supported those obtained by Lédo et al (2007) and Gomes et al (2010) who had better root development for mangabeira plants (Hancornia speciosa) and the explant Maclura tinctoria, respectively, when cultured in vitro. It is evident that activated charcoal is important in the development of vegetative structures because dose and growth are positively correlated.…”

Section: Resultssupporting

confidence: 87%

“…Several authors have concluded that despite the beneficial effect of activated charcoal, including the absorption of toxic components and medium impurities, it also acts on the useful agents, such as sucrose, salts, and in some cases growth regulators, making them inactive or unavailable for the explant. Thus, they suggest that concentrations between 0.1% and 2.0% are within the desirable range for the safe application of this component (BOULAY, 1964;CHAGAS et al, 2005;GRATTAPAGLIA;MACHADO, 1998;RICCI et al, 2002). Means followed by the same capital letter in a column do not differ by Tukey test at 5% probability; CV: coefficient of variation; LSD: least significant difference.…”

Section: Resultsmentioning

confidence: 97%

“…The application of activated charcoal to the culture medium has the ability to adsorb toxic substances, other components, or impurities released by explants (GRATTAPAGLIA; MACHADO, 1998). George and Ravishankar (1997) stated that activated charcoal further attenuates the light interception in the culture medium, stimulating rooting.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In vitro establishment and early development of barueiro (Dipteryx alata Vogel)

Silva

Asmar

Oliveira

et al. 2016

SCA

View full text Add to dashboard Cite

The barueiro (Dipteryx alata Vog.) is a native fruit species of the Cerrado ecoregion that has multiple uses. It is a wild species, and its cultivation is difficult. Furthermore, it is threatened with extinction. Plant tissue culture is a major tool for the conservation of germplasm, as well as a means of propagating highquality seedlings on a large scale. However, this technique has not been used with barueiro, although it might provide valuable contributions to the process of barueiro domestication. The most popular method of cultivation is the use of the Murashige and Skoog medium (MS), which is considered one of the most nutritionally complete media. Woody plant medium (WPM) is indicated for the propagation of woody species, but there are no reports of its use for barueiro cultivation. Woody plants tend to have problems with rust in vitro during the establishment phase. Activated charcoal acts as an adjuvant for the adsorption of phenolic compounds, mitigating its effects in the medium. Thus, the objective of this study was to test four activated charcoal doses (0, 2, 4 and 6 g L -1 ) and three culture media: MS, WPM, and AA (over water agar) in the in vitro establishment of barueiro. The experimental design was a completely randomised (DIC), 4 × 3 factorial design with three replications. At 60 days after inoculation, the explants were evaluated for dry matter, fresh weight, stem diameter, shoot length, number of leaves, longest root length, germination rate, and chlorophyll contents. The MS medium supplemented with 3,0 g L -1 activated charcoal appeared to be the best for in vitro establishment of barueiro. Key words: Native fruit. Activated charcoal. Culture medium. ResumoO barueiro (Dipteryx alata Vog.) é uma espécie frutífera nativa do Cerrado que possui múltiplas possibilidades de utilização. Por estar em estado praticamente selvagem, seu cultivo é dificultado. Além disso, encontra-se ameaçada de extinção. A cultura de tecidos vegetais constitui uma importante ferramenta para a conservação de germoplasma, além de proporcionar a rápida propagação de mudas de qualidade em larga escala. Porém, esta técnica não vem sendo utilizada em barueiro, mas pode ser aplicada de forma valiosa contribuindo para seu processo de domesticação. O meio de cultivo mais utilizado é o MS, considerado um dos mais completos nutricionalmente. O meio WPM é indicado para a propagação de espécies lenhosas, porém não há relatos da sua utilização para o cultivo do barueiro.

show abstract

Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 97%

See 1 more Smart Citation

In vitro establishment and early development of barueiro (Dipteryx alata Vogel)

Silva

Asmar

Oliveira

et al. 2016

SCA

View full text Add to dashboard Cite

show abstract

“…However, the aerial shoots from apical segments treated with IBA had a better response to rooting, nearly doubling the number of rooted shoots. The obtainment of micropropagated plants in two steps was also reported by Gomes et al (2010), in a study on in vitro propagation of Maclura tinctoria (Moraceae), where the maximum shoot formation was obtained when 5.37 µM NAA + 4.45 µM BAP was used and root formation was observed on explants inoculated in WPM and supplemented with 23.62 µM IBA + 4.7 g L -1 activated charcoal. Fermino Junior and Scherwinski-Pereira (2012), using nodal segments from seedlings germinated in vitro in the micropropagation of Amburana acreana (Fabaceae), found that the formation of the largest number of aerial shoots occurred with 4.0 mg L -1 of BAP and the formation of a greater number of roots occurred with 0.5 mg L -1 of IBA.…”

Section: Resultssupporting

confidence: 55%

PROPAGAÇÃO IN VITRO DE <i>Casearia sylvestris</i> SWARTZ (SALICACEAE)

Paranhos¹,

Löbler²,

Cechin³

et al. 2017

Ciênc. Florest.

View full text Add to dashboard Cite

In order to do an in vitro propagation of Casearia sylvestris, a tree native to Brazilian forests, studies of in vitro seed germination were performed and aseptic seedlings obtained were used as explant donors for micropropagation. Mature and immature seeds, stored for 10 days at 10 or 25°C were inoculated in medium containing 10% of MS salts and kept in the light (a 16-hour photoperiod) or continuous dark, at 25°C. For immature seeds, gibberellic acid (GA 3 ) was or was not tested at a concentration of 1.5 mg l -1 . From the seedlings obtained, the cotyledon and apical segments were removed and cultured in complete MS medium with five combinations of 6-benzylaminopurine (BAP) and naphthalene acetic acid (NAA): 0.0; 0.25; 0.5; 1.0; 2.0 and 0.0; 0025; 0.05; 0.1; 0.2 mg L-1, respectively. The aerial shoots formed in vitro were cultured in MS medium containing 0.0 or 1.5 mg L -1 indole-3-butyric acid (IBA) for rooting. The highest percentage of germination (60%) occurred in mature seeds exposed to light, regardless of other treatments, and may be considered positive photoblastic. The highest percentage of germination of immature seeds (27%) occurred when previously stored at 10 ° C with or without GA 3 . The induction of aerial shoots was more efficient in apical segments in a medium containing the lowest combination of BAP and NAA or free of these growth regulators. The higher number of roots occurred in aerial shoots obtained from cotyledon segments, with or without IBA. After acclimatization, 83% of the plants survived. Keywords: in vitro seed germination; micropropagation; growth regulators; light regimes. RESUMOCom o objetivo obter a propagação in vitro de Casearia sylvestris, árvore nativa das florestas brasileiras, estudos de germinação in vitro das sementes foram realizados e as plântulas assépticas obtidas foram usadas como doadoras de explantes na micropropagação. Sementes maduras e imaturas, armazenadas por 10 dias a 10 ou 25°C foram inoculadas em meio contendo 10% de sais de MS e mantidas na luz (fotoperíodo de 16 horas) ou escuro contínuo, a 25°C. Para sementes imaturas, testou-se ou não ácido giberélico (GA 3 ) na concentração de 1,5 mg l -1 . Das plântulas obtidas, segmentos cotiledonares e apicais foram retirados e cultivados em meio MS completo com cinco combinações de 6-benzilaminopurina (BAP) e ácido naftalenoacético (ANA): 0,0; 0,25; 0,5; 1,0; 2,0 e 0,0; 0025; 0,05; 0,1; 0,2 mg L -1 , respectivamente. As brotações aéreas formadas in vitro foram cultivadas em meio MS contendo 0,0 ou 1,5 mg L -1 de ácido Paranhos, J. T. et al. 1192indol-3-butírico (IBA) para o enraizamento. O maior percentual de germinação (60%) ocorreu em sementes maduras expostas à luz, independentemente dos demais tratamentos, podendo ser consideradas fotoblásticas positivas. A maior percentagem de germinação das sementes imaturas (27%) ocorreu quando previamente armazenadas a 10°C, com ou sem GA 3 . A indução de brotos aéreos foi mais eficiente em segmentos apicais e em meio contendo a menor combinação de BAP e ANA ou ...

show abstract

“…Aos 120 dias, foram encontrados efeitos significativos (p<0,05) da interação entre os fatores (aditivos x ANA) e dos efeitos principais de aditivos e de concentração de ANA, para o percentual de explantes com clorose foliar. Gomes et al (2010), o carvão ativado combinado com o AIB promove a formação de raízes em explantes de Maclura tinctoria Lumber, por reduzir a ação oxidante.…”

Section: Resultsunclassified

Micropropagação de sucupira-preta por meio de gemas axilares

Moura

Titon

Fernandes

et al. 2012

Pesq. agropec. bras.

View full text Add to dashboard Cite

Resumo -O objetivo deste trabalho foi avaliar o uso de reguladores de crescimento, aditivos antioxidantes, tipo de explantes e intensidade de cobertura de mudas aclimatizadas, na micropropagação de sucupira-preta (Bowdichia virgilioides) por meio de gemas axilares. Os explantes foram cultivados para a multiplicação em meio de cultura básico WPM, suplementado com concentrações de benzilaminopurina (BAP). No alongamento, os tratamentos foram combinações de ácido naftalenoacético (ANA) e BAP adicionadas ao meio. Para o enraizamento, as brotações foram colocadas em meio com concentrações de ácido indolbutírico, ou em meio com combinações dos aditivos polivinilpirrolidona e carvão ativado, em diferentes concentrações de ANA. As plantas foram transplantadas para copos de plástico, com substrato, e cobertas com saco de polietileno. Posteriormente, esses sacos foram retirados, ou perfurados, ou não retirados, o que constituiu os tratamentos de pré-aclimatização in vitro. A aclimatização ex vitro foi realizada após o período de pré-aclimatização in vitro. A sucupira-preta apresentou melhor micropropagação com emprego de: segmentos cotiledonares e 0,3 mg L -1 de BAP, na multiplicação; 0,3 mg L -1 de ANA e 0,03 mg L -1 de BAP, no alongamento; e carvão ativado (2,0 mg L -1 ), no enraizamento e na pré-aclimatização in vitro, com uso de cobertura de plástico transparente em torno da muda.Termos para indexação: Bowdichia virgilioides, cultura de tecidos, espécie nativa, propagação, reguladores de crescimento. Sucupira-preta micropropagation through axillary budsAbstract -The objective of this work was to evaluate the use of growth regulators, antioxidant additives, type of explants, and coverage intensity of acclimatized seedlings in the micropropagation of sucupira-preta (Bowdichia virgilioides) through axillary buds. Explants were cultured for multiplication on WPM medium, supplemented with concentrations of benzylaminopurine (BAP). In the elongation phase, treatments consisted of naphthaleneacetic acid (NAA) and BAP combinations added to the medium. For rooting, shoots were inoculated in medium containing concentrations of indolbutyric acid or combinations of the additives polyvinylpyrrolidone and charcoal, in different NAA concentrations. Plants were transplanted to plastic cups with substrate, and covered with polyethylene bags. Later, the bags were withdrawn, or perforated, or not withdrawn, which constituted the in vitro pre-acclimatization treatments. Ex vitro acclimatization was carried out after the period of in vitro pre-acclimatization. Sucupira-preta had the best micropropagation using: cotyledon segments and 0.3 mg L -1 BAP, for multiplication; 0.3 mg L -1 NAA and 0.03 mg L -1 BAP, for elongation; and 2,0 mg L -1 activated carbon for rooting and for the in vitro pre-acclimatization, with the use of a transparent plastic cover around seedling.Index terms: Bowdichia virgilioides, tissue culture, native species, propagation, growth regulators. IntroduçãoA sucupira-preta (Bowdichia virgilioides Kunth.) é uma espécie ...

show abstract

Micropropagation of Maclura tinctoria L.: an endangered woody species

Cited by 18 publications

References 18 publications

In vitro establishment and early development of barueiro (Dipteryx alata Vogel)

In vitro establishment and early development of barueiro (Dipteryx alata Vogel)

PROPAGAÇÃO IN VITRO DE <i>Casearia sylvestris</i> SWARTZ (SALICACEAE)

Micropropagação de sucupira-preta por meio de gemas axilares

Contact Info

Product

Resources

About