Micropropagation of Malus Sieboldii Hybrids Resistant to Apple Proliferation Disease

Ciccotti, A.M.; Bisognin, C.; Battocletti, I.; Salvadori, A.; Herdemertens, M.; Wallbraun, M.; Jarausch, W.

doi:10.17660/actahortic.2009.839.1

Cited by 6 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nearly 75% rooting was achieved on 12 MS medium with 0.1-0.5 mg/L IBA, 2% sucrose and 0.4% agar in one step. Inducing Malus seiboldii genotypes at night with 25 M IBA in liquid or agarized medium increased rooting % (51). Various concentrations of IBA initiate roots, but 2.0 and 2.5 mg/L in MM106 show the most (68).…”

Section: In Vitro Rootingmentioning

confidence: 95%

“…MS, QL, WPM and DKW used for multiplication of Malus seiboldii and MS medium with an addition of iron showed highest proliferation rates ranged from 3.3 to 5.7 as well as shoot number (4.8 shoots/ explant) and shoot height i.e. 2.4 cm (51). In a study, (52) found that the MS medium supplemented with 2.22 M BAP produced the most shoots (6.8 + 0.80), the longest shoots (17.5 + 4.6 mm) and the highest sprouting % (85.0 %).…”

Section: In Vitro Shoot Proliferationmentioning

confidence: 99%

“…Douce de Djerba micropropagation earlier studied (26), revealed that regenerated shoots of apple had a 60% survival rate when transported to the field. For the Malus Seiboldii genotypes studied by (51), an acclimatization regimen implemented in the greenhouse yields survival rates of between 90 and 100 % for ex vitro plants grown in the greenhouse. The survival rate of transplanted in vitro produced M9 plants to polyvinyl cups or polybags was re-ported to be 80 % (52) under a carefully controlled decreasing RH regime of 95 % to 70+5 % over a period of 5+1 weeks.…”

Section: Acclimatization / Hardeningmentioning

confidence: 99%

See 2 more Smart Citations

Micropropagation of fruit crops: a review

et al. 2022

View full text Add to dashboard Cite

In vitro culture has the greatest commercial value in the extremely quick creation of clonal plants compared to conventional techniques of propagation. It has also proven to be of tremendous practical utility as assistance to the development and maintenance of disease-free planting material, germplasm conservation, and supplements to the regular methods of plant progress, such as plant genetic engineering. The discovery and implementation of current tissue culture techniques are thought to be paving the way for a second green revolution. Here, the standardization of techniques for the in vitro clonal propagation of fruit crops is highlighted. In addition, we summarize the effects of several surface sterilizing agents, plant growth regulators (PGRs), and anti-phenolic chemicals on culture establishment, shoot proliferation, in vitro rooting, hardening and economics of in vitro generation of true-to-type plants.

show abstract

Section: In Vitro Rootingmentioning

confidence: 95%

Section: In Vitro Shoot Proliferationmentioning

confidence: 99%

Section: Acclimatization / Hardeningmentioning

confidence: 99%

See 1 more Smart Citation

Micropropagation of fruit crops: a review

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Furthermore, this technique is labour-intensive and timeconsuming (Krahulcova´and Suda 2006). As in vitro cultures of the breeding materials were established for other purposes such as micropropagation of promising progeny genotypes (Ciccotti et al 2009), flow cytometry was preferred in our work and the polyploid status of M. sieboldii and its descendents was assessed. SSR markers at most polymorphic loci resulted also reliable in predicting the three different ploidy levels of the progeny.…”

Section: Discussionmentioning

confidence: 99%

Use of SSR markers to assess sexual vs. apomictic origin and ploidy level of breeding progeny derived from crosses of apple proliferation‐resistant Malus sieboldii and its hybrids with Malus × domestica cultivars

et al. 2009

Self Cite

View full text Add to dashboard Cite

To obtain apple rootstocks resistant to apple proliferation and suitable to modern fruit growing, 24 cross-combinations were performed over a 5-year period using Malus sieboldii and its hybrids as donors of the resistance trait and standard apple rootstock Malus · domestica genotypes as donors of agronomic value. Breeding with these genotypes was achieved despite different degrees of apomixis and polyploidy. Sets of five to six locus-specific microsatellite markers were identified for characterizing each progeny. Supported by flow cytometry these markers were applied to infer mode of reproduction, genomic constitution and ploidy level. Microsatellites allele composition identical to the maternal parent was revealed in 1668 of 3032 seedlings indicating seed formation through apomixis. Complete genetic recombination was found in 398 seedlings. The remaining hybrids displayed a higher ploidy than that of the parental plants which was consistent with the fertilization of unreduced egg cells. Thus, for each cross-combination, microsatellite loci were identified which enabled a reliable prediction of the ploidy level. They can now be applied in routine screening to distinguish sexual from apomictic progeny.

show abstract

“…In vitro cultures of healthy (control) and infected ‘Golden Delicious’ were established and propagated according to the protocol of Jarausch et al (1996) and Ciccotti et al (2008). Cultures were incubated in a growth chamber at 23 ± 2 °C under 16 h photoperiod with cool-white fluorescent light (60 μE m −2 s −1 ).…”

Section: Methodsmentioning

confidence: 99%

A gene expression analysis of cell wall biosynthetic genes in Malus x domestica infected by 'Candidatus Phytoplasma mali'

et al. 2012

View full text Add to dashboard Cite

Apple proliferation (AP) represents a serious threat to several fruit-growing areas and is responsible for great economic losses. Several studies have highlighted the key role played by the cell wall in response to pathogen attack. The existence of a cell wall integrity signaling pathway which senses perturbations in the cell wall architecture upon abiotic/biotic stresses and activates specific defence responses has been widely demonstrated in plants. More recently a role played by cell wall-related genes has also been reported in plants infected by phytoplasmas. With the aim of shedding light on the cell wall response to AP disease in the economically relevant fruit-tree Malus × domestica Borkh., we investigated the expression of the cellulose (CesA) and callose synthase (CalS) genes in different organs (i.e., leaves, roots and branch phloem) of healthy and infected symptomatic outdoor-grown trees, sampled over the course of two time points (i.e., spring and autumn 2011), as well as in in vitro micropropagated control and infected plantlets. A strong up-regulation in the expression of cell wall biosynthetic genes was recorded in roots from infected trees. Secondary cell wall CesAs showed up-regulation in the phloem tissue from branches of infected plants, while either a down-regulation of some genes or no major changes were observed in the leaves. Micropropagated plantlets also showed an increase in cell wall-related genes and constitute a useful system for a general assessment of gene expression analysis upon phytoplasma infection. Finally, we also report the presence of several 'knot'-like structures along the roots of infected apple trees and discuss the occurrence of this interesting phenotype in relation to the gene expression results and the modalities of phytoplasma diffusion.

show abstract

Micropropagation of Malus Sieboldii Hybrids Resistant to Apple Proliferation Disease

Cited by 6 publications

References 0 publications

Micropropagation of fruit crops: a review

Micropropagation of fruit crops: a review

Use of SSR markers to assess sexual vs. apomictic origin and ploidy level of breeding progeny derived from crosses of apple proliferation‐resistant Malus sieboldii and its hybrids with Malus × domestica cultivars

A gene expression analysis of cell wall biosynthetic genes in Malus x domestica infected by 'Candidatus Phytoplasma mali'

Contact Info

Product

Resources

About