Micropropagation of Medicinal Plants: a Review

Chatterjee, Tuhin; Ghosh, Biswajit

doi:10.23910/2/2020.0368

Cited by 9 publications

(2 citation statements)

References 19 publications

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“…The scientific community agrees and recommends the use of several different techniques to assess the phenotypic, cytological, phytochemical, and genetic profiles of the in vitro regenerated plants. [35].…”

Section: Somaclonal Variationmentioning

confidence: 99%

Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants

Duta-Cornescu

Constantin

Pojoga

et al. 2023

IJMS

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Cell and tissue plant cultures are used either to save vulnerable species from extinction or to multiply valuable genotypes, or both, and are widely applied for economically important plant species. For medicinal plants, the use of in vitro technologies for the production of secondary metabolites and pathogen-free plants has been greatly developed. Two opposite aspects characterize the in vitro micropropagation of medicinal plants: maintaining genetic fidelity for the perpetuation and preservation of elites, and the identification and exploitation of somaclonal variations associated with new, useful traits. A balance between what is advantageous and what is undesirable is necessary, and this implies the identification of somaclonal variability at all levels, from the phenotypic to molecular ones. This review addresses the somaclonal variation arising from the in vitro multiplication of medicinal plants from three perspectives: cytogenetics, genetics, and epigenetics. The possible causes of the appearance of somaclones, the methods for their identification, and the extent to which they are desirable are presented comparatively for different plant species with therapeutic properties. The emphasis is on the subtle changes at the genetic and epigenetic level, as it results from the application of methods based on DNA markers.

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Section: Somaclonal Variationmentioning

confidence: 99%

Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants

Duta-Cornescu

Constantin

Pojoga

et al. 2023

IJMS

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“…Gloriosa superb is a good abortifacient 4 . Its seeds and tubers are used mainly for treating gout and rheumatism 5 . Roots are purgative, cholagogue, antihelminthic, astringent and germicidal.…”

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confidence: 99%

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2023

IJPSR

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Background: Traditionally Gloriosa superba is being used as a medicinal plant in outskirts of belagavi, we evaluated and compared the analgesic and anti-inflammatory activities of hydroalcoholic extract of dried aerial parts (rhizomes) of G. superba with standard drugs. Methods: This experimental animal study was carried out in the Research Laboratory, Department of Pharmacology, BIMS, Belagavi. Prior permission from institutional ethical committee and institutional animal ethics committee was taken. Albino rats and Albino mice were used. Acute toxicity study was also done according to OECD guideline no. 425 and test doses were decided accordingly. The experimental models of Eddy's hot plate method was used to study the analgesic activity whereas formalin induced peritonitis and cotton pellet granuloma models were used for anti-inflammatory action. Statistical analysis was performed using Mann-whitney U test and chi-square test (SPSS software version 22). Results: In eddy's hot plate method, for G. superba extract of 400mg/kg, percentage increase in reaction time of at 120 mins was 60.49%, in formalin induces peritonitis method, for G. superba extract of 400mg/kg, percentage inhibition of peritoneal exudate was 47.14%. In cotton pellet induced granuloma method, for G. superba extract of 400mg/kg, percentage inhibition of granuloma formation was 50.32%. All the results of 4 different groups in each method showed significant difference in their respective pharmacological activities (Chi Square values: 14.4, 14.4, 75.2). Conclusion: Hydroalcoholic extract of G. superba has shown significant analgesic and anti-inflammatory activity in our study. INTRODUCTION:Pain is an unpleasant sensation and emotional experience associated with actual or potential tissue damage or described in terms of such damage 1 . The pathophysiology of pain involves 2 components, peripheral nociception and central mechanism.

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